Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_130 [Q1WL51]  : Trypsin proteinase inhibitor

163Length

Nicotiana cavicolaSpecie

PIGene Name

17880Mass

3Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLSFGMSLL VSNVEHAKAC TRECDTRIAY GVCPRLEEKK NNRLCTNCCS GTKGCNYFSA 70
DGTFVCEGES DPRNPKGCTF ECDPRIAYGI CPRSEEKKNN RLCTNCCAGK KGCNYFSSNG TFICEGESEY 140
ASKAVEYVGE VEDDLQKSKV AVS 163
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLSFGMS
 1 ↔ 18
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-262
53 - 104 52
IRD-664
28 - 78 51
IRD-671
86 - 136 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.395
2.3 -6.8 -12.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL51 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158181
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-4 CTREC Trypsin 31 - 35
RL-8 CTFEC Chymotrypsin 89 - 93
RL-8 CTFEC Chymotrypsin 89 - 93
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-262 53 - 104 CTFEC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-664 28 - 78 CTREC 30 - 34 EEKKN 47 - 51 L-H Type (Type-II)
IRD-671 86 - 136 CTFEC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
RLCTNCCSGTKGCNYFSADGTFVCEGESDPRNPKGCTFECDPRIAYGICPRS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C S G T K G C N Y F S A D G T F V C E G E S D P R N P K G C T F E C D P R I A Y G I C P R S


KACTRECDTRIAYGVCPRLEEKKNNRLCTNCCSGTKGCNYFSADGTFVCEG
CTREC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R E C D T R I A Y G V C P R L E E K K N N R L C T N C C S G T K G C N Y F S A D G T F V C E G


KGCTFECDPRIAYGICPRSEEKKNNRLCTNCCAGKKGCNYFSSNGTFICEG
CTFEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T F E C D P R I A Y G I C P R S E E K K N N R L C T N C C A G K K G C N Y F S S N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-262
 53 - 104 52
IRD-664
 28 - 78 51
IRD-671
 86 - 136 51
Amino Acid Count % by Frequency
Alanine 11 6.75
Cysteine 16 9.82
Aspartic acid 6 3.68
Glutamic acid 15 9.2
Phenylalanine 7 4.29
Glycine 13 7.98
Histidine 2 1.23
Isoleucine 4 2.45
Lysine 12 7.36
Leucine 10 6.13
Methionine 2 1.23
Asparagine 11 6.75
Proline 5 3.07
Glutamine 1 0.61
Arginine 9 5.52
Serine 14 8.59
Threonine 8 4.91
Valine 11 6.75
Tryptophan 0 0
Tyrosine 6 3.68