Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_137 [T1PZ91]  : Pin-II type proteinase inhibitor 43 (Pin-II type proteinase inhibitor 63)

204Length

Capsicum annuumSpecie

-Gene Name

22348Mass

5Domain Count

Sequence :
     FASTA
MAVPKEVSFL AFLLVLEILL LHVDAKACSE ENAENRICTN CCAGRKGCNY YSADGTFICE GESDPNNPKA 70
CPRYCDTRIA YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKACP RNCDTRIAYS 140
KCPRSEGNAE NRLCTNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDPNIAYSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFLLVLEILLLHVDA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-83
94 - 145 52
IRD-84
152 - 203 52
IRD-133
36 - 87 52
IRD-536
127 - 177 51
IRD-559
69 - 119 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.18
-0.2 -12.5 -21.4
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
T1PZ91 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 JX106493
JX106513
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
RL-6 CPRYC Trypsin 72 - 76
RL-6 CPRYC Trypsin 72 - 76
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-83 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-84 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-133 36 - 87 CPRYC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-536 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
IRD-559 69 - 119 CPRYC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D T R I A Y S K C P R S


RLCTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N I A Y S L C L Y E


RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R Y C D T R I A Y S K C P R S


KACPRNCDTRIAYSKCPRSEGNAENRLCTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y S K C P R S E G N A E N R L C T N C C A G R K G C N Y Y S A D G T F I C E G


KACPRYCDTRIAYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRYC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R Y C D T R I A Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-83
 94 - 145 52
IRD-84
 152 - 203 52
IRD-133
 36 - 87 52
IRD-536
 127 - 177 51
IRD-559
 69 - 119 51
Amino Acid Count % by Frequency
Alanine 19 9.31
Cysteine 25 12.25
Aspartic acid 10 4.9
Glutamic acid 16 7.84
Phenylalanine 5 2.45
Glycine 14 6.86
Histidine 1 0.49
Isoleucine 9 4.41
Lysine 11 5.39
Leucine 10 4.9
Methionine 1 0.49
Asparagine 21 10.29
Proline 13 6.37
Glutamine 0 0
Arginine 13 6.37
Serine 13 6.37
Threonine 8 3.92
Valine 4 1.96
Tryptophan 0 0
Tyrosine 11 5.39