Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_12 [Q00782]  : Proteinase inhibitor type-2 (Proteinase inhibitor type II)

158Length

Solanum tuberosumSpecie

-Gene Name

17132Mass

4Domain Count

Sequence :
     FASTA
MAIHKEVSFL AYLLVLGMLL FVSAMEHVDA KACTLECGNL GYGICPRSEG SPENPICTNC CAGYKGCNYY 70
SANGTFICEG QSHPKNPKAC PRNCDPHIAY SKCPRSGGKT LIYPTGCTTC CTGYTDCYYF GKDGKFVCEG 140
ESIEPKACTL ECDSRLHT 158
SIGNAL PEPTIDE
SEQUENCE Position
MAIHKEVSFLAYLLVLGMLLFVSA
 1 ↔ 24
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-9
115 - 157 43
IRD-46
55 - 101 47
IRD-464
31 - 80 50
IRD-484
88 - 140 53
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.16299999999999
2.6 -8.6 -15.1
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q00782 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 Z12753
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-9 CTLEC Chymotrypsin 34 - 38
RL-9 CTLEC Chymotrypsin 149 - 153
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-9 115 - 157 CTLEC 148 - 152 - H+L Type (Type-III)
IRD-46 55 - 101 CPRNC 90 - 94 HPKNP 83 - 87 H-L Type (Type-I)
IRD-464 31 - 80 CTLEC 33 - 37 EGSPE 49 - 53 L-H Type (Type-II)
IRD-484 88 - 140 CPRNC 90 - 94 - H+L Type (Type-III)
TGCTTCCTGYTDCYYFGKDGKFVCEGESIEPKACTLECDSRLH
CTLEC
H+L Type (Type-III)
3,24 ; 7,38 ; 13,34
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43
T G C T T C C T G Y T D C Y Y F G K D G K F V C E G E S I E P K A C T L E C D S R L H


PICTNCCAGYKGCNYYSANGTFICEGQSHPKNPKACPRNCDPHIAYS
CPRNC
HPKNP
H-L Type (Type-I)
7,36 ; 24,40
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47
P I C T N C C A G Y K G C N Y Y S A N G T F I C E G Q S H P K N P K A C P R N C D P H I A Y S


KACTLECGNLGYGICPRSEGSPENPICTNCCAGYKGCNYYSANGTFICEG
CTLEC
EGSPE
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T L E C G N L G Y G I C P R S E G S P E N P I C T N C C A G Y K G C N Y Y S A N G T F I C E G


KACPRNCDPHIAYSKCPRSGGKTLIYPTGCTTCCTGYTDCYYFGKDGKFVCEG
CPRNC
H+L Type (Type-III)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
K A C P R N C D P H I A Y S K C P R S G G K T L I Y P T G C T T C C T G Y T D C Y Y F G K D G K F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-9
 115 - 157 43
IRD-46
 55 - 101 47
IRD-464
 31 - 80 50
IRD-484
 88 - 140 53
Amino Acid Count % by Frequency
Alanine 10 6.33
Cysteine 18 11.39
Aspartic acid 5 3.16
Glutamic acid 10 6.33
Phenylalanine 5 3.16
Glycine 16 10.13
Histidine 5 3.16
Isoleucine 7 4.43
Lysine 10 6.33
Leucine 11 6.96
Methionine 3 1.9
Asparagine 7 4.43
Proline 10 6.33
Glutamine 1 0.63
Arginine 4 2.53
Serine 10 6.33
Threonine 11 6.96
Valine 5 3.16
Tryptophan 0 0
Tyrosine 10 6.33