Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_1 [P05119]  : Wound-induced proteinase inhibitor 2 (Wound-induced proteinase inhibitor II)

148Length

Solanum lycopersicumSpecie

-Gene Name

16293Mass

3Domain Count

Sequence :
     FASTA
MAVHKEVNFV AYLLIVLGMF LYVDAKACTR ECGNLGFGIC PRSEGSPLNP ICINCCSGYK GCNYYNSFGK 70
FICEGESDPK RPNACTFNCD PNIAYSRCPR SQGKSLIYPT GCTTCCTGYK GCYYFGKDGK FVCEGESDEP 140
KANMYPVM 148
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKEVNFVAYLLIVLGMFLYVDA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-62
50 - 101 52
IRD-405
83 - 135 53
IRD-456
26 - 75 50
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.879
3.2 -4.8 -10.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
P05119 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 K03291
REFERENCES
PubMed ID:     3838986    | 12684499    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-4 CTREC Trypsin 29 - 33
RL-7 CTFNC Chymotrypsin 86 - 90
RL-7 CTFNC Chymotrypsin 86 - 90
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) extracellular region (GO:0005576) None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves Wounding Bi-domain TI-II 3838986
Domain I consists of the first 15 residues and residues 86 to 116, whereas Domain II consists of residues 16–73, clasped bracelet fold 12684499
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
chymotrypsin (0.03), trypsin (0.08) Subtilisin, chymotrypsin, trypsin Chymotrypsin, trypsin 12684499
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
1 4 12684499
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-62 50 - 101 CTFNC 85 - 89 DPKRP 78 - 82 H-L Type (Type-I)
IRD-405 83 - 135 CTFNC 85 - 89 - H+L Type (Type-III)
IRD-456 26 - 75 CTREC 28 - 32 EGSPL 44 - 48 L-H Type (Type-II)
PICINCCSGYKGCNYYNSFGKFICEGESDPKRPNACTFNCDPNIAYSRCPRS
CTFNC
DPKRP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C I N C C S G Y K G C N Y Y N S F G K F I C E G E S D P K R P N A C T F N C D P N I A Y S R C P R S


NACTFNCDPNIAYSRCPRSQGKSLIYPTGCTTCCTGYKGCYYFGKDGKFVCEG
CTFNC
H+L Type (Type-III)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N A C T F N C D P N I A Y S R C P R S Q G K S L I Y P T G C T T C C T G Y K G C Y Y F G K D G K F V C E G


KACTRECGNLGFGICPRSEGSPLNPICINCCSGYKGCNYYNSFGKFICEG
CTREC
EGSPL
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T R E C G N L G F G I C P R S E G S P L N P I C I N C C S G Y K G C N Y Y N S F G K F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-62
 50 - 101 52
IRD-405
 83 - 135 53
IRD-456
 26 - 75 50
Amino Acid Count % by Frequency
Alanine 7 4.73
Cysteine 16 10.81
Aspartic acid 5 3.38
Glutamic acid 8 5.41
Phenylalanine 8 5.41
Glycine 16 10.81
Histidine 1 0.68
Isoleucine 7 4.73
Lysine 10 6.76
Leucine 7 4.73
Methionine 4 2.7
Asparagine 10 6.76
Proline 10 6.76
Glutamine 1 0.68
Arginine 5 3.38
Serine 9 6.08
Threonine 6 4.05
Valine 7 4.73
Tryptophan 0 0
Tyrosine 11 7.43