Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_198 [Q1WL47]  : Trypsin proteinase inhibitor

330Length

Nicotiana simulansSpecie

PIGene Name

36019Mass

9Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLFFGMSLL VSNVEHAKAC TRECDTRIAY GVCPRLEEKT NNRICTNCCA GKKGCKYFSD 70
DGTFVCEGES DPRNPKPCPR NCDGRIAYGI CPLSEEKKNN QLCTNCCAGT KGCKYFSADG TFVCEGESDP 140
RNPKPCPRNC DGRIAYGICP LSEEKKNNRL CTNCCAGTKG CSYFSADGTF VCEGESDPRN PKPCPRNCDG 210
RIAYGICPRS EEKKNDRICT NCCSGKKGCK YFSDDGTFVC EGESDPRNPK GCTFECDPGI AYGICPLSEE 280
KKNNRLCTNC CAGKKGCNYF SSNGTFICEG ESEYASKVVE YVGEVEVAVS 330
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLFFGMSLLVSNVEHAKA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-211
53 - 104 52
IRD-217
169 - 220 52
IRD-218
111 - 162 52
IRD-259
227 - 278 52
IRD-449
28 - 78 51
IRD-644
144 - 194 51
IRD-648
86 - 136 51
IRD-649
202 - 252 51
IRD-670
260 - 310 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.629
5.2 -15 -29.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL47 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158185
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-4 CTREC Trypsin 31 - 35
RL-8 CTFEC Chymotrypsin 263 - 267
RL-8 CTFEC Chymotrypsin 263 - 267
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-211 53 - 104 CPRNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-217 169 - 220 CPRNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-218 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-259 227 - 278 CTFEC 262 - 266 DPRNP 255 - 259 H-L Type (Type-I)
IRD-449 28 - 78 CTREC 30 - 34 EEKTN 47 - 51 L-H Type (Type-II)
IRD-644 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-648 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-649 202 - 252 CPRNC 204 - 208 EEKKN 221 - 225 L-H Type (Type-II)
IRD-670 260 - 310 CTFEC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGTKGCSYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C S Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


QLCTNCCAGTKGCKYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q L C T N C C A G T K G C K Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RICTNCCSGKKGCKYFSDDGTFVCEGESDPRNPKGCTFECDPGIAYGICPLS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C S G K K G C K Y F S D D G T F V C E G E S D P R N P K G C T F E C D P G I A Y G I C P L S


KACTRECDTRIAYGVCPRLEEKTNNRICTNCCAGKKGCKYFSDDGTFVCEG
CTREC
EEKTN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R E C D T R I A Y G V C P R L E E K T N N R I C T N C C A G K K G C K Y F S D D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGTKGCSYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G T K G C S Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNQLCTNCCAGTKGCKYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N Q L C T N C C A G T K G C K Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPRSEEKKNDRICTNCCSGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C S G K K G C K Y F S D D G T F V C E G


KGCTFECDPGIAYGICPLSEEKKNNRLCTNCCAGKKGCNYFSSNGTFICEG
CTFEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T F E C D P G I A Y G I C P L S E E K K N N R L C T N C C A G K K G C N Y F S S N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-211
 53 - 104 52
IRD-217
 169 - 220 52
IRD-218
 111 - 162 52
IRD-259
 227 - 278 52
IRD-449
 28 - 78 51
IRD-644
 144 - 194 51
IRD-648
 86 - 136 51
IRD-649
 202 - 252 51
IRD-670
 260 - 310 51
Amino Acid Count % by Frequency
Alanine 17 5.15
Cysteine 40 12.12
Aspartic acid 16 4.85
Glutamic acid 27 8.18
Phenylalanine 14 4.24
Glycine 32 9.7
Histidine 2 0.61
Isoleucine 12 3.64
Lysine 26 7.88
Leucine 13 3.94
Methionine 2 0.61
Asparagine 24 7.27
Proline 20 6.06
Glutamine 1 0.3
Arginine 19 5.76
Serine 22 6.67
Threonine 16 4.85
Valine 15 4.55
Tryptophan 0 0
Tyrosine 12 3.64