Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_139 [A0A075EZT7]  : Proteinase inhibitor II (proteinase inhibitor type-2)

197Length

Nicotiana tabacumSpecie

cevi57 LOC107816122Gene Name

20985Mass

5Domain Count

Sequence :
     FASTA
MAVHKVSFVA HLLVLGMFLL LVDAKACTKE CGNFAYGICP RSQGTPDDPI CTTCCAGYKG CNYYSANGTF 70
ICEGSSDPKN PNVCPQFCDP DIAYSKCPRS EGETIINPTG CTTCCTGYKG CYYFGQDGEF VCEGESDEPK 140
SCTTECDPRV ATISCPFSGL VKINQECINC CNADKGCELY DNDGSLICTG GEPQSAA 197
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVSFVAHLLVLGMFLLLVDA
 1 ↔ 24
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-41
109 - 158 50
IRD-265
49 - 100 52
IRD-292
140 - 190 51
IRD-419
25 - 74 50
IRD-427
82 - 134 53
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.069
-8.9 -21.1 -29.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A075EZT7 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 KJ730269
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-10 CTKEC Trypsin 28 - 32
RL-12 CPQFC Unknown 85 - 89
RL-12 CPQFC Unknown 85 - 89
RL-24 CTTEC Unknown 143 - 147
RL-24 CTTEC Unknown 143 - 147
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-41 109 - 158 CTTEC 142 - 146 ESDEP 135 - 139 H-L Type (Type-I)
IRD-265 49 - 100 CPQFC 84 - 88 DPKNP 77 - 81 H-L Type (Type-I)
IRD-292 140 - 190 CTTEC 142 - 146 GLVKI 159 - 163 L-H Type (Type-II)
IRD-419 25 - 74 CTKEC 27 - 31 - H+L Type (Type-III)
IRD-427 82 - 134 CPQFC 84 - 88 EGETI 101 - 105 L-H Type (Type-II)
TGCTTCCTGYKGCYYFGQDGEFVCEGESDEPKSCTTECDPRVATISCPFS
CTTEC
ESDEP
H-L Type (Type-I)
3,38 ; 6,24 ; 7,34 ; 13,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G E S D E P K S C T T E C D P R V A T I S C P F S


PICTTCCAGYKGCNYYSANGTFICEGSSDPKNPNVCPQFCDPDIAYSKCPRS
CPQFC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T T C C A G Y K G C N Y Y S A N G T F I C E G S S D P K N P N V C P Q F C D P D I A Y S K C P R S


KSCTTECDPRVATISCPFSGLVKINQECINCCNADKGCELYDNDGSLICTG
CTTEC
GLVKI
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K S C T T E C D P R V A T I S C P F S G L V K I N Q E C I N C C N A D K G C E L Y D N D G S L I C T G


KACTKECGNFAYGICPRSQGTPDDPICTTCCAGYKGCNYYSANGTFICEG
CTKEC
H+L Type (Type-III)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T K E C G N F A Y G I C P R S Q G T P D D P I C T T C C A G Y K G C N Y Y S A N G T F I C E G


NVCPQFCDPDIAYSKCPRSEGETIINPTGCTTCCTGYKGCYYFGQDGEFVCEG
CPQFC
EGETI
L-H Type (Type-II)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N V C P Q F C D P D I A Y S K C P R S E G E T I I N P T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-41
 109 - 158 50
IRD-265
 49 - 100 52
IRD-292
 140 - 190 51
IRD-419
 25 - 74 50
IRD-427
 82 - 134 53
Amino Acid Count % by Frequency
Alanine 12 6.09
Cysteine 24 12.18
Aspartic acid 12 6.09
Glutamic acid 12 6.09
Phenylalanine 8 4.06
Glycine 20 10.15
Histidine 2 1.02
Isoleucine 10 5.08
Lysine 10 5.08
Leucine 9 4.57
Methionine 2 1.02
Asparagine 10 5.08
Proline 13 6.6
Glutamine 5 2.54
Arginine 3 1.52
Serine 13 6.6
Threonine 14 7.11
Valine 9 4.57
Tryptophan 0 0
Tyrosine 9 4.57