Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_277 [Q1WL38]  : Trypsin proteinase inhibitor

432Length

Nicotiana acuminataSpecie

PIGene Name

47837Mass

13Domain Count

Sequence :
     FASTA
MAVHRVSFLA FLLLFGTSLL VSNVEHADAK ACPFNCDPRI AYGVCPRSEE KKNNQICTNC CAGTKGCNYF 70
SADGTFVCKG ESDPRNPKAC PRNCDPRIDY RVCPRSEEKD NQMCTNCCAG TKGCNYFSAD GTFVCKGESD 140
PRNPKACPRN CDPRIDYRVC PRSEEKDNQI CTNCCAGTKG CNYFSVDGTF VCKGESDPRN PKACPRNCDP 210
RIDYRVCPRS EEKDNQICTN CCAGTKGCNY FSANGTFVCK GESDPRNPKA CPRNCDPRID YRVCPRSEEK 280
DNQICTNCCA GTKGCNYFSA DGTFVCKGES DPRNPKGCPR NCDPRIDYRV CPRSEEKNNQ ICTNCCAGTK 350
GCNYFSADGT FVCKGESDPR NPKACPRNCD PRIDYRVCWH SEEKNNQICT NCCAGTKGCN YFSANGTFIC 420
EGESEYVSKV DE 432
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLAFLLLFGTSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-176
55 - 106 52
IRD-179
112 - 163 52
IRD-180
169 - 220 52
IRD-181
226 - 277 52
IRD-182
283 - 334 52
IRD-183
340 - 391 52
IRD-569
88 - 137 50
IRD-570
145 - 194 50
IRD-571
202 - 251 50
IRD-572
259 - 308 50
IRD-584
316 - 365 50
IRD-585
373 - 422 50
IRD-666
30 - 80 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.927
9.8 -17.6 -37.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL38 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158194
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 148 - 152
RL-1 CPRNC Trypsin 148 - 152
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 262 - 266
RL-1 CPRNC Trypsin 262 - 266
RL-1 CPRNC Trypsin 319 - 323
RL-1 CPRNC Trypsin 319 - 323
RL-1 CPRNC Trypsin 376 - 380
RL-1 CPRNC Trypsin 376 - 380
RL-5 CPFNC Chymotrypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-176 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-179 112 - 163 CPRNC 147 - 151 DPRNP 140 - 144 H-L Type (Type-I)
IRD-180 169 - 220 CPRNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-181 226 - 277 CPRNC 261 - 265 DPRNP 254 - 258 H-L Type (Type-I)
IRD-182 283 - 334 CPRNC 318 - 322 DPRNP 311 - 315 H-L Type (Type-I)
IRD-183 340 - 391 CPRNC 375 - 379 DPRNP 368 - 372 H-L Type (Type-I)
IRD-569 88 - 137 CPRNC 90 - 94 EEKDN 107 - 111 L-H Type (Type-II)
IRD-570 145 - 194 CPRNC 147 - 151 EEKDN 164 - 168 L-H Type (Type-II)
IRD-571 202 - 251 CPRNC 204 - 208 EEKDN 221 - 225 L-H Type (Type-II)
IRD-572 259 - 308 CPRNC 261 - 265 EEKDN 278 - 282 L-H Type (Type-II)
IRD-584 316 - 365 CPRNC 318 - 322 EEKNN 335 - 339 L-H Type (Type-II)
IRD-585 373 - 422 CPRNC 375 - 379 EEKNN 392 - 396 L-H Type (Type-II)
IRD-666 30 - 80 CPFNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C P R S


QMCTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q M C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C P R S


QICTNCCAGTKGCNYFSVDGTFVCKGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S V D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C P R S


QICTNCCAGTKGCNYFSANGTFVCKGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKGCPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K G C P R N C D P R I D Y R V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIDYRVCWHS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C W H S


KACPRNCDPRIDYRVCPRSEEKDNQMCTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E K D N Q M C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIDYRVCPRSEEKDNQICTNCCAGTKGCNYFSVDGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E K D N Q I C T N C C A G T K G C N Y F S V D G T F V C K G


KACPRNCDPRIDYRVCPRSEEKDNQICTNCCAGTKGCNYFSANGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E K D N Q I C T N C C A G T K G C N Y F S A N G T F V C K G


KACPRNCDPRIDYRVCPRSEEKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KGCPRNCDPRIDYRVCPRSEEKNNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKNN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K G C P R N C D P R I D Y R V C P R S E E K N N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIDYRVCWHSEEKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKNN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C W H S E E K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


KACPFNCDPRIAYGVCPRSEEKKNNQICTNCCAGTKGCNYFSADGTFVCKG
CPFNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-176
 55 - 106 52
IRD-179
 112 - 163 52
IRD-180
 169 - 220 52
IRD-181
 226 - 277 52
IRD-182
 283 - 334 52
IRD-183
 340 - 391 52
IRD-569
 88 - 137 50
IRD-570
 145 - 194 50
IRD-571
 202 - 251 50
IRD-572
 259 - 308 50
IRD-584
 316 - 365 50
IRD-585
 373 - 422 50
IRD-666
 30 - 80 51
Amino Acid Count % by Frequency
Alanine 24 5.56
Cysteine 56 12.96
Aspartic acid 30 6.94
Glutamic acid 25 5.79
Phenylalanine 18 4.17
Glycine 31 7.18
Histidine 3 0.69
Isoleucine 14 3.24
Lysine 29 6.71
Leucine 6 1.39
Methionine 2 0.46
Asparagine 40 9.26
Proline 32 7.41
Glutamine 7 1.62
Arginine 32 7.41
Serine 25 5.79
Threonine 22 5.09
Valine 20 4.63
Tryptophan 1 0.23
Tyrosine 15 3.47