Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_154 [Q1WL50]  : Trypsin proteinase inhibitor

279Length

Nicotiana benthamianaSpecie

PIGene Name

30816Mass

7Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLFFGMSLL VSNVEHAKAC TRECDTRIAY GVCPRLEEKT NNRICTNCCA GKKGCKYFSD 70
DGTFVCEGES DPRNPKPCPR NCDGRIAYGI CPLSEEKKNN RLCTNCCAGT KGCNYFSADG TFVCEGESDP 140
RNPKACPRNC DPRIDYRVCP RSEEKKNDRI CTNCCSGKKG CKYFSDDGTF VCEGESDPRN PKGCTFECDP 210
RIAYGICPLS EEKKNNRLCT NCCAGKKGCN YFSSNGTFIC EGESEYGSKV VEYVGEVEDD LQKSKVAVS 279
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLFFGMSLLVSNVEHAKA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-211
53 - 104 52
IRD-230
111 - 162 52
IRD-261
169 - 220 52
IRD-449
28 - 78 51
IRD-640
86 - 136 51
IRD-654
144 - 194 51
IRD-669
202 - 252 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.781
5 -11.6 -23.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL50 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158182
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-4 CTREC Trypsin 31 - 35
RL-8 CTFEC Chymotrypsin 205 - 209
RL-8 CTFEC Chymotrypsin 205 - 209
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-211 53 - 104 CPRNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-230 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-261 169 - 220 CTFEC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-449 28 - 78 CTREC 30 - 34 EEKTN 47 - 51 L-H Type (Type-II)
IRD-640 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-654 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-669 202 - 252 CTFEC 204 - 208 EEKKN 221 - 225 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D P R I D Y R V C P R S


RICTNCCSGKKGCKYFSDDGTFVCEGESDPRNPKGCTFECDPRIAYGICPLS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C S G K K G C K Y F S D D G T F V C E G E S D P R N P K G C T F E C D P R I A Y G I C P L S


KACTRECDTRIAYGVCPRLEEKTNNRICTNCCAGKKGCKYFSDDGTFVCEG
CTREC
EEKTN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R E C D T R I A Y G V C P R L E E K T N N R I C T N C C A G K K G C K Y F S D D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDPRIDYRVCPRSEEKKNDRICTNCCSGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D Y R V C P R S E E K K N D R I C T N C C S G K K G C K Y F S D D G T F V C E G


KGCTFECDPRIAYGICPLSEEKKNNRLCTNCCAGKKGCNYFSSNGTFICEG
CTFEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T F E C D P R I A Y G I C P L S E E K K N N R L C T N C C A G K K G C N Y F S S N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-211
 53 - 104 52
IRD-230
 111 - 162 52
IRD-261
 169 - 220 52
IRD-449
 28 - 78 51
IRD-640
 86 - 136 51
IRD-654
 144 - 194 51
IRD-669
 202 - 252 51
Amino Acid Count % by Frequency
Alanine 13 4.66
Cysteine 32 11.47
Aspartic acid 16 5.73
Glutamic acid 23 8.24
Phenylalanine 12 4.3
Glycine 24 8.6
Histidine 2 0.72
Isoleucine 9 3.23
Lysine 23 8.24
Leucine 12 4.3
Methionine 2 0.72
Asparagine 20 7.17
Proline 15 5.38
Glutamine 1 0.36
Arginine 18 6.45
Serine 19 6.81
Threonine 13 4.66
Valine 15 5.38
Tryptophan 0 0
Tyrosine 10 3.58