Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_250 [Q84LJ9]  : 7-domain trypsin inhibitor (Trypsin proteinase inhibitor)

455Length

Nicotiana attenuataSpecie

7-domain TI PIGene Name

50513Mass

13Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSML VSNVEHADAK ACPRNCDGRI AYEICPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFICEG ESDPRNPKAC PRNCDGRIAY GICPRSEEKK NDRICTNCCA GTKGCKYFSD DGTFICEGES 140
DPRNPKACPR NCDGRIAYEI CPRSEEKKND RICTNCCTGT KGCKYFSDDG TFVCEGESDP RNPKACPRNC 210
DGRIAYEICP RSEEKKNDRI CTNCCAGTKG CKYFSDDGTF ICEGESDPRN PKACPRNCDG RIAYEICPRS 280
EEKKNDRICT NCCAGTKGCK YFSDDGTFIC EGESDPRNPK ACPRNCDGRI AYEICPRTEE KKNDRICTNC 350
CAGTKGCKYF SDDGTFVCEG ESDPRNPKAC PRNCDERIAY GICPRTEEKK NNQICTNCCA GTKGCNYFSA 420
NGTFICEGES EYVSKVDEYV HEVENDLQKS RVAVS 455
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSMLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-165
55 - 106 52
IRD-166
113 - 164 52
IRD-166
229 - 280 52
IRD-167
171 - 222 52
IRD-168
287 - 338 52
IRD-169
345 - 396 52
IRD-615
262 - 312 51
IRD-615
204 - 254 51
IRD-615
30 - 80 51
IRD-616
88 - 138 51
IRD-617
146 - 196 51
IRD-618
320 - 370 51
IRD-619
378 - 428 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.82299999999999
3 -25.8 -46.2
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q84LJ9 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF542547
DQ158200
REFERENCES
PubMed ID:     14976235    | 16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 381 - 385
RL-1 CPRNC Trypsin 381 - 385
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Manduca sexta; Tupiocoris notatus Evidence for the in planta defensive function of trypsin protease inhibitors 14976235
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-165 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-166 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-166 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-167 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-168 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-169 345 - 396 CPRNC 380 - 384 DPRNP 373 - 377 H-L Type (Type-I)
IRD-615 262 - 312 CPRNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
IRD-615 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-615 30 - 80 CPRNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-616 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-617 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-618 320 - 370 CPRNC 322 - 326 EEKKN 339 - 343 L-H Type (Type-II)
IRD-619 378 - 428 CPRNC 380 - 384 EEKKN 397 - 401 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R S


RICTNCCTGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C T G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y E I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R T


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDERIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D E R I A Y G I C P R T


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCTGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C T G T K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYEICPRTEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R T E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACPRNCDERIAYGICPRTEEKKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D E R I A Y G I C P R T E E K K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-165
 55 - 106 52
IRD-166
 113 - 164 52
IRD-166
 229 - 280 52
IRD-167
 171 - 222 52
IRD-168
 287 - 338 52
IRD-169
 345 - 396 52
IRD-615
 262 - 312 51
IRD-615
 204 - 254 51
IRD-615
 30 - 80 51
IRD-616
 88 - 138 51
IRD-617
 146 - 196 51
IRD-618
 320 - 370 51
IRD-619
 378 - 428 51
Amino Acid Count % by Frequency
Alanine 26 5.71
Cysteine 56 12.31
Aspartic acid 34 7.47
Glutamic acid 39 8.57
Phenylalanine 16 3.52
Glycine 37 8.13
Histidine 3 0.66
Isoleucine 26 5.71
Lysine 36 7.91
Leucine 7 1.54
Methionine 3 0.66
Asparagine 32 7.03
Proline 26 5.71
Glutamine 2 0.44
Arginine 35 7.69
Serine 25 5.49
Threonine 24 5.27
Valine 12 2.64
Tryptophan 0 0
Tyrosine 16 3.52