Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_128 [Q9SDW8]  : Proteinase inhibitor type II NGPI-1

506Length

Nicotiana glutinosaSpecie

-Gene Name

55352Mass

15Domain Count

Sequence :
     FASTA
MAVHRASFRA LLLLFGISLL VSTVEHADAK ACPRNCDRRI AYGICPRSEE KNNDRICTNC CAGMKGCNYF 70
SADGTFVCEG ESGPKNPKAC PRNCDGRIAY GVCPRKEEKK NNWICTNCCA GMKGCNYFSA DGTFVCEGES 140
GPKNPKACPR NCDGRIAYGV CPRKEEKKNN WICTNCCAGM KGCNYFSADG TFVCEGESGP KNPKACPRNC 210
DGRIAYGVCP RKEEKKNNWI CTNCCAGTKG CNYFSADGTF VCEGESDPRN PKACPRNCDP RISDEICPRS 280
EEKNNNQICT NCCAGTKGCN YFSADGTFVC EGEYDPKNPK ACPRNCDPRI SYGVCPRSEE KNNNQICTNC 350
CAGTKGCNYF SADGTFVCEG EYDPKNPKAC PRNCDGRIDY GVCPRSEEKK NNQICTNCCA GTKGCNYFSA 420
DGTFVCEGES DPRNPKACPL NCDGRIAYEI CPRTEEKKTN QICTNCCAGT KGCNYFNANG TFICEGESEY 490
VNKVENDLEK SKVAVF 506
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRASFRALLLLFGISLLVSTVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-160
55 - 106 52
IRD-161
113 - 164 52
IRD-161
171 - 222 52
IRD-162
229 - 280 52
IRD-163
287 - 338 52
IRD-164
345 - 396 52
IRD-241
403 - 454 52
IRD-440
436 - 486 51
IRD-579
30 - 80 51
IRD-580
262 - 312 51
IRD-581
320 - 370 51
IRD-610
146 - 196 51
IRD-610
88 - 138 51
IRD-611
204 - 254 51
IRD-612
378 - 428 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.91799999999999
11 -20.2 -43.4
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q9SDW8 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF205851
REFERENCES
PubMed ID:     11004491    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 381 - 385
RL-1 CPRNC Trypsin 381 - 385
RL-2 CPLNC Chymotrypsin 439 - 443
RL-2 CPLNC Chymotrypsin 439 - 443
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-160 55 - 106 CPRNC 90 - 94 GPKNP 83 - 87 H-L Type (Type-I)
IRD-161 113 - 164 CPRNC 148 - 152 GPKNP 141 - 145 H-L Type (Type-I)
IRD-161 171 - 222 CPRNC 206 - 210 GPKNP 199 - 203 H-L Type (Type-I)
IRD-162 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-163 287 - 338 CPRNC 322 - 326 DPKNP 315 - 319 H-L Type (Type-I)
IRD-164 345 - 396 CPRNC 380 - 384 DPKNP 373 - 377 H-L Type (Type-I)
IRD-241 403 - 454 CPLNC 438 - 442 DPRNP 431 - 435 H-L Type (Type-I)
IRD-440 436 - 486 CPLNC 438 - 442 EEKKT 455 - 459 L-H Type (Type-II)
IRD-579 30 - 80 CPRNC 32 - 36 EEKNN 49 - 53 L-H Type (Type-II)
IRD-580 262 - 312 CPRNC 264 - 268 EEKNN 281 - 285 L-H Type (Type-II)
IRD-581 320 - 370 CPRNC 322 - 326 EEKNN 339 - 343 L-H Type (Type-II)
IRD-610 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-610 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-611 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-612 378 - 428 CPRNC 380 - 384 EEKKN 397 - 401 L-H Type (Type-II)
RICTNCCAGMKGCNYFSADGTFVCEGESGPKNPKACPRNCDGRIAYGVCPRK
CPRNC
GPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E S G P K N P K A C P R N C D G R I A Y G V C P R K


WICTNCCAGMKGCNYFSADGTFVCEGESGPKNPKACPRNCDGRIAYGVCPRK
CPRNC
GPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
W I C T N C C A G M K G C N Y F S A D G T F V C E G E S G P K N P K A C P R N C D G R I A Y G V C P R K


WICTNCCAGMKGCNYFSADGTFVCEGESGPKNPKACPRNCDGRIAYGVCPRK
CPRNC
GPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
W I C T N C C A G M K G C N Y F S A D G T F V C E G E S G P K N P K A C P R N C D G R I A Y G V C P R K


WICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDPRISDEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
W I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D P R I S D E I C P R S


QICTNCCAGTKGCNYFSADGTFVCEGEYDPKNPKACPRNCDPRISYGVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E Y D P K N P K A C P R N C D P R I S Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGEYDPKNPKACPRNCDGRIDYGVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E Y D P K N P K A C P R N C D G R I D Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPLNCDGRIAYEICPRT
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P L N C D G R I A Y E I C P R T


KACPLNCDGRIAYEICPRTEEKKTNQICTNCCAGTKGCNYFNANGTFICEG
CPLNC
EEKKT
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D G R I A Y E I C P R T E E K K T N Q I C T N C C A G T K G C N Y F N A N G T F I C E G


KACPRNCDRRIAYGICPRSEEKNNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D R R I A Y G I C P R S E E K N N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDPRISDEICPRSEEKNNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I S D E I C P R S E E K N N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDPRISYGVCPRSEEKNNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I S Y G V C P R S E E K N N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRKEEKKNNWICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R K E E K K N N W I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRKEEKKNNWICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R K E E K K N N W I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRKEEKKNNWICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R K E E K K N N W I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIDYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I D Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-160
 55 - 106 52
IRD-161
 113 - 164 52
IRD-161
 171 - 222 52
IRD-162
 229 - 280 52
IRD-163
 287 - 338 52
IRD-164
 345 - 396 52
IRD-241
 403 - 454 52
IRD-440
 436 - 486 51
IRD-579
 30 - 80 51
IRD-580
 262 - 312 51
IRD-581
 320 - 370 51
IRD-610
 146 - 196 51
IRD-610
 88 - 138 51
IRD-611
 204 - 254 51
IRD-612
 378 - 428 51
Amino Acid Count % by Frequency
Alanine 35 6.92
Cysteine 64 12.65
Aspartic acid 24 4.74
Glutamic acid 38 7.51
Phenylalanine 19 3.75
Glycine 47 9.29
Histidine 2 0.4
Isoleucine 21 4.15
Lysine 40 7.91
Leucine 8 1.58
Methionine 4 0.79
Asparagine 52 10.28
Proline 32 6.32
Glutamine 4 0.79
Arginine 29 5.73
Serine 23 4.55
Threonine 24 4.74
Valine 19 3.75
Tryptophan 3 0.59
Tyrosine 18 3.56