Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_260 [E9JGS8]  : Serine proteinase inhibitor 2a (Fragment)

147Length

Solanum nigrumSpecie

SPI2aGene Name

16195Mass

3Domain Count

Sequence :
     FASTA
AVHKVSFLAC LLVLGWMFLL AKHVDAKACT RECGHFSYGI CPRSEGSPEK PICTNCCSGY KGCNYYSAKG 70
DLICEGESDP RNPNDCTFEC DTQIAYSKCP RSEGKMIIKP TGCTTCCTGY QGCYYFDQDG DFVCEGESPE 140
PKTTAYY 147
SIGNAL PEPTIDE
SEQUENCE Position
AVHKVSFLACLLVLGWMFLLAKHVDA
 1 ↔ 26
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-264
51 - 102 52
IRD-409
84 - 136 53
IRD-458
27 - 76 50
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.952
-1.5 -11.2 -17.4
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
E9JGS8 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 GU133370
GU133371
REFERENCES
PubMed ID:     21177479    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-4 CTREC Trypsin 30 - 34
RL-8 CTFEC Chymotrypsin 87 - 91
RL-8 CTFEC Chymotrypsin 87 - 91
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves,Flowers Wounding, Oral secretion Oral secretion,Methyl Jasmonate Manduca sexta 21177479
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-264 51 - 102 CTFEC 86 - 90 DPRNP 79 - 83 H-L Type (Type-I)
IRD-409 84 - 136 CTFEC 86 - 90 - H+L Type (Type-III)
IRD-458 27 - 76 CTREC 29 - 33 EGSPE 45 - 49 L-H Type (Type-II)
PICTNCCSGYKGCNYYSAKGDLICEGESDPRNPNDCTFECDTQIAYSKCPRS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C S G Y K G C N Y Y S A K G D L I C E G E S D P R N P N D C T F E C D T Q I A Y S K C P R S


NDCTFECDTQIAYSKCPRSEGKMIIKPTGCTTCCTGYQGCYYFDQDGDFVCEG
CTFEC
H+L Type (Type-III)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N D C T F E C D T Q I A Y S K C P R S E G K M I I K P T G C T T C C T G Y Q G C Y Y F D Q D G D F V C E G


KACTRECGHFSYGICPRSEGSPEKPICTNCCSGYKGCNYYSAKGDLICEG
CTREC
EGSPE
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T R E C G H F S Y G I C P R S E G S P E K P I C T N C C S G Y K G C N Y Y S A K G D L I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-264
 51 - 102 52
IRD-409
 84 - 136 53
IRD-458
 27 - 76 50
Amino Acid Count % by Frequency
Alanine 8 5.44
Cysteine 17 11.56
Aspartic acid 8 5.44
Glutamic acid 10 6.8
Phenylalanine 6 4.08
Glycine 14 9.52
Histidine 3 2.04
Isoleucine 6 4.08
Lysine 10 6.8
Leucine 7 4.76
Methionine 2 1.36
Asparagine 4 2.72
Proline 9 6.12
Glutamine 3 2.04
Arginine 4 2.72
Serine 10 6.8
Threonine 10 6.8
Valine 5 3.4
Tryptophan 1 0.68
Tyrosine 10 6.8