Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_284 [B3F0C1]  : Proteinase inhibitor II

396Length

Nicotiana tabacumSpecie

-Gene Name

43274Mass

11Domain Count

Sequence :
     FASTA
MAVHKVSFLA LVLLFGMSLL VSNVEHADAK ACPFNCDPRI AYGVCPRLEE DKNNQICTNC CAGTKGCNYF 70
SADGTFICKG QSDPRNPKAC PRNCDPRIAY GVCPRLEEKK DNQICTNCCA GTKGCNYFSA DGTFVCKGQS 140
DPRNPKACPR NCDPRIAYGV CPRSEEKKDN QICTNCCAGT KGCNYFSADG TFFCKGQSDP RNPKACPPNC 210
DSRIDYGVCP RSEEKDNQIC TNCCAGTKGC NYFSADGTFV CKGQSDPRNP KACPRNCDPR IAYRVCPRSE 280
EKEDNQICTN CCAGTKGCNY FSANGTFVCK GQSDPRNPKA CPRNCDPRIA YGVCPRSEEE KNNQICTNCC 350
GGTKGCNYFS ANGTFICEGE SEYVSKVDEY VREVENDLQK SKVAVS 396
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVSFLALVLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-206
55 - 106 52
IRD-207
113 - 164 52
IRD-209
228 - 279 52
IRD-210
286 - 337 52
IRD-268
171 - 222 52
IRD-401
30 - 80 51
IRD-531
261 - 311 51
IRD-578
204 - 253 50
IRD-586
319 - 369 51
IRD-597
88 - 138 51
IRD-601
146 - 196 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.044
9.9 -13.4 -30.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
B3F0C1 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 EF408803
REFERENCES
PubMed ID:     19179349    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 322 - 326
RL-1 CPRNC Trypsin 322 - 326
RL-14 CPPNC Unknown 207 - 211
RL-14 CPPNC Unknown 207 - 211
RL-5 CPFNC Chymotrypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
ABA; hydrogen peroxide; methyl jasmonate Spodoptera litura; Helicoverpa armigera transgenic plants exhibited tolerance to sodium chloride, variable pH and sorbitol 19179349
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-206 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-207 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-209 228 - 279 CPRNC 263 - 267 DPRNP 256 - 260 H-L Type (Type-I)
IRD-210 286 - 337 CPRNC 321 - 325 DPRNP 314 - 318 H-L Type (Type-I)
IRD-268 171 - 222 CPPNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-401 30 - 80 CPFNC 32 - 36 EEDKN 49 - 53 L-H Type (Type-II)
IRD-531 261 - 311 CPRNC 263 - 267 EKEDN 281 - 285 L-H Type (Type-II)
IRD-578 204 - 253 CPPNC 206 - 210 EEKDN 223 - 227 L-H Type (Type-II)
IRD-586 319 - 369 CPRNC 321 - 325 EEKNN 339 - 343 L-H Type (Type-II)
IRD-597 88 - 138 CPRNC 90 - 94 EEKKD 107 - 111 L-H Type (Type-II)
IRD-601 146 - 196 CPRNC 148 - 152 EEKKD 165 - 169 L-H Type (Type-II)
QICTNCCAGTKGCNYFSADGTFICKGQSDPRNPKACPRNCDPRIAYGVCPRL
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F I C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R L


QICTNCCAGTKGCNYFSADGTFVCKGQSDPRNPKACPRNCDPRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGQSDPRNPKACPRNCDPRIAYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G Q S D P R N P K A C P R N C D P R I A Y R V C P R S


QICTNCCAGTKGCNYFSANGTFVCKGQSDPRNPKACPRNCDPRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F V C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R S


QICTNCCAGTKGCNYFSADGTFFCKGQSDPRNPKACPPNCDSRIDYGVCPRS
CPPNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F F C K G Q S D P R N P K A C P P N C D S R I D Y G V C P R S


KACPFNCDPRIAYGVCPRLEEDKNNQICTNCCAGTKGCNYFSADGTFICKG
CPFNC
EEDKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R L E E D K N N Q I C T N C C A G T K G C N Y F S A D G T F I C K G


KACPRNCDPRIAYRVCPRSEEKEDNQICTNCCAGTKGCNYFSANGTFVCKG
CPRNC
EKEDN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y R V C P R S E E K E D N Q I C T N C C A G T K G C N Y F S A N G T F V C K G


KACPPNCDSRIDYGVCPRSEEKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPPNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P P N C D S R I D Y G V C P R S E E K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGVCPRSEEEKNNQICTNCCGGTKGCNYFSANGTFICEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E E K N N Q I C T N C C G G T K G C N Y F S A N G T F I C E G


KACPRNCDPRIAYGVCPRLEEKKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R L E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGVCPRSEEKKDNQICTNCCAGTKGCNYFSADGTFFCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F F C K G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-206
 55 - 106 52
IRD-207
 113 - 164 52
IRD-209
 228 - 279 52
IRD-210
 286 - 337 52
IRD-268
 171 - 222 52
IRD-401
 30 - 80 51
IRD-531
 261 - 311 51
IRD-578
 204 - 253 50
IRD-586
 319 - 369 51
IRD-597
 88 - 138 51
IRD-601
 146 - 196 51
Amino Acid Count % by Frequency
Alanine 27 6.82
Cysteine 48 12.12
Aspartic acid 24 6.06
Glutamic acid 21 5.3
Phenylalanine 16 4.04
Glycine 31 7.83
Histidine 2 0.51
Isoleucine 14 3.54
Lysine 29 7.32
Leucine 9 2.27
Methionine 2 0.51
Asparagine 35 8.84
Proline 28 7.07
Glutamine 12 3.03
Arginine 23 5.81
Serine 23 5.81
Threonine 18 4.55
Valine 20 5.05
Tryptophan 0 0
Tyrosine 14 3.54