Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_118 [T1PZF9]  : Pin-II type proteinase inhibitor 65

204Length

Capsicum annuumSpecie

-Gene Name

22302Mass

5Domain Count

Sequence :
     FASTA
MAVPKEVSFL ASLLVLGILL LHVDAKACSQ RNAKEPICTN CCAGRKGCNY YSADGTFICE GESDPNNPKP 70
CTLNCDPRIF YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNHPKACP RNCDPRIFYS 140
KCPRSEGNAE NRICTNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDPNIAYSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLASLLVLGILLLHVDA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-93
152 - 203 52
IRD-115
94 - 145 52
IRD-121
36 - 87 52
IRD-546
127 - 177 51
IRD-551
69 - 119 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.558
3.1 -9.5 -18.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
T1PZF9 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 JX106515
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
RL-3 CTLNC Chymotrypsin 72 - 76
RL-3 CTLNC Chymotrypsin 72 - 76
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-93 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-115 94 - 145 CPRNC 129 - 133 DPNHP 122 - 126 H-L Type (Type-I)
IRD-121 36 - 87 CTLNC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-546 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
IRD-551 69 - 119 CTLNC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N I A Y S L C L Y E


RICTNCCAGRKGCNYYSADGTFICEGESDPNHPKACPRNCDPRIFYSKCPRS
CPRNC
DPNHP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N H P K A C P R N C D P R I F Y S K C P R S


PICTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCTLNCDPRIFYSKCPRS
CTLNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C T L N C D P R I F Y S K C P R S


KACPRNCDPRIFYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I F Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


KPCTLNCDPRIFYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CTLNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T L N C D P R I F Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-93
 152 - 203 52
IRD-115
 94 - 145 52
IRD-121
 36 - 87 52
IRD-546
 127 - 177 51
IRD-551
 69 - 119 51
Amino Acid Count % by Frequency
Alanine 16 7.84
Cysteine 25 12.25
Aspartic acid 10 4.9
Glutamic acid 13 6.37
Phenylalanine 6 2.94
Glycine 15 7.35
Histidine 2 0.98
Isoleucine 10 4.9
Lysine 12 5.88
Leucine 10 4.9
Methionine 1 0.49
Asparagine 20 9.8
Proline 16 7.84
Glutamine 1 0.49
Arginine 12 5.88
Serine 14 6.86
Threonine 7 3.43
Valine 4 1.96
Tryptophan 0 0
Tyrosine 10 4.9