Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_286 [Q1WL49]  : Trypsin proteinase inhibitor

395Length

Nicotiana occidentalis subsp. hesperisSpecie

PIGene Name

43470Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLFFGMSLL VSNVEHAKAC TRECDTRIAY GVCPRLEEKT NNRICTNCCA GKKGCKYFSD 70
DGTFVCEGES DPRNPKPCPR NCDGRIAYGI CPLSEEKKNN RLCTNCCAGM KGCNYFSADG TFVCEGESDP 140
RNPKPCPRNC DGRIAYGICP LSEEKKNNRL CTNCCAGTKG CNYFSADGTF VCEGESDPRN PKPCPRNCDG 210
RIAYGICPLS EEKKNNRLCT NCCAGTKGCN YFGADGTFVC EGESDPRNPK RCTRECDPRI NYRICPRSEE 280
KKNNRICTNC CSGKKGCKYF SDDGTFVCEG ESDPRNPKGC TIECDPRIAY GICPLSEEKK NNRLCTNCCA 350
GKKGCNYFSA NGTFICEGES EYASKVVEYV GEVEDDLQKS KVAVS 395
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLFFGMSLLVSNVEHAKA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-211
53 - 104 52
IRD-212
111 - 162 52
IRD-213
169 - 220 52
IRD-257
227 - 278 52
IRD-269
285 - 336 52
IRD-449
28 - 78 51
IRD-639
86 - 136 51
IRD-640
144 - 194 51
IRD-641
202 - 252 51
IRD-662
260 - 310 51
IRD-672
318 - 368 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.006
9.6 -14.4 -31.9
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL49 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158183
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 89 - 93
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 147 - 151
RL-1 CPRNC Trypsin 205 - 209
RL-1 CPRNC Trypsin 205 - 209
RL-15 CTIEC Chymotrypsin 321 - 325
RL-15 CTIEC Chymotrypsin 321 - 325
RL-4 CTREC Trypsin 31 - 35
RL-4 CTREC Trypsin 263 - 267
RL-4 CTREC Trypsin 263 - 267
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-211 53 - 104 CPRNC 88 - 92 DPRNP 81 - 85 H-L Type (Type-I)
IRD-212 111 - 162 CPRNC 146 - 150 DPRNP 139 - 143 H-L Type (Type-I)
IRD-213 169 - 220 CPRNC 204 - 208 DPRNP 197 - 201 H-L Type (Type-I)
IRD-257 227 - 278 CTREC 262 - 266 DPRNP 255 - 259 H-L Type (Type-I)
IRD-269 285 - 336 CTIEC 320 - 324 DPRNP 313 - 317 H-L Type (Type-I)
IRD-449 28 - 78 CTREC 30 - 34 EEKTN 47 - 51 L-H Type (Type-II)
IRD-639 86 - 136 CPRNC 88 - 92 EEKKN 105 - 109 L-H Type (Type-II)
IRD-640 144 - 194 CPRNC 146 - 150 EEKKN 163 - 167 L-H Type (Type-II)
IRD-641 202 - 252 CPRNC 204 - 208 EEKKN 221 - 225 L-H Type (Type-II)
IRD-662 260 - 310 CTREC 262 - 266 EEKKN 279 - 283 L-H Type (Type-II)
IRD-672 318 - 368 CTIEC 320 - 324 EEKKN 337 - 341 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGMKGCNYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G M K G C N Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGTKGCNYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P L S


RLCTNCCAGTKGCNYFGADGTFVCEGESDPRNPKRCTRECDPRINYRICPRS
CTREC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G T K G C N Y F G A D G T F V C E G E S D P R N P K R C T R E C D P R I N Y R I C P R S


RICTNCCSGKKGCKYFSDDGTFVCEGESDPRNPKGCTIECDPRIAYGICPLS
CTIEC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C S G K K G C K Y F S D D G T F V C E G E S D P R N P K G C T I E C D P R I A Y G I C P L S


KACTRECDTRIAYGVCPRLEEKTNNRICTNCCAGKKGCKYFSDDGTFVCEG
CTREC
EEKTN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R E C D T R I A Y G V C P R L E E K T N N R I C T N C C A G K K G C K Y F S D D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G M K G C N Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G T K G C N Y F S A D G T F V C E G


KPCPRNCDGRIAYGICPLSEEKKNNRLCTNCCAGTKGCNYFGADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E E K K N N R L C T N C C A G T K G C N Y F G A D G T F V C E G


KRCTRECDPRINYRICPRSEEKKNNRICTNCCSGKKGCKYFSDDGTFVCEG
CTREC
EEKKN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K R C T R E C D P R I N Y R I C P R S E E K K N N R I C T N C C S G K K G C K Y F S D D G T F V C E G


KGCTIECDPRIAYGICPLSEEKKNNRLCTNCCAGKKGCNYFSANGTFICEG
CTIEC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C T I E C D P R I A Y G I C P L S E E K K N N R L C T N C C A G K K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-211
 53 - 104 52
IRD-212
 111 - 162 52
IRD-213
 169 - 220 52
IRD-257
 227 - 278 52
IRD-269
 285 - 336 52
IRD-449
 28 - 78 51
IRD-639
 86 - 136 51
IRD-640
 144 - 194 51
IRD-641
 202 - 252 51
IRD-662
 260 - 310 51
IRD-672
 318 - 368 51
Amino Acid Count % by Frequency
Alanine 20 5.06
Cysteine 48 12.15
Aspartic acid 20 5.06
Glutamic acid 32 8.1
Phenylalanine 15 3.8
Glycine 36 9.11
Histidine 2 0.51
Isoleucine 15 3.8
Lysine 31 7.85
Leucine 16 4.05
Methionine 3 0.76
Asparagine 33 8.35
Proline 24 6.08
Glutamine 1 0.25
Arginine 27 6.84
Serine 23 5.82
Threonine 19 4.81
Valine 16 4.05
Tryptophan 0 0
Tyrosine 14 3.54