Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_143 [Q1WL41]  : Trypsin proteinase inhibitor

338Length

Nicotiana spegazziniiSpecie

PIGene Name

37486Mass

9Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSNVEYADAK ACPRNCDPRI AYGVCPRSEE KEDNQICTNC CAGRKGCNYF 70
SADGTFVCEG ESDPKNPKGC PRICDPRIAY RICRRSEEKK NDRICTNCCA GTKGCNYFSD DGTFVCEGES 140
DPRNPKACPR NCDPRIAYGI CRRSEEKKND RICTNCCAGT KGCKYFSYDG NFVCEGESDP TNPKACPRNC 210
DGRIAYEICP RTEEKKNDRI CTNCCAGTKG CKYFSYDGTF VCEGESDPRN PKACPRNCDG RIAYGICPRA 280
EEKNNQICTN CCAGMKGCNY FSANGTFICE GESEYVSKVD EYVLEVENDL QKSRVAVS 338
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSNVEYADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-81
171 - 222 52
IRD-227
113 - 164 52
IRD-228
229 - 280 52
IRD-271
55 - 106 52
IRD-535
30 - 80 51
IRD-590
262 - 311 50
IRD-651
204 - 254 51
IRD-652
146 - 196 51
IRD-674
88 - 138 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.394
3.7 -15.9 -30.4
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL41 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158191
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-17 CPRIC Trypsin 91 - 95
RL-17 CPRIC Trypsin 91 - 95
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-81 171 - 222 CPRNC 206 - 210 DPTNP 199 - 203 H-L Type (Type-I)
IRD-227 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-228 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-271 55 - 106 CPRIC 90 - 94 DPKNP 83 - 87 H-L Type (Type-I)
IRD-535 30 - 80 CPRNC 32 - 36 EKEDN 50 - 54 L-H Type (Type-II)
IRD-590 262 - 311 CPRNC 264 - 268 EEKNN 281 - 285 L-H Type (Type-II)
IRD-651 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-652 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-674 88 - 138 CPRIC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
RICTNCCAGTKGCKYFSYDGNFVCEGESDPTNPKACPRNCDGRIAYEICPRT
CPRNC
DPTNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S Y D G N F V C E G E S D P T N P K A C P R N C D G R I A Y E I C P R T


RICTNCCAGTKGCNYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYGICRRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C N Y F S D D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G I C R R S


RICTNCCAGTKGCKYFSYDGTFVCEGESDPRNPKACPRNCDGRIAYGICPRA
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S Y D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P R A


QICTNCCAGRKGCNYFSADGTFVCEGESDPKNPKGCPRICDPRIAYRICRRS
CPRIC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G R K G C N Y F S A D G T F V C E G E S D P K N P K G C P R I C D P R I A Y R I C R R S


KACPRNCDPRIAYGVCPRSEEKEDNQICTNCCAGRKGCNYFSADGTFVCEG
CPRNC
EKEDN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E K E D N Q I C T N C C A G R K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGICPRAEEKNNQICTNCCAGMKGCNYFSANGTFICEG
CPRNC
EEKNN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D G R I A Y G I C P R A E E K N N Q I C T N C C A G M K G C N Y F S A N G T F I C E G


KACPRNCDGRIAYEICPRTEEKKNDRICTNCCAGTKGCKYFSYDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R T E E K K N D R I C T N C C A G T K G C K Y F S Y D G T F V C E G


KACPRNCDPRIAYGICRRSEEKKNDRICTNCCAGTKGCKYFSYDGNFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C R R S E E K K N D R I C T N C C A G T K G C K Y F S Y D G N F V C E G


KGCPRICDPRIAYRICRRSEEKKNDRICTNCCAGTKGCNYFSDDGTFVCEG
CPRIC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C P R I C D P R I A Y R I C R R S E E K K N D R I C T N C C A G T K G C N Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-81
 171 - 222 52
IRD-227
 113 - 164 52
IRD-228
 229 - 280 52
IRD-271
 55 - 106 52
IRD-535
 30 - 80 51
IRD-590
 262 - 311 50
IRD-651
 204 - 254 51
IRD-652
 146 - 196 51
IRD-674
 88 - 138 51
Amino Acid Count % by Frequency
Alanine 22 6.51
Cysteine 40 11.83
Aspartic acid 21 6.21
Glutamic acid 27 7.99
Phenylalanine 12 3.55
Glycine 27 7.99
Histidine 1 0.3
Isoleucine 16 4.73
Lysine 23 6.8
Leucine 9 2.66
Methionine 3 0.89
Asparagine 26 7.69
Proline 19 5.62
Glutamine 3 0.89
Arginine 26 7.69
Serine 19 5.62
Threonine 14 4.14
Valine 15 4.44
Tryptophan 0 0
Tyrosine 15 4.44