Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_271 [Q8W2K1]  : Proteinase inhibitor IIb

152Length

Solanum americanumSpecie

PIN2bGene Name

16413Mass

3Domain Count

Sequence :
     FASTA
MAVHKEVSSL AYLLVLGLMF LHVSAVKHVD AKPCTRECGN LGYGICPRSE GSPENPICTN CCSGYKGCNY 70
YSANGTFICE GSSDPKNPNT CPLFCDGDIA YSKCPRSEGE TIIYPTGCTT CCTGYKGCYY FSKEGEFVCE 140
GESDEPNVIS NQ 152
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKEVSSLAYLLVLGLMFLHVSA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-277
56 - 107 52
IRD-436
89 - 141 53
IRD-455
32 - 81 50
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.556
-3.4 -12.7 -18.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q8W2K1 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF209709
REFERENCES
PubMed ID:     11785934    | 16926166    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-35 CPLFC Chymotrypsin 92 - 96
RL-35 CPLFC Chymotrypsin 92 - 96
RL-4 CTREC Trypsin 35 - 39
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-277 56 - 107 CPLFC 91 - 95 DPKNP 84 - 88 H-L Type (Type-I)
IRD-436 89 - 141 CPLFC 91 - 95 EGETI 108 - 112 L-H Type (Type-II)
IRD-455 32 - 81 CTREC 34 - 38 EGSPE 50 - 54 L-H Type (Type-II)
PICTNCCSGYKGCNYYSANGTFICEGSSDPKNPNTCPLFCDGDIAYSKCPRS
CPLFC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C S G Y K G C N Y Y S A N G T F I C E G S S D P K N P N T C P L F C D G D I A Y S K C P R S


NTCPLFCDGDIAYSKCPRSEGETIIYPTGCTTCCTGYKGCYYFSKEGEFVCEG
CPLFC
EGETI
L-H Type (Type-II)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N T C P L F C D G D I A Y S K C P R S E G E T I I Y P T G C T T C C T G Y K G C Y Y F S K E G E F V C E G


KPCTRECGNLGYGICPRSEGSPENPICTNCCSGYKGCNYYSANGTFICEG
CTREC
EGSPE
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K P C T R E C G N L G Y G I C P R S E G S P E N P I C T N C C S G Y K G C N Y Y S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-277
 56 - 107 52
IRD-436
 89 - 141 53
IRD-455
 32 - 81 50
Amino Acid Count % by Frequency
Alanine 6 3.95
Cysteine 16 10.53
Aspartic acid 5 3.29
Glutamic acid 12 7.89
Phenylalanine 5 3.29
Glycine 16 10.53
Histidine 3 1.97
Isoleucine 7 4.61
Lysine 8 5.26
Leucine 8 5.26
Methionine 2 1.32
Asparagine 9 5.92
Proline 10 6.58
Glutamine 1 0.66
Arginine 3 1.97
Serine 14 9.21
Threonine 9 5.92
Valine 8 5.26
Tryptophan 0 0
Tyrosine 10 6.58