Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_270 [Q9SQ77]  : Proteinase inhibitor

281Length

Nicotiana alataSpecie

-Gene Name

30737Mass

7Domain Count

Sequence :
     FASTA
MAAHRVSFLA LLLLFGMSLL VSNVEHADAK ACTLNCDPRI AYGVCPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFVCEG ESDPRNPKAC TLNCDPRIAY GVCPRSEEKK NDRICTNCCA GTKGCKYFSD DGTFVCEGES 140
DPKNPKACPR NCDPRIAYGI CPLSEEKKND RICTNCCAGK KGCKYFSDDG TFVCEGESDP RNPKACPRNC 210
DGRIAYGICP LSEEKKNDRI CTNCCAGKKG CKYFSDDGTF ICEGESEYAS KVDEYVGEVE NDLQKSKVAV 280
S 281
SIGNAL PEPTIDE
SEQUENCE Position
MAAHRVSFLALLLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-153
171 - 222 52
IRD-229
113 - 164 52
IRD-253
55 - 106 52
IRD-606
204 - 254 51
IRD-653
146 - 196 51
IRD-661
30 - 80 51
IRD-661
88 - 138 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.599
1 -15.7 -27.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q9SQ77 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF105340
REFERENCES
PubMed ID:     10794532    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-3 CTLNC Chymotrypsin 33 - 37
RL-3 CTLNC Chymotrypsin 91 - 95
RL-3 CTLNC Chymotrypsin 91 - 95
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
stigma; leaves 10794532
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-153 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-229 113 - 164 CPRNC 148 - 152 DPKNP 141 - 145 H-L Type (Type-I)
IRD-253 55 - 106 CTLNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-606 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-653 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-661 30 - 80 CTLNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-661 88 - 138 CTLNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPKNPKACPRNCDPRIAYGICPLS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P K N P K A C P R N C D P R I A Y G I C P L S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACTLNCDPRIAYGVCPRS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C T L N C D P R I A Y G V C P R S


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F I C E G


KACPRNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-153
 171 - 222 52
IRD-229
 113 - 164 52
IRD-253
 55 - 106 52
IRD-606
 204 - 254 51
IRD-653
 146 - 196 51
IRD-661
 30 - 80 51
IRD-661
 88 - 138 51
Amino Acid Count % by Frequency
Alanine 19 6.76
Cysteine 32 11.39
Aspartic acid 22 7.83
Glutamic acid 21 7.47
Phenylalanine 10 3.56
Glycine 23 8.19
Histidine 2 0.71
Isoleucine 11 3.91
Lysine 26 9.25
Leucine 12 4.27
Methionine 2 0.71
Asparagine 17 6.05
Proline 15 5.34
Glutamine 1 0.36
Arginine 15 5.34
Serine 18 6.41
Threonine 12 4.27
Valine 13 4.63
Tryptophan 0 0
Tyrosine 10 3.56