Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_152 [Q1WL36]  : Trypsin proteinase inhibitor

448Length

Nicotiana rusticaSpecie

PIGene Name

48643Mass

13Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLLGMSLL VSNVEHADAK ACTLNCDPRI AYGVCPRSEE KKNNQICTNC CAGTKGCNYF 70
SADGTFVCEG ESDPRNPKAC PRNCDPRIAY GVCPRTEEKK DNQICTNCCA GTKGCNYFSA NGTFICEGES 140
DPRNPKACTL NCDPRIAYGV CPRSEEKKNN QICTNCCAGT KGCNYFSADG TFVCKGESDP RNPKACPRNC 210
DPRIAYGVCP RTEEKKDNQI CTNCCAGTKG CNYFSANGTF ICEGESDPRN PKACTLNCDP RIAYGVCPRS 280
EEKKNNQICT NCCAGTKGCN YFSADGTFVC EGESDPRNPK ACPRNCDGRI AYGVCPRSEE KKNNRICTNC 350
CAGTKGCNYF SADGTFVCEG ESDPRNPKAC PRNCDGRIAY GVCPRSEEKK NNRICTNCCA GTKGCNYFSA 420
DGTFICEGES EYVNKVENDL EKSKVAVI 448
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLLGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-170
55 - 106 52
IRD-171
171 - 222 52
IRD-172
287 - 338 52
IRD-173
345 - 396 52
IRD-252
113 - 164 52
IRD-252
229 - 280 52
IRD-594
204 - 254 51
IRD-594
88 - 138 51
IRD-613
320 - 370 51
IRD-614
378 - 428 51
IRD-659
262 - 312 51
IRD-659
30 - 80 51
IRD-660
146 - 196 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.90799999999999
9.5 -17.7 -37.9
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL36 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158196
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 381 - 385
RL-1 CPRNC Trypsin 381 - 385
RL-3 CTLNC Chymotrypsin 33 - 37
RL-3 CTLNC Chymotrypsin 149 - 153
RL-3 CTLNC Chymotrypsin 149 - 153
RL-3 CTLNC Chymotrypsin 265 - 269
RL-3 CTLNC Chymotrypsin 265 - 269
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-170 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-171 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-172 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-173 345 - 396 CPRNC 380 - 384 DPRNP 373 - 377 H-L Type (Type-I)
IRD-252 113 - 164 CTLNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-252 229 - 280 CTLNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-594 204 - 254 CPRNC 206 - 210 EEKKD 223 - 227 L-H Type (Type-II)
IRD-594 88 - 138 CPRNC 90 - 94 EEKKD 107 - 111 L-H Type (Type-II)
IRD-613 320 - 370 CPRNC 322 - 326 EEKKN 339 - 343 L-H Type (Type-II)
IRD-614 378 - 428 CPRNC 380 - 384 EEKKN 397 - 401 L-H Type (Type-II)
IRD-659 262 - 312 CTLNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
IRD-659 30 - 80 CTLNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-660 146 - 196 CTLNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDPRIAYGVCPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G V C P R T


QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIAYGVCPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I A Y G V C P R T


QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDGRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G V C P R S


RICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDGRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G V C P R S


QICTNCCAGTKGCNYFSANGTFICEGESDPRNPKACTLNCDPRIAYGVCPRS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F I C E G E S D P R N P K A C T L N C D P R I A Y G V C P R S


QICTNCCAGTKGCNYFSANGTFICEGESDPRNPKACTLNCDPRIAYGVCPRS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F I C E G E S D P R N P K A C T L N C D P R I A Y G V C P R S


KACPRNCDPRIAYGVCPRTEEKKDNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R T E E K K D N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


KACPRNCDPRIAYGVCPRTEEKKDNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R T E E K K D N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


KACPRNCDGRIAYGVCPRSEEKKNNRICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R S E E K K N N R I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRSEEKKNNRICTNCCAGTKGCNYFSADGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R S E E K K N N R I C T N C C A G T K G C N Y F S A D G T F I C E G


KACTLNCDPRIAYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCKG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-170
 55 - 106 52
IRD-171
 171 - 222 52
IRD-172
 287 - 338 52
IRD-173
 345 - 396 52
IRD-252
 113 - 164 52
IRD-252
 229 - 280 52
IRD-594
 204 - 254 51
IRD-594
 88 - 138 51
IRD-613
 320 - 370 51
IRD-614
 378 - 428 51
IRD-659
 262 - 312 51
IRD-659
 30 - 80 51
IRD-660
 146 - 196 51
Amino Acid Count % by Frequency
Alanine 33 7.37
Cysteine 56 12.5
Aspartic acid 22 4.91
Glutamic acid 31 6.92
Phenylalanine 15 3.35
Glycine 38 8.48
Histidine 2 0.45
Isoleucine 18 4.02
Lysine 32 7.14
Leucine 12 2.68
Methionine 2 0.45
Asparagine 44 9.82
Proline 28 6.25
Glutamine 5 1.12
Arginine 27 6.03
Serine 23 5.13
Threonine 26 5.8
Valine 19 4.24
Tryptophan 0 0
Tyrosine 15 3.35