Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_292 [A0A1S4CNR9]  : wound-induced proteinase inhibitor 2-like isoform X3

389Length

Nicotiana tabacumSpecie

LOC107821006Gene Name

42475Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLFGMSLLV SNVEYADAKA CPRNCDGRIA YGICPRSEEK KDNQICTNCC AGTKGCNYFS 70
ADGTFVCEGE SDPRNPKACP RNCDGRIAYG VCPRSEEKKD NQICTNCCAG TKGCNYFSAD GTFVCEGESD 140
PRNPKACPRN CDGRIAYGVC PRSEEKKDNQ ICTNCCAGTK GCNYFSADGT FVCEGESDPR NSKACPRNCD 210
GRIAYGVCRS SEEKKNNQIC TNCCAGTKGC NYFSADGTFV CKGKFDPRNP RPCPRNCDPR ITYGVCPRSE 280
EKKNNQICTN CCAGIKGCNY FSVDGTFVCE GESDPRNSKA CPRNCDPRIT YGVCPRSEEK KNNQICTNCC 350
AGMKGCNYFS VNGTFICEGE SEYANKVEND LQKSKVAVF 389
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLFGMSLLVSNVEYADA
 1 ↔ 28
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-74
170 - 221 52
IRD-75
286 - 337 52
IRD-172
54 - 105 52
IRD-172
112 - 163 52
IRD-184
228 - 279 52
IRD-595
29 - 79 51
IRD-596
145 - 195 51
IRD-596
87 - 137 51
IRD-625
203 - 253 51
IRD-626
261 - 311 51
IRD-628
319 - 369 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.041
9.5 -13.3 -30.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1S4CNR9 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 32 - 36
RL-1 CPRNC Trypsin 90 - 94
RL-1 CPRNC Trypsin 90 - 94
RL-1 CPRNC Trypsin 148 - 152
RL-1 CPRNC Trypsin 148 - 152
RL-1 CPRNC Trypsin 206 - 210
RL-1 CPRNC Trypsin 206 - 210
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 322 - 326
RL-1 CPRNC Trypsin 322 - 326
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-74 170 - 221 CPRNC 205 - 209 DPRNS 198 - 202 H-L Type (Type-I)
IRD-75 286 - 337 CPRNC 321 - 325 DPRNS 314 - 318 H-L Type (Type-I)
IRD-172 54 - 105 CPRNC 89 - 93 DPRNP 82 - 86 H-L Type (Type-I)
IRD-172 112 - 163 CPRNC 147 - 151 DPRNP 140 - 144 H-L Type (Type-I)
IRD-184 228 - 279 CPRNC 263 - 267 DPRNP 256 - 260 H-L Type (Type-I)
IRD-595 29 - 79 CPRNC 31 - 35 EEKKD 48 - 52 L-H Type (Type-II)
IRD-596 145 - 195 CPRNC 147 - 151 EEKKD 164 - 168 L-H Type (Type-II)
IRD-596 87 - 137 CPRNC 89 - 93 EEKKD 106 - 110 L-H Type (Type-II)
IRD-625 203 - 253 CPRNC 205 - 209 EEKKN 222 - 226 L-H Type (Type-II)
IRD-626 261 - 311 CPRNC 263 - 267 EEKKN 280 - 284 L-H Type (Type-II)
IRD-628 319 - 369 CPRNC 321 - 325 EEKKN 338 - 342 L-H Type (Type-II)
QICTNCCAGTKGCNYFSADGTFVCEGESDPRNSKACPRNCDGRIAYGVCRSS
CPRNC
DPRNS
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N S K A C P R N C D G R I A Y G V C R S S


QICTNCCAGIKGCNYFSVDGTFVCEGESDPRNSKACPRNCDPRITYGVCPRS
CPRNC
DPRNS
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G I K G C N Y F S V D G T F V C E G E S D P R N S K A C P R N C D P R I T Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDGRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCEGESDPRNPKACPRNCDGRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGKFDPRNPRPCPRNCDPRITYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G K F D P R N P R P C P R N C D P R I T Y G V C P R S


KACPRNCDGRIAYGICPRSEEKKDNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRSEEKKDNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCPRSEEKKDNQICTNCCAGTKGCNYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C P R S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGVCRSSEEKKNNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,32 ; 7,16 ; 28,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G V C R S S E E K K N N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


RPCPRNCDPRITYGVCPRSEEKKNNQICTNCCAGIKGCNYFSVDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
R P C P R N C D P R I T Y G V C P R S E E K K N N Q I C T N C C A G I K G C N Y F S V D G T F V C E G


KACPRNCDPRITYGVCPRSEEKKNNQICTNCCAGMKGCNYFSVNGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I T Y G V C P R S E E K K N N Q I C T N C C A G M K G C N Y F S V N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-74
 170 - 221 52
IRD-75
 286 - 337 52
IRD-172
 54 - 105 52
IRD-172
 112 - 163 52
IRD-184
 228 - 279 52
IRD-595
 29 - 79 51
IRD-596
 145 - 195 51
IRD-596
 87 - 137 51
IRD-625
 203 - 253 51
IRD-626
 261 - 311 51
IRD-628
 319 - 369 51
Amino Acid Count % by Frequency
Alanine 25 6.43
Cysteine 48 12.34
Aspartic acid 21 5.4
Glutamic acid 25 6.43
Phenylalanine 16 4.11
Glycine 35 9
Histidine 1 0.26
Isoleucine 15 3.86
Lysine 28 7.2
Leucine 7 1.8
Methionine 3 0.77
Asparagine 36 9.25
Proline 22 5.66
Glutamine 7 1.8
Arginine 25 6.43
Serine 24 6.17
Threonine 18 4.63
Valine 19 4.88
Tryptophan 0 0
Tyrosine 14 3.6