Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_254 [Q1WL30]  : Trypsin proteinase inhibitor

389Length

Nicotiana quadrivalvisSpecie

PIGene Name

42110Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSIVEHADAK ACPRNCDGRI AYGICPRSEE KKNDRICTNC CAGMKGCNYF 70
SADGTFVCEG ESDPRNPKAC PRNCDGRIAY GICPRSEEKK NDRICTNCCA GTKGCSYFSA DGTFVCKGES 140
DPRNPKACPR NCDPRIAYGA CPLSEEKKDN QICTNCCAGT KGCSYFSADG TFVCKGESDP RNPKACPRNC 210
DPRIAYGACP LSEEKKDNQI CTNCCAGTKG CSYFSADGTF VCKGESDPRN PKACPRNCDP RIDFGICPLS 280
EEKDNQICTN CCAGTKGCSY FSADGTFVCK GESDPRNPKA CPRNCDPRIA YGACPLSEEK KNNQICTNCC 350
AGMKGCNYFS ANGTFICEGE SEYVNKVEND LQKSMVAVF 389
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSIVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-185
55 - 106 52
IRD-186
113 - 164 52
IRD-189
171 - 222 52
IRD-189
286 - 337 52
IRD-190
229 - 280 52
IRD-575
262 - 311 50
IRD-600
146 - 196 51
IRD-600
204 - 254 51
IRD-607
30 - 80 51
IRD-637
88 - 138 51
IRD-638
319 - 369 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.809
7 -16.4 -33.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL30 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158198
DQ158202
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 322 - 326
RL-1 CPRNC Trypsin 322 - 326
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-185 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-186 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-189 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-189 286 - 337 CPRNC 321 - 325 DPRNP 314 - 318 H-L Type (Type-I)
IRD-190 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-575 262 - 311 CPRNC 264 - 268 EEKDN 281 - 285 L-H Type (Type-II)
IRD-600 146 - 196 CPRNC 148 - 152 EEKKD 165 - 169 L-H Type (Type-II)
IRD-600 204 - 254 CPRNC 206 - 210 EEKKD 223 - 227 L-H Type (Type-II)
IRD-607 30 - 80 CPRNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-637 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-638 319 - 369 CPRNC 321 - 325 EEKKN 338 - 342 L-H Type (Type-II)
RICTNCCAGMKGCNYFSADGTFVCEGESDPRNPKACPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIAYGACPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I A Y G A C P L S


QICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIAYGACPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I A Y G A C P L S


QICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIAYGACPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I A Y G A C P L S


QICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L S


KACPRNCDPRIDFGICPLSEEKDNQICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D F G I C P L S E E K D N Q I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPRNCDPRIAYGACPLSEEKKDNQICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G A C P L S E E K K D N Q I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPRNCDPRIAYGACPLSEEKKDNQICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G A C P L S E E K K D N Q I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPRNCDPRIAYGACPLSEEKKNNQICTNCCAGMKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G A C P L S E E K K N N Q I C T N C C A G M K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-185
 55 - 106 52
IRD-186
 113 - 164 52
IRD-189
 171 - 222 52
IRD-189
 286 - 337 52
IRD-190
 229 - 280 52
IRD-575
 262 - 311 50
IRD-600
 146 - 196 51
IRD-600
 204 - 254 51
IRD-607
 30 - 80 51
IRD-637
 88 - 138 51
IRD-638
 319 - 369 51
Amino Acid Count % by Frequency
Alanine 31 7.97
Cysteine 48 12.34
Aspartic acid 24 6.17
Glutamic acid 23 5.91
Phenylalanine 16 4.11
Glycine 33 8.48
Histidine 2 0.51
Isoleucine 17 4.37
Lysine 29 7.46
Leucine 12 3.08
Methionine 5 1.29
Asparagine 29 7.46
Proline 26 6.68
Glutamine 5 1.29
Arginine 22 5.66
Serine 26 6.68
Threonine 16 4.11
Valine 13 3.34
Tryptophan 0 0
Tyrosine 12 3.08