Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_247 [Q1WL48]  : Trypsin proteinase inhibitor

330Length

Nicotiana megalosiphonSpecie

PIGene Name

36033Mass

9Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLSFGMSLL VSNVEHAKAC TRECDTRIAY GVCPRLEEKT NNRICTNCCA GKKGCKYFSA 70
DGTFVCEGES DPRNPKPCPR NCDGRIAYGI CPLSEEKKNN QLCTNCCAGT KGCNYFSADG TFVCEGESDP 140
RNPKPCPRNC DGRIAYGICP LSEEKKNNRL CTNCCAGTKG CSYFSADGTF VCEGESDPRN PKPCPRNCDG 210
RIAYGICPRS EEKKSDRICT NCCSGKKGCK YFSDDGTFVC EGEFDPRNPK GCTFECDPRI AYGICPLSEE 280
KKNNRLCTNC CAGKKGCNYF SSNGTFICEG ESEYASKVVE YVGEVEVAVS 330
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLSFGMS
 1 ↔ 18
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-215
53 - 104 52
IRD-216
111 - 162 52
IRD-217
169 - 220 52
IRD-258
227 - 278 52
IRD-450
28 - 78 51
IRD-509
202 - 252 51
IRD-643
86 - 136 51
IRD-644
144 - 194 51
IRD-669
260 - 310 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.774
6.2 -14 -28.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL48 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158184
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-4 CTREC Trypsin 31 - 35
RL-8 CTFEC Chymotrypsin 263 - 267
RL-8 CTFEC Chymotrypsin 263 - 267
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-215 53 - 104 CPRNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-216 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-217 169 - 220 CPRNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-258 227 - 278 CTFEC 262 - 266 DPRNP 255 - 259 H-L Type (Type-I)
IRD-450 28 - 78 CTREC 30 - 34 EEKTN 47 - 51 L-H Type (Type-II)
IRD-509 202 - 252 CPRNC 204 - 208 EEKKS 221 - 225 L-H Type (Type-II)
IRD-643 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-644 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-669 260 - 310 CTFEC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
RICTNCCAGKKGCKYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


QLCTNCCAGTKGCNYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q L C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGTKGCSYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C S Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


RICTNCCSGKKGCKYFSDDGTFVCEGEFDPRNPKGCTFECDPRIAYGICPLS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C S G K K G C K Y F S D D G T F V C E G E F D P R N P K G C T F E C D P R I A Y G I C P L S


KACTRECDTRIAYGVCPRLEEKTNNRICTNCCAGKKGCKYFSADGTFVCEG
CTREC
EEKTN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R E C D T R I A Y G V C P R L E E K T N N R I C T N C C A G K K G C K Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPRSEEKKSDRICTNCCSGKKGCKYFSDDGTFVCEG
CPRNC
EEKKS
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K S D R I C T N C C S G K K G C K Y F S D D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNQLCTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N Q L C T N C C A G T K G C N Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGTKGCSYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G T K G C S Y F S A D G T F V C E G


KGCTFECDPRIAYGICPLSEEKKNNRLCTNCCAGKKGCNYFSSNGTFICEG
CTFEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T F E C D P R I A Y G I C P L S E E K K N N R L C T N C C A G K K G C N Y F S S N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-215
 53 - 104 52
IRD-216
 111 - 162 52
IRD-217
 169 - 220 52
IRD-258
 227 - 278 52
IRD-450
 28 - 78 51
IRD-509
 202 - 252 51
IRD-643
 86 - 136 51
IRD-644
 144 - 194 51
IRD-669
 260 - 310 51
Amino Acid Count % by Frequency
Alanine 18 5.45
Cysteine 40 12.12
Aspartic acid 15 4.55
Glutamic acid 27 8.18
Phenylalanine 14 4.24
Glycine 31 9.39
Histidine 2 0.61
Isoleucine 12 3.64
Lysine 25 7.58
Leucine 13 3.94
Methionine 2 0.61
Asparagine 24 7.27
Proline 20 6.06
Glutamine 1 0.3
Arginine 20 6.06
Serine 23 6.97
Threonine 16 4.85
Valine 15 4.55
Tryptophan 0 0
Tyrosine 12 3.64