Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_280 [Q8W2K6]  : Proteinase inhibitor IIa

148Length

Solanum americanumSpecie

PIN2aGene Name

16324Mass

4Domain Count

Sequence :
     FASTA
MAVHKVSFLA CLLVLGWMFL LAKHVDAKAC TRECGHFSYG ICPRSEGSPQ KPICTNCCSG YKGCNYYSAK 70
GDLICEGESD PRNPKDCTFE CDTQIAYSKC PRSEGKMIIK PTGCTTCCTG YQGCYYFDQD GDFVCEGESP 140
EPKTTAYF 148
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVSFLACLLVLGWMFLLAKHVDA
 1 ↔ 27
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-263
52 - 103 52
IRD-408
85 - 137 53
IRD-457
28 - 77 50
IRD-460
28 - 76 49
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.596
0.4 -9.2 -15.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q8W2K6 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AF174381
REFERENCES
PubMed ID:     11785934    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-4 CTREC Trypsin 31 - 35
RL-4 CTREC Trypsin 31 - 35
RL-8 CTFEC Chymotrypsin 88 - 92
RL-8 CTFEC Chymotrypsin 88 - 92
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-263 52 - 103 CTFEC 87 - 91 DPRNP 80 - 84 H-L Type (Type-I)
IRD-408 85 - 137 CTFEC 87 - 91 - H+L Type (Type-III)
IRD-457 28 - 77 CTREC 30 - 34 EGSPQ 46 - 50 L-H Type (Type-II)
IRD-460 28 - 76 CTREC 30 - 34 EGSPQ 46 - 50 L-H Type (Type-II)
PICTNCCSGYKGCNYYSAKGDLICEGESDPRNPKDCTFECDTQIAYSKCPRS
CTFEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C S G Y K G C N Y Y S A K G D L I C E G E S D P R N P K D C T F E C D T Q I A Y S K C P R S


KDCTFECDTQIAYSKCPRSEGKMIIKPTGCTTCCTGYQGCYYFDQDGDFVCEG
CTFEC
H+L Type (Type-III)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
K D C T F E C D T Q I A Y S K C P R S E G K M I I K P T G C T T C C T G Y Q G C Y Y F D Q D G D F V C E G


KACTRECGHFSYGICPRSEGSPQKPICTNCCSGYKGCNYYSAKGDLICEG
CTREC
EGSPQ
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T R E C G H F S Y G I C P R S E G S P Q K P I C T N C C S G Y K G C N Y Y S A K G D L I C E G


KACTRECGHFSYGICPRSEGSPQKPICTNCCSGYKGCNYYSAKGDLICE
CTREC
EGSPQ
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
K A C T R E C G H F S Y G I C P R S E G S P Q K P I C T N C C S G Y K G C N Y Y S A K G D L I C E


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-263
 52 - 103 52
IRD-408
 85 - 137 53
IRD-457
 28 - 77 50
IRD-460
 28 - 76 49
Amino Acid Count % by Frequency
Alanine 8 5.41
Cysteine 17 11.49
Aspartic acid 8 5.41
Glutamic acid 9 6.08
Phenylalanine 7 4.73
Glycine 14 9.46
Histidine 3 2.03
Isoleucine 6 4.05
Lysine 11 7.43
Leucine 7 4.73
Methionine 3 2.03
Asparagine 3 2.03
Proline 9 6.08
Glutamine 4 2.7
Arginine 4 2.7
Serine 10 6.76
Threonine 10 6.76
Valine 5 3.38
Tryptophan 1 0.68
Tyrosine 9 6.08