Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_126 [Q9SDW7]  : Proteinase inhibitor type II NGPI-2 (Fragment)

390Length

Nicotiana glutinosaSpecie

-Gene Name

42785Mass

11Domain Count

Sequence :
     FASTA
VHRASFRALL LLFGISLLVS TVEHADAKAC PRNCDRRIAY GICPRSEEKN NDRICTNCCA GMKGCNYFSA 70
DGTFVCEGES GPKNPKACPR NCDGRIAYGV CPRKEEKKNN WICTNCCAGT KGCNYFSADG TFVCEGESDP 140
RNPKACPRNC DPRISDEICP RSEEKNNNQI CTNCCAGTKG CNYFSADGTF VCEGEYDPKN PKACPRNCDP 210
RISYGVCPRS EEKNNNQICT NCCAGTKGCN YFSADGTFVC EGEYDPKNPK ACPRNCDGRI DYGVCPRSEE 280
KKNNQICTNC CAGTKGCNYF SADGTFVCEG ESDPRNPKAC PLNCDGRIAY EICPRTEEKK TNQICTNCCA 350
GTKGCNYFNA NGTFICEGES EYVNKVENDL EKSKVLFSKS 390
SIGNAL PEPTIDE
SEQUENCE Position
VHRASFRALLLLFGISLLVSTVEHADA
 1 ↔ 27
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-160
53 - 104 52
IRD-162
111 - 162 52
IRD-163
169 - 220 52
IRD-164
227 - 278 52
IRD-241
285 - 336 52
IRD-440
318 - 368 51
IRD-579
28 - 78 51
IRD-580
144 - 194 51
IRD-581
202 - 252 51
IRD-611
86 - 136 51
IRD-612
260 - 310 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.56
5.5 -18.3 -35.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q9SDW7 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF205852
REFERENCES
PubMed ID:     11004491    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 31 - 35
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 263 - 267
RL-1 CPRNC Trypsin 263 - 267
RL-2 CPLNC Chymotrypsin 321 - 325
RL-2 CPLNC Chymotrypsin 321 - 325
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-160 53 - 104 CPRNC 88 - 92 GPKNP 81 - 85 H-L Type (Type-I)
IRD-162 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-163 169 - 220 CPRNC 204 - 208 DPKNP 197 - 201 H-L Type (Type-I)
IRD-164 227 - 278 CPRNC 262 - 266 DPKNP 255 - 259 H-L Type (Type-I)
IRD-241 285 - 336 CPLNC 320 - 324 DPRNP 313 - 317 H-L Type (Type-I)
IRD-440 318 - 368 CPLNC 320 - 324 EEKKT 337 - 341 L-H Type (Type-II)
IRD-579 28 - 78 CPRNC 30 - 34 EEKNN 47 - 51 L-H Type (Type-II)
IRD-580 144 - 194 CPRNC 146 - 150 EEKNN 163 - 167 L-H Type (Type-II)
IRD-581 202 - 252 CPRNC 204 - 208 EEKNN 221 - 225 L-H Type (Type-II)
IRD-611 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-612 260 - 310 CPRNC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
RICTNCCAGMKGCNYFSADGTFVCEGESGPKNPKACPRNCDGRIAYGVCPRK
CPRNC
GPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E S G P K N P K A C P R N C D G R I A Y G V C P R K


WICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDPRISDEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
W I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D P R I S D E I C P R S


QICTNCCAGTKGCNYFSADGTFVCEGEYDPKNPKACPRNCDPRISYGVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E Y D P K N P K A C P R N C D P R I S Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGEYDPKNPKACPRNCDGRIDYGVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E Y D P K N P K A C P R N C D G R I D Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPLNCDGRIAYEICPRT
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P L N C D G R I A Y E I C P R T


KACPLNCDGRIAYEICPRTEEKKTNQICTNCCAGTKGCNYFNANGTFICEG
CPLNC
EEKKT
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D G R I A Y E I C P R T E E K K T N Q I C T N C C A G T K G C N Y F N A N G T F I C E G


KACPRNCDRRIAYGICPRSEEKNNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D R R I A Y G I C P R S E E K N N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDPRISDEICPRSEEKNNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I S D E I C P R S E E K N N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDPRISYGVCPRSEEKNNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I S Y G V C P R S E E K N N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRKEEKKNNWICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R K E E K K N N W I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIDYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I D Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-160
 53 - 104 52
IRD-162
 111 - 162 52
IRD-163
 169 - 220 52
IRD-164
 227 - 278 52
IRD-241
 285 - 336 52
IRD-440
 318 - 368 51
IRD-579
 28 - 78 51
IRD-580
 144 - 194 51
IRD-581
 202 - 252 51
IRD-611
 86 - 136 51
IRD-612
 260 - 310 51
Amino Acid Count % by Frequency
Alanine 25 6.41
Cysteine 48 12.31
Aspartic acid 20 5.13
Glutamic acid 30 7.69
Phenylalanine 15 3.85
Glycine 33 8.46
Histidine 2 0.51
Isoleucine 17 4.36
Lysine 29 7.44
Leucine 9 2.31
Methionine 1 0.26
Asparagine 40 10.26
Proline 24 6.15
Glutamine 4 1.03
Arginine 23 5.9
Serine 21 5.38
Threonine 20 5.13
Valine 14 3.59
Tryptophan 1 0.26
Tyrosine 14 3.59