Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_265 [A0A1S3YZT7]  : wound-induced proteinase inhibitor 2-like

396Length

Nicotiana tabacumSpecie

LOC107781430Gene Name

43227Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSNVEHAHAK ACPFNCDPRI AYGVCPRLKE KKNNQICTNC CAGTKGCNYF 70
STDGTFICKG ESDPRNPKAC PRNCDPRIAY GLCPLSKEKD NRICINCCAG TKGCNYFSAD GTFVCKGESD 140
PRNPKVCPLN CDPRIAYGLC PLSEEKKDNQ ICTNCCAGTK GCNYFSANGT FVCKGESDPR NPKACPRNCD 210
PRIAYGLCPL SEEKKDNRIC TNCCAGKKGC NYFSADGTFV CKGESDPRNP KVCPLNCDPR IAYGLCPLSE 280
EKKDNQICTN CCAGTKGCNY FSADGTFVCK GESDPRNPKA CPRNCDPRIA YGVCPRSEEK KNNQICTNCC 350
AGMKGCNYFS ANGTFICEGE SEYVSKVDEY VREVENDLQK SKVAVS 396
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSNVEHAHA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-203
55 - 106 52
IRD-204
170 - 221 52
IRD-205
286 - 337 52
IRD-242
112 - 163 52
IRD-243
228 - 279 52
IRD-501
30 - 80 51
IRD-532
88 - 137 50
IRD-599
203 - 253 51
IRD-602
145 - 195 51
IRD-603
261 - 311 51
IRD-632
319 - 369 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.273
14.5 -9.4 -27.2
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1S3YZT7 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 206 - 210
RL-1 CPRNC Trypsin 206 - 210
RL-1 CPRNC Trypsin 322 - 326
RL-1 CPRNC Trypsin 322 - 326
RL-2 CPLNC Chymotrypsin 148 - 152
RL-2 CPLNC Chymotrypsin 148 - 152
RL-2 CPLNC Chymotrypsin 264 - 268
RL-2 CPLNC Chymotrypsin 264 - 268
RL-5 CPFNC Chymotrypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-203 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-204 170 - 221 CPRNC 205 - 209 DPRNP 198 - 202 H-L Type (Type-I)
IRD-205 286 - 337 CPRNC 321 - 325 DPRNP 314 - 318 H-L Type (Type-I)
IRD-242 112 - 163 CPLNC 147 - 151 DPRNP 140 - 144 H-L Type (Type-I)
IRD-243 228 - 279 CPLNC 263 - 267 DPRNP 256 - 260 H-L Type (Type-I)
IRD-501 30 - 80 CPFNC 32 - 36 KEKKN 49 - 53 L-H Type (Type-II)
IRD-532 88 - 137 CPRNC 90 - 94 KEKDN 107 - 111 L-H Type (Type-II)
IRD-599 203 - 253 CPRNC 205 - 209 EEKKD 222 - 226 L-H Type (Type-II)
IRD-602 145 - 195 CPLNC 147 - 151 EEKKD 164 - 168 L-H Type (Type-II)
IRD-603 261 - 311 CPLNC 263 - 267 EEKKD 280 - 284 L-H Type (Type-II)
IRD-632 319 - 369 CPRNC 321 - 325 EEKKN 338 - 342 L-H Type (Type-II)
QICTNCCAGTKGCNYFSTDGTFICKGESDPRNPKACPRNCDPRIAYGLCPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S T D G T F I C K G E S D P R N P K A C P R N C D P R I A Y G L C P L S


QICTNCCAGTKGCNYFSANGTFVCKGESDPRNPKACPRNCDPRIAYGLCPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F V C K G E S D P R N P K A C P R N C D P R I A Y G L C P L S


QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I A Y G V C P R S


RICINCCAGTKGCNYFSADGTFVCKGESDPRNPKVCPLNCDPRIAYGLCPLS
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C I N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K V C P L N C D P R I A Y G L C P L S


RICTNCCAGKKGCNYFSADGTFVCKGESDPRNPKVCPLNCDPRIAYGLCPLS
CPLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C N Y F S A D G T F V C K G E S D P R N P K V C P L N C D P R I A Y G L C P L S


KACPFNCDPRIAYGVCPRLKEKKNNQICTNCCAGTKGCNYFSTDGTFICKG
CPFNC
KEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R L K E K K N N Q I C T N C C A G T K G C N Y F S T D G T F I C K G


KACPRNCDPRIAYGLCPLSKEKDNRICINCCAGTKGCNYFSADGTFVCKG
CPRNC
KEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I A Y G L C P L S K E K D N R I C I N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGLCPLSEEKKDNRICTNCCAGKKGCNYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G L C P L S E E K K D N R I C T N C C A G K K G C N Y F S A D G T F V C K G


KVCPLNCDPRIAYGLCPLSEEKKDNQICTNCCAGTKGCNYFSANGTFVCKG
CPLNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K V C P L N C D P R I A Y G L C P L S E E K K D N Q I C T N C C A G T K G C N Y F S A N G T F V C K G


KVCPLNCDPRIAYGLCPLSEEKKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPLNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K V C P L N C D P R I A Y G L C P L S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGVCPRSEEKKNNQICTNCCAGMKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G M K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-203
 55 - 106 52
IRD-204
 170 - 221 52
IRD-205
 286 - 337 52
IRD-242
 112 - 163 52
IRD-243
 228 - 279 52
IRD-501
 30 - 80 51
IRD-532
 88 - 137 50
IRD-599
 203 - 253 51
IRD-602
 145 - 195 51
IRD-603
 261 - 311 51
IRD-632
 319 - 369 51
Amino Acid Count % by Frequency
Alanine 26 6.57
Cysteine 48 12.12
Aspartic acid 21 5.3
Glutamic acid 22 5.56
Phenylalanine 15 3.79
Glycine 31 7.83
Histidine 3 0.76
Isoleucine 15 3.79
Lysine 34 8.59
Leucine 19 4.8
Methionine 3 0.76
Asparagine 35 8.84
Proline 28 7.07
Glutamine 5 1.26
Arginine 20 5.05
Serine 23 5.81
Threonine 16 4.04
Valine 18 4.55
Tryptophan 0 0
Tyrosine 14 3.54