Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_120 [T1PZB4]  : Pin-II type proteinase inhibitor 49

262Length

Capsicum annuumSpecie

-Gene Name

28563Mass

7Domain Count

Sequence :
     FASTA
MAVPKEVSFL ASLLVLGILL LHVDAKACSQ RNAKEPICTN CCAGRKGCNY YSADGTFICE GESDPNNPKP 70
CTLNCDPRIF YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKACP RYCDTRIAYS 140
KCPRSEGNAE NRICTNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDTRIAYSKC PRSEGNAENR 210
LCTNCCAGRK GCNYYSADGT FICEGKSDPN NPKACPRNCD PNIAYSLCLY EK 262
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLASLLVLGILLLHVDA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-83
152 - 203 52
IRD-89
210 - 261 52
IRD-121
36 - 87 52
IRD-133
94 - 145 52
IRD-536
185 - 235 51
IRD-551
69 - 119 51
IRD-559
127 - 177 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.934
5.8 -9.9 -21.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
T1PZB4 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 JX106499
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 188 - 192
RL-1 CPRNC Trypsin 188 - 192
RL-1 CPRNC Trypsin 246 - 250
RL-3 CTLNC Chymotrypsin 72 - 76
RL-3 CTLNC Chymotrypsin 72 - 76
RL-6 CPRYC Trypsin 130 - 134
RL-6 CPRYC Trypsin 130 - 134
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-83 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-89 210 - 261 CPRNC 245 - 249 DPNNP 238 - 242 H-L Type (Type-I)
IRD-121 36 - 87 CTLNC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-133 94 - 145 CPRYC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-536 185 - 235 CPRNC 187 - 191 EGNAE 204 - 208 L-H Type (Type-II)
IRD-551 69 - 119 CTLNC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
IRD-559 127 - 177 CPRYC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D T R I A Y S K C P R S


RLCTNCCAGRKGCNYYSADGTFICEGKSDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y Y S A D G T F I C E G K S D P N N P K A C P R N C D P N I A Y S L C L Y E


PICTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCTLNCDPRIFYSKCPRS
CTLNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C T L N C D P R I F Y S K C P R S


RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R Y C D T R I A Y S K C P R S


KACPRNCDTRIAYSKCPRSEGNAENRLCTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y S K C P R S E G N A E N R L C T N C C A G R K G C N Y Y S A D G T F I C E G


KPCTLNCDPRIFYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CTLNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T L N C D P R I F Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


KACPRYCDTRIAYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRYC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R Y C D T R I A Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-83
 152 - 203 52
IRD-89
 210 - 261 52
IRD-121
 36 - 87 52
IRD-133
 94 - 145 52
IRD-536
 185 - 235 51
IRD-551
 69 - 119 51
IRD-559
 127 - 177 51
Amino Acid Count % by Frequency
Alanine 22 8.4
Cysteine 33 12.6
Aspartic acid 13 4.96
Glutamic acid 16 6.11
Phenylalanine 6 2.29
Glycine 20 7.63
Histidine 1 0.38
Isoleucine 12 4.58
Lysine 16 6.11
Leucine 11 4.2
Methionine 1 0.38
Asparagine 27 10.31
Proline 19 7.25
Glutamine 1 0.38
Arginine 17 6.49
Serine 18 6.87
Threonine 11 4.2
Valine 4 1.53
Tryptophan 0 0
Tyrosine 14 5.34