Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_203 [Q1WL46]  : Trypsin proteinase inhibitor

339Length

Nicotiana umbraticaSpecie

PIGene Name

37309Mass

9Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLLGMSLL VSNVEHADAM TCTRECDPRI DYRVCPLSEE KKNDRICTNC CAGKKGCKYF 70
SDDGTFVCEG ESDPRNPKPC PRNCDGRIAY GICPLSEEKK NDPICTNCCV GKKGCKYFSY DGTFVCEGES 140
DPRNPKPCPR NCDGRIAYGI CPLSEEKKND PICTNCCAGK KGCKYFSYDG TFVCEGESDP RNPKPCPRNC 210
DGRIAYGICP LSEEKKNDRL CTNCCAGKKG CKYFSDDGTF VCEGESDPRN PKGCTFECDP RIAYGICPLS 280
EEKKNNRLCT NCCAGKKGCN YFSSNGTFIC EGESEYASKV VEYVGEVEID LPKSKVAVS 339
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLLGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-211
55 - 106 52
IRD-225
113 - 164 52
IRD-226
171 - 222 52
IRD-260
229 - 280 52
IRD-645
88 - 138 51
IRD-646
146 - 196 51
IRD-647
204 - 254 51
IRD-663
30 - 80 51
IRD-669
262 - 312 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.176
3.3 -17.1 -32
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL46 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158186
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-4 CTREC Trypsin 33 - 37
RL-8 CTFEC Chymotrypsin 265 - 269
RL-8 CTFEC Chymotrypsin 265 - 269
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-211 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-225 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-226 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-260 229 - 280 CTFEC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-645 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-646 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-647 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-663 30 - 80 CTREC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-669 262 - 312 CTFEC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


PICTNCCVGKKGCKYFSYDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,49 ; 7,36 ; 13,24
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C V G K K G C K Y F S Y D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


PICTNCCAGKKGCKYFSYDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G K K G C K Y F S Y D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKGCTFECDPRIAYGICPLS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K G C T F E C D P R I A Y G I C P L S


KPCPRNCDGRIAYGICPLSEEKKNDPICTNCCVGKKGCKYFSYDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N D P I C T N C C V G K K G C K Y F S Y D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNDPICTNCCAGKKGCKYFSYDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N D P I C T N C C A G K K G C K Y F S Y D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNDRLCTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N D R L C T N C C A G K K G C K Y F S D D G T F V C E G


MTCTRECDPRIDYRVCPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CTREC
EEKKN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
M T C T R E C D P R I D Y R V C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KGCTFECDPRIAYGICPLSEEKKNNRLCTNCCAGKKGCNYFSSNGTFICEG
CTFEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T F E C D P R I A Y G I C P L S E E K K N N R L C T N C C A G K K G C N Y F S S N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-211
 55 - 106 52
IRD-225
 113 - 164 52
IRD-226
 171 - 222 52
IRD-260
 229 - 280 52
IRD-645
 88 - 138 51
IRD-646
 146 - 196 51
IRD-647
 204 - 254 51
IRD-663
 30 - 80 51
IRD-669
 262 - 312 51
Amino Acid Count % by Frequency
Alanine 14 4.13
Cysteine 40 11.8
Aspartic acid 22 6.49
Glutamic acid 27 7.96
Phenylalanine 12 3.54
Glycine 30 8.85
Histidine 2 0.59
Isoleucine 14 4.13
Lysine 31 9.14
Leucine 16 4.72
Methionine 3 0.88
Asparagine 21 6.19
Proline 24 7.08
Glutamine 0 0
Arginine 18 5.31
Serine 22 6.49
Threonine 13 3.83
Valine 16 4.72
Tryptophan 0 0
Tyrosine 14 4.13