Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_288 [Q3SBE6]  : Insect injury-induced proteinase inhibitor

281Length

Nicotiana tabacumSpecie

-Gene Name

30824Mass

7Domain Count

Sequence :
     FASTA
MAVHKVSFLA LLLLLGMSLL VSNVEHADAK ACPLNCDPRI AYGVCPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFVCEG ESDPRNPKAC PRNCDPRIAY GICPLSEEKK NDRICTNCCA GKKGCKYFSD DGTFVCEGES 140
DPRNPKACTL NCDPRIAYGI CPLSEEKKND RICTNCCAGK KGCKYFSDDG TFVCEGESDP RNPKACPRNC 210
DGRIAYGICP LSEEKKNDRI CTNCCAGKKG CKYFSDDGTF ICEGESEYAS KVDEYVREVE NDLQKSKVAV 280
S 281
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVSFLALLLLLGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-152
55 - 106 52
IRD-153
171 - 222 52
IRD-255
113 - 164 52
IRD-606
204 - 254 51
IRD-653
88 - 138 51
IRD-655
30 - 80 51
IRD-658
146 - 196 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.949
2 -14.7 -26.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q3SBE6 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ071272
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-2 CPLNC Chymotrypsin 33 - 37
RL-3 CTLNC Chymotrypsin 149 - 153
RL-3 CTLNC Chymotrypsin 149 - 153
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-152 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-153 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-255 113 - 164 CTLNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-606 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-653 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-655 30 - 80 CPLNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-658 146 - 196 CTLNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G I C P L S


RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACTLNCDPRIAYGICPLS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C T L N C D P R I A Y G I C P L S


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F I C E G


KACPRNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACPLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-152
 55 - 106 52
IRD-153
 171 - 222 52
IRD-255
 113 - 164 52
IRD-606
 204 - 254 51
IRD-653
 88 - 138 51
IRD-655
 30 - 80 51
IRD-658
 146 - 196 51
Amino Acid Count % by Frequency
Alanine 18 6.41
Cysteine 32 11.39
Aspartic acid 22 7.83
Glutamic acid 21 7.47
Phenylalanine 9 3.2
Glycine 22 7.83
Histidine 2 0.71
Isoleucine 12 4.27
Lysine 27 9.61
Leucine 14 4.98
Methionine 2 0.71
Asparagine 17 6.05
Proline 16 5.69
Glutamine 1 0.36
Arginine 15 5.34
Serine 18 6.41
Threonine 10 3.56
Valine 13 4.63
Tryptophan 0 0
Tyrosine 10 3.56