Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_144 [Q1WL45]  : Trypsin proteinase inhibitor

394Length

Nicotiana repandaSpecie

PIGene Name

43028Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSNVEHADAC TFNCDPKIAY EVCPRSEEKT NNQICTNCCS GTKGCRFFSA 70
NGTFVCEGES DPRNPKACPL NCDPRIAIGI CPLSQEKKND QLCTNCCAGK KGCNYFSDDG TFICEGESDP 140
NNPKDCPRNC DGRIAYGICP LSEEKKNDRT CTNCCAGTKG CNYFSYDGTF VCEGESDPRN PKFCPQNCDG 210
RIAYGICPVS EEKKNNRLCT NCCAGAKGCR FFSADGTFVC EGESDPRNPK FCPLNCDPRI ASGICTLSEE 280
KKNDRLCTNC CAGTKGCRFF SADGTFVCEG ESDPRNPKAC PLNCDPRIAT GICPYSKEKK NDRICTNCCS 350
GKKGCKYFSD DGTFVCEGET EYPSKVDEYV HEVENDLQSK VAVS 394
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-77
111 - 162 52
IRD-244
53 - 104 52
IRD-245
227 - 278 52
IRD-246
285 - 336 52
IRD-270
169 - 220 52
IRD-441
86 - 136 51
IRD-462
28 - 78 51
IRD-492
318 - 368 51
IRD-642
144 - 194 51
IRD-657
260 - 310 51
IRD-673
202 - 252 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.025
-2 -26.3 -43.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL45 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158187
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-16 CPQNC Unknown 205 - 209
RL-16 CPQNC Unknown 205 - 209
RL-2 CPLNC Chymotrypsin 89 - 93
RL-2 CPLNC Chymotrypsin 89 - 93
RL-2 CPLNC Chymotrypsin 263 - 267
RL-2 CPLNC Chymotrypsin 263 - 267
RL-2 CPLNC Chymotrypsin 321 - 325
RL-2 CPLNC Chymotrypsin 321 - 325
RL-7 CTFNC Chymotrypsin 31 - 35
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-77 111 - 162 CPRNC 146 - 150 DPNNP 139 - 143 H-L Type (Type-I)
IRD-244 53 - 104 CPLNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-245 227 - 278 CPLNC 262 - 266 DPRNP 255 - 259 H-L Type (Type-I)
IRD-246 285 - 336 CPLNC 320 - 324 DPRNP 313 - 317 H-L Type (Type-I)
IRD-270 169 - 220 CPQNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-441 86 - 136 CPLNC 88 - 92 QEKKN 105 - 109 L-H Type (Type-II)
IRD-462 28 - 78 CTFNC 30 - 34 EEKTN 47 - 51 L-H Type (Type-II)
IRD-492 318 - 368 CPLNC 320 - 324 KEKKN 337 - 341 L-H Type (Type-II)
IRD-642 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-657 260 - 310 CPLNC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
IRD-673 202 - 252 CPQNC 204 - 208 EEKKN 221 - 225 L-H Type (Type-II)
QLCTNCCAGKKGCNYFSDDGTFICEGESDPNNPKDCPRNCDGRIAYGICPLS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q L C T N C C A G K K G C N Y F S D D G T F I C E G E S D P N N P K D C P R N C D G R I A Y G I C P L S


QICTNCCSGTKGCRFFSANGTFVCEGESDPRNPKACPLNCDPRIAIGICPLS
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C S G T K G C R F F S A N G T F V C E G E S D P R N P K A C P L N C D P R I A I G I C P L S


RLCTNCCAGAKGCRFFSADGTFVCEGESDPRNPKFCPLNCDPRIASGICTLS
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G A K G C R F F S A D G T F V C E G E S D P R N P K F C P L N C D P R I A S G I C T L S


RLCTNCCAGTKGCRFFSADGTFVCEGESDPRNPKACPLNCDPRIATGICPYS
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C R F F S A D G T F V C E G E S D P R N P K A C P L N C D P R I A T G I C P Y S


RTCTNCCAGTKGCNYFSYDGTFVCEGESDPRNPKFCPQNCDGRIAYGICPVS
CPQNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R T C T N C C A G T K G C N Y F S Y D G T F V C E G E S D P R N P K F C P Q N C D G R I A Y G I C P V S


KACPLNCDPRIAIGICPLSQEKKNDQLCTNCCAGKKGCNYFSDDGTFICEG
CPLNC
QEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D P R I A I G I C P L S Q E K K N D Q L C T N C C A G K K G C N Y F S D D G T F I C E G


DACTFNCDPKIAYEVCPRSEEKTNNQICTNCCSGTKGCRFFSANGTFVCEG
CTFNC
EEKTN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
D A C T F N C D P K I A Y E V C P R S E E K T N N Q I C T N C C S G T K G C R F F S A N G T F V C E G


KACPLNCDPRIATGICPYSKEKKNDRICTNCCSGKKGCKYFSDDGTFVCEG
CPLNC
KEKKN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D P R I A T G I C P Y S K E K K N D R I C T N C C S G K K G C K Y F S D D G T F V C E G


KDCPRNCDGRIAYGICPLSEEKKNDRTCTNCCAGTKGCNYFSYDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K D C P R N C D G R I A Y G I C P L S E E K K N D R T C T N C C A G T K G C N Y F S Y D G T F V C E G


KFCPLNCDPRIASGICTLSEEKKNDRLCTNCCAGTKGCRFFSADGTFVCEG
CPLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K F C P L N C D P R I A S G I C T L S E E K K N D R L C T N C C A G T K G C R F F S A D G T F V C E G


KFCPQNCDGRIAYGICPVSEEKKNNRLCTNCCAGAKGCRFFSADGTFVCEG
CPQNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K F C P Q N C D G R I A Y G I C P V S E E K K N N R L C T N C C A G A K G C R F F S A D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-77
 111 - 162 52
IRD-244
 53 - 104 52
IRD-245
 227 - 278 52
IRD-246
 285 - 336 52
IRD-270
 169 - 220 52
IRD-441
 86 - 136 51
IRD-462
 28 - 78 51
IRD-492
 318 - 368 51
IRD-642
 144 - 194 51
IRD-657
 260 - 310 51
IRD-673
 202 - 252 51
Amino Acid Count % by Frequency
Alanine 21 5.33
Cysteine 48 12.18
Aspartic acid 26 6.6
Glutamic acid 28 7.11
Phenylalanine 20 5.08
Glycine 32 8.12
Histidine 3 0.76
Isoleucine 15 3.81
Lysine 29 7.36
Leucine 17 4.31
Methionine 2 0.51
Asparagine 31 7.87
Proline 25 6.35
Glutamine 5 1.27
Arginine 19 4.82
Serine 26 6.6
Threonine 21 5.33
Valine 16 4.06
Tryptophan 0 0
Tyrosine 10 2.54