Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_28 [Q4ZIQ5]  : Pin-II type proteinase inhibitor 32 (Pin-II type proteinase inhibitor 7)

262Length

Capsicum annuumSpecie

PI-7Gene Name

28521Mass

7Domain Count

Sequence :
     FASTA
MAVPKEVSFL ASLLVLGILL LHVDAKACSQ RNAKEPICTN CCAGRKGCNY YSADGTFICE GESDPNNPKP 70
CTLNCDPRIF YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKPCP RNCDTRIAYS 140
KCPRSEGNAE NRICTNCCAG RKGCNYYSAD GTFICEGESD PNNPKPCTLN CDPRIFYSKC PRSEASAEQP 210
ICTNCCAGLK GCNYYNADGT FICEGESDPN HPKACPKNCD PNIAYSLCLY EK 262
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLASLLVLGILLLHVDA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-78
94 - 145 52
IRD-121
36 - 87 52
IRD-122
152 - 203 52
IRD-143
210 - 261 52
IRD-444
185 - 235 51
IRD-540
127 - 177 51
IRD-551
69 - 119 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.79299999999999
1.4 -14.9 -26.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q4ZIQ5 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ005913
JX106482
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-11 CPKNC Trypsin 246 - 250
RL-3 CTLNC Chymotrypsin 72 - 76
RL-3 CTLNC Chymotrypsin 72 - 76
RL-3 CTLNC Chymotrypsin 188 - 192
RL-3 CTLNC Chymotrypsin 188 - 192
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves Helicoverpa armigera Show 60-80% reduction in protease activity,Show activity for trypsin and chymotrypsin, 35% reduction in larval weight 15715970
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
Fruits Helicoverpa armigera Inhibition of 70% of the gut proteinase activity of Helicoverpa armigera,Larval and pupal growth reduction 17870253
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-78 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-121 36 - 87 CTLNC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-122 152 - 203 CTLNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-143 210 - 261 CPKNC 245 - 249 DPNHP 238 - 242 H-L Type (Type-I)
IRD-444 185 - 235 CTLNC 187 - 191 EASAE 204 - 208 L-H Type (Type-II)
IRD-540 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
IRD-551 69 - 119 CTLNC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C P R N C D T R I A Y S K C P R S


PICTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCTLNCDPRIFYSKCPRS
CTLNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C T L N C D P R I F Y S K C P R S


RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCTLNCDPRIFYSKCPRS
CTLNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C T L N C D P R I F Y S K C P R S


PICTNCCAGLKGCNYYNADGTFICEGESDPNHPKACPKNCDPNIAYSLCLYE
CPKNC
DPNHP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G L K G C N Y Y N A D G T F I C E G E S D P N H P K A C P K N C D P N I A Y S L C L Y E


KPCTLNCDPRIFYSKCPRSEASAEQPICTNCCAGLKGCNYYNADGTFICEG
CTLNC
EASAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T L N C D P R I F Y S K C P R S E A S A E Q P I C T N C C A G L K G C N Y Y N A D G T F I C E G


KPCPRNCDTRIAYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D T R I A Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


KPCTLNCDPRIFYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CTLNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T L N C D P R I F Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-78
 94 - 145 52
IRD-121
 36 - 87 52
IRD-122
 152 - 203 52
IRD-143
 210 - 261 52
IRD-444
 185 - 235 51
IRD-540
 127 - 177 51
IRD-551
 69 - 119 51
Amino Acid Count % by Frequency
Alanine 20 7.63
Cysteine 33 12.6
Aspartic acid 13 4.96
Glutamic acid 17 6.49
Phenylalanine 7 2.67
Glycine 19 7.25
Histidine 2 0.76
Isoleucine 13 4.96
Lysine 16 6.11
Leucine 12 4.58
Methionine 1 0.38
Asparagine 26 9.92
Proline 22 8.4
Glutamine 2 0.76
Arginine 13 4.96
Serine 18 6.87
Threonine 11 4.2
Valine 4 1.53
Tryptophan 0 0
Tyrosine 13 4.96