Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_279 [A0A1S3Z2Q3]  : wound-induced proteinase inhibitor 2-like

294Length

Nicotiana tabacumSpecie

LOC107782347Gene Name

31843Mass

6Domain Count

Sequence :
     FASTA
MAVHKVGFLS LLLLFGTFLL LSEVDYADAK ACTRNCNPRI RYGRCPKSGN KKFNVGCTNC CAGSLGCNYY 70
SANGTFVCEG ESETKVETIE TDVETIETEV ETMVESNKVC PRNCDPKIRY GRCPKSGNKK FNVGCTNCCA 140
AKKGCNYFSA NGTFVCEGET ETKVETIETE VETMVELNKV CPFNCDGRIS YSVCGNKKSS GRICTNCCAA 210
RKGCNYFSAD GTFVCKGESV VESMIEAVKP CRRNCDGRIS YSVCGNKKSS GRICTNCCAG KKGCNYFSAD 280
GTFVCEGESG VESI 294
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVGFLSLLLLFGTFLLLSEVDYADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-6
133 - 192 60
IRD-38
202 - 257 56
IRD-402
179 - 227 49
IRD-435
239 - 287 49
IRD-514
108 - 158 51
IRD-529
30 - 80 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.085
7.4 -8.7 -20.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1S3Z2Q3 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 111 - 115
RL-30 CTRNC Trypsin 33 - 37
RL-32 CRRNC Trypsin 242 - 246
RL-32 CRRNC Trypsin 242 - 246
RL-5 CPFNC Chymotrypsin 182 - 186
RL-5 CPFNC Chymotrypsin 182 - 186
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-6 133 - 192 CPFNC 181 - 185 - H+L Type (Type-III)
IRD-38 202 - 257 CRRNC 241 - 245 - H+L Type (Type-III)
IRD-402 179 - 227 CPFNC 181 - 185 - H+L Type (Type-III)
IRD-435 239 - 287 CRRNC 241 - 245 - H+L Type (Type-III)
IRD-514 108 - 158 CPRNC 110 - 114 GNKKF 127 - 131 L-H Type (Type-II)
IRD-529 30 - 80 CTRNC 32 - 36 GNKKF 49 - 53 L-H Type (Type-II)
VGCTNCCAAKKGCNYFSANGTFVCEGETETKVETIETEVETMVELNKVCPFNCDGRISYS
CPFNC
H+L Type (Type-III)
7,49 ; 24,53
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
V G C T N C C A A K K G C N Y F S A N G T F V C E G E T E T K V E T I E T E V E T M V E L N K V C P F N C D G R I S Y S


RICTNCCAARKGCNYFSADGTFVCKGESVVESMIEAVKPCRRNCDGRISYSVCGNK
CRRNC
H+L Type (Type-III)
3,44 ; 6,24 ; 7,40 ; 13,53
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56
R I C T N C C A A R K G C N Y F S A D G T F V C K G E S V V E S M I E A V K P C R R N C D G R I S Y S V C G N K


KVCPFNCDGRISYSVCGNKKSSGRICTNCCAARKGCNYFSADGTFVCKG
CPFNC
H+L Type (Type-III)
3,26 ; 7,30 ; 16,36 ; 29,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
K V C P F N C D G R I S Y S V C G N K K S S G R I C T N C C A A R K G C N Y F S A D G T F V C K G


KPCRRNCDGRISYSVCGNKKSSGRICTNCCAGKKGCNYFSADGTFVCEG
CRRNC
H+L Type (Type-III)
3,26 ; 7,30 ; 16,36 ; 29,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
K P C R R N C D G R I S Y S V C G N K K S S G R I C T N C C A G K K G C N Y F S A D G T F V C E G


KVCPRNCDPKIRYGRCPKSGNKKFNVGCTNCCAAKKGCNYFSANGTFVCEG
CPRNC
GNKKF
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K V C P R N C D P K I R Y G R C P K S G N K K F N V G C T N C C A A K K G C N Y F S A N G T F V C E G


KACTRNCNPRIRYGRCPKSGNKKFNVGCTNCCAGSLGCNYYSANGTFVCEG
CTRNC
GNKKF
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R N C N P R I R Y G R C P K S G N K K F N V G C T N C C A G S L G C N Y Y S A N G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-6
 133 - 192 60
IRD-38
 202 - 257 56
IRD-402
 179 - 227 49
IRD-435
 239 - 287 49
IRD-514
 108 - 158 51
IRD-529
 30 - 80 51
Amino Acid Count % by Frequency
Alanine 15 5.1
Cysteine 32 10.88
Aspartic acid 8 2.72
Glutamic acid 25 8.5
Phenylalanine 13 4.42
Glycine 29 9.86
Histidine 1 0.34
Isoleucine 11 3.74
Lysine 24 8.16
Leucine 10 3.4
Methionine 4 1.36
Asparagine 23 7.82
Proline 7 2.38
Glutamine 0 0
Arginine 14 4.76
Serine 23 7.82
Threonine 21 7.14
Valine 24 8.16
Tryptophan 0 0
Tyrosine 10 3.4