Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_290 [Q40378]  : Proteinase inhibitor

397Length

Nicotiana alataSpecie

-Gene Name

43415Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSNVEHADAK ACTLNCDPRI AYGVCPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFVCEG ESDPRNPKAC TLNCDPRIAY GVCPRSEEKK NDRICTNCCA GTKGCKYFSD DGTFVCEGES 140
DPRNPKACPR NCDPRIAYGI CPLAEEKKND RICTNCCAGK KGCKYFSDDG TFVCEGESDP KNPKACPRNC 210
DGRIAYGICP LSEEKKNDRI CTNCCAGKKG CKYFSDDGTF VCEGESDPKN PKACPRNCDG RIAYGICPLS 280
EEKKNDRICT NCCAGKKGCK YFSDDGTFVC EGESDPRNPK ACPRNCDGRI AYGICPLSEE KKNDRICTNC 350
CAGKKGCKYF SDDGTFICEG ESEYASKVDE YVGEVENDLQ KSKVAVS 397
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-153
287 - 338 52
IRD-191
113 - 164 52
IRD-192
171 - 222 52
IRD-192
229 - 280 52
IRD-253
55 - 106 52
IRD-606
320 - 370 51
IRD-623
146 - 196 51
IRD-624
204 - 254 51
IRD-624
262 - 312 51
IRD-661
30 - 80 51
IRD-661
88 - 138 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.205
3.7 -20.6 -38.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q40378 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 U08219
REFERENCES
PubMed ID:     8453302    | 7578034    | 8555206    | 10360353    | 10425681    | 11178894    | 18991765    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-3 CTLNC Chymotrypsin 33 - 37
RL-3 CTLNC Chymotrypsin 91 - 95
RL-3 CTLNC Chymotrypsin 91 - 95
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flower 8453302
Flower 7578034
Synthesis and structure determination by NMR of a 26-residue peptide encompassing putative vacuolar targeting sequence 8555206
Bracelet structure of PI elucidated by NMR Spectroscopy 10360353
First report showing that a single repeat is capable of folding into a compact globular domain that displays native-like PI activity 10425681
The solution structure of C1-T1, a two-domain PI showing clasped bracelet structure. 11178894
Structural refinement of NMR structures 18991765
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
Chymotrypsin, Trypsin 8453302
Chymotrypsin, Trypsin 7578034
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
1 4 7578034
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-153 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-191 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-192 171 - 222 CPRNC 206 - 210 DPKNP 199 - 203 H-L Type (Type-I)
IRD-192 229 - 280 CPRNC 264 - 268 DPKNP 257 - 261 H-L Type (Type-I)
IRD-253 55 - 106 CTLNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-606 320 - 370 CPRNC 322 - 326 EEKKN 339 - 343 L-H Type (Type-II)
IRD-623 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-624 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-624 262 - 312 CPRNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
IRD-661 30 - 80 CTLNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-661 88 - 138 CTLNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYGICPLA
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G I C P L A


RICTNCCAGKKGCKYFSDDGTFVCEGESDPKNPKACPRNCDGRIAYGICPLS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P K N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGKKGCKYFSDDGTFVCEGESDPKNPKACPRNCDGRIAYGICPLS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P K N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACTLNCDPRIAYGVCPRS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C T L N C D P R I A Y G V C P R S


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F I C E G


KACPRNCDPRIAYGICPLAEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L A E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-153
 287 - 338 52
IRD-191
 113 - 164 52
IRD-192
 171 - 222 52
IRD-192
 229 - 280 52
IRD-253
 55 - 106 52
IRD-606
 320 - 370 51
IRD-623
 146 - 196 51
IRD-624
 204 - 254 51
IRD-624
 262 - 312 51
IRD-661
 30 - 80 51
IRD-661
 88 - 138 51
Amino Acid Count % by Frequency
Alanine 25 6.3
Cysteine 48 12.09
Aspartic acid 32 8.06
Glutamic acid 29 7.3
Phenylalanine 14 3.53
Glycine 35 8.82
Histidine 2 0.5
Isoleucine 17 4.28
Lysine 39 9.82
Leucine 14 3.53
Methionine 2 0.5
Asparagine 25 6.3
Proline 23 5.79
Glutamine 1 0.25
Arginine 22 5.54
Serine 23 5.79
Threonine 16 4.03
Valine 16 4.03
Tryptophan 0 0
Tyrosine 14 3.53