Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_289 [A0A1S4BIG0]  : proteinase inhibitor type-2 P303.51-like

168Length

Nicotiana tabacumSpecie

LOC107808639Gene Name

18564Mass

3Domain Count

Sequence :
     FASTA
MAVHKAGFLS LLLLFGIFLL LSELEYADAK ACTRNCNPRI RYGRCPKSGN KKFSLGCTNC CAGSLGCNYY 70
SANGTFICEG ESEIKVETIE TEVETMVEPN KICPRNCDPK IRYGRCPKSG NKKFNVGCTN CCAARKGCNY 140
FSANGTFVCE GETETKVEII ETKVEMRK 168
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKAGFLSLLLLFGIFLLLSELEYADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-5
55 - 114 60
IRD-523
101 - 151 51
IRD-530
30 - 80 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.672
7.7 -0.9 -7.1
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1S4BIG0 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 104 - 108
RL-1 CPRNC Trypsin 104 - 108
RL-30 CTRNC Trypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-5 55 - 114 CPRNC 103 - 107 - H+L Type (Type-III)
IRD-523 101 - 151 CPRNC 103 - 107 GNKKF 120 - 124 L-H Type (Type-II)
IRD-530 30 - 80 CTRNC 32 - 36 GNKKF 49 - 53 L-H Type (Type-II)
LGCTNCCAGSLGCNYYSANGTFICEGESEIKVETIETEVETMVEPNKICPRNCDPKIRYG
CPRNC
H+L Type (Type-III)
7,49 ; 24,53
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
L G C T N C C A G S L G C N Y Y S A N G T F I C E G E S E I K V E T I E T E V E T M V E P N K I C P R N C D P K I R Y G


KICPRNCDPKIRYGRCPKSGNKKFNVGCTNCCAARKGCNYFSANGTFVCEG
CPRNC
GNKKF
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K I C P R N C D P K I R Y G R C P K S G N K K F N V G C T N C C A A R K G C N Y F S A N G T F V C E G


KACTRNCNPRIRYGRCPKSGNKKFSLGCTNCCAGSLGCNYYSANGTFICEG
CTRNC
GNKKF
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R N C N P R I R Y G R C P K S G N K K F S L G C T N C C A G S L G C N Y Y S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-5
 55 - 114 60
IRD-523
 101 - 151 51
IRD-530
 30 - 80 51
Amino Acid Count % by Frequency
Alanine 10 5.95
Cysteine 16 9.52
Aspartic acid 2 1.19
Glutamic acid 16 9.52
Phenylalanine 8 4.76
Glycine 15 8.93
Histidine 1 0.6
Isoleucine 9 5.36
Lysine 15 8.93
Leucine 11 6.55
Methionine 3 1.79
Asparagine 13 7.74
Proline 6 3.57
Glutamine 0 0
Arginine 9 5.36
Serine 9 5.36
Threonine 11 6.55
Valine 8 4.76
Tryptophan 0 0
Tyrosine 6 3.57