Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_285 [E9JGT1]  : Serine proteinase inhibitor 2c-R3 (Fragment)

174Length

Solanum nigrumSpecie

SPI2c-R3Gene Name

19125Mass

4Domain Count

Sequence :
     FASTA
CDTRIDYGIC PVSETKRINQ ICTNCCAGKK GCMYFSDDGT FICEGESEYV SEVPVDNKPC PRNCDGRIAY 70
GICPLSETKR INQICTNCCA GKKGCMYFSN DGTFICEGES EYVSEVPVDN KPCPRNCDGR IAYGICPLSE 140
NKRINQICTN CCAGKKGCLY FSNDGTFICE GESE 174
SIGNAL PEPTIDE
SEQUENCE Position
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-2
20 - 76 57
IRD-3
83 - 139 57
IRD-515
58 - 108 51
IRD-516
121 - 171 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.468
-3.9 -14.7 -22.9
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
E9JGT1 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 GU133373
REFERENCES
PubMed ID:     21177479    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 61 - 65
RL-1 CPRNC Trypsin 61 - 65
RL-1 CPRNC Trypsin 124 - 128
RL-1 CPRNC Trypsin 124 - 128
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves,Flowers Wounding, Oral secretion Oral secretion,Methyl Jasmonate Manduca sexta 21177479
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-2 20 - 76 CPRNC 60 - 64 - H+L Type (Type-III)
IRD-3 83 - 139 CPRNC 123 - 127 - H+L Type (Type-III)
IRD-515 58 - 108 CPRNC 60 - 64 ETKRI 77 - 81 L-H Type (Type-II)
IRD-516 121 - 171 CPRNC 123 - 127 - H+L Type (Type-III)
QICTNCCAGKKGCMYFSDDGTFICEGESEYVSEVPVDNKPCPRNCDGRIAYGICPLS
CPRNC
H+L Type (Type-III)
3,45 ; 6,24 ; 7,41 ; 13,54
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57
Q I C T N C C A G K K G C M Y F S D D G T F I C E G E S E Y V S E V P V D N K P C P R N C D G R I A Y G I C P L S


QICTNCCAGKKGCMYFSNDGTFICEGESEYVSEVPVDNKPCPRNCDGRIAYGICPLS
CPRNC
H+L Type (Type-III)
3,45 ; 6,24 ; 7,41 ; 13,54
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57
Q I C T N C C A G K K G C M Y F S N D G T F I C E G E S E Y V S E V P V D N K P C P R N C D G R I A Y G I C P L S


KPCPRNCDGRIAYGICPLSETKRINQICTNCCAGKKGCMYFSNDGTFICEG
CPRNC
ETKRI
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E T K R I N Q I C T N C C A G K K G C M Y F S N D G T F I C E G


KPCPRNCDGRIAYGICPLSENKRINQICTNCCAGKKGCLYFSNDGTFICEG
CPRNC
H+L Type (Type-III)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E N K R I N Q I C T N C C A G K K G C L Y F S N D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-2
 20 - 76 57
IRD-3
 83 - 139 57
IRD-515
 58 - 108 51
IRD-516
 121 - 171 51
Amino Acid Count % by Frequency
Alanine 5 2.87
Cysteine 23 13.22
Aspartic acid 10 5.75
Glutamic acid 14 8.05
Phenylalanine 6 3.45
Glycine 17 9.77
Histidine 0 0
Isoleucine 15 8.62
Lysine 11 6.32
Leucine 3 1.72
Methionine 2 1.15
Asparagine 13 7.47
Proline 9 5.17
Glutamine 3 1.72
Arginine 8 4.6
Serine 11 6.32
Threonine 9 5.17
Valine 7 4.02
Tryptophan 0 0
Tyrosine 8 4.6