Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_295 [A0A1J6JJ91]  : Proteinase inhibitor type-2

197Length

Nicotiana attenuataSpecie

IP21 A4A49_08755Gene Name

21088Mass

5Domain Count

Sequence :
     FASTA
MAVHKVSFLA HLLVLGMFLL LVDAKACTKE CGNFAYGICP RSQGTPDHPI CTTCCAGYKG CNYYNANGTF 70
ICEGLSDPKN PNFCPQFCDP DIAYSKCPRS EGETIINPTG CTTCCTGYKG CYYFGQDGEF VCEGESDEPK 140
SCTTECDPRV ATISCPISGL VKINQECINC CNADKGCELY DNDGSLICTG GEPQSAA 197
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVSFLAHLLVLGMFLLLVDA
 1 ↔ 24
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-42
109 - 158 50
IRD-267
49 - 100 52
IRD-293
140 - 190 51
IRD-420
25 - 74 50
IRD-428
82 - 134 53
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.209
-7.3 -20.1 -28.5
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1J6JJ91 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 MJEQ01037184
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-10 CTKEC Trypsin 28 - 32
RL-12 CPQFC Unknown 85 - 89
RL-12 CPQFC Unknown 85 - 89
RL-24 CTTEC Unknown 143 - 147
RL-24 CTTEC Unknown 143 - 147
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-42 109 - 158 CTTEC 142 - 146 ESDEP 135 - 139 H-L Type (Type-I)
IRD-267 49 - 100 CPQFC 84 - 88 DPKNP 77 - 81 H-L Type (Type-I)
IRD-293 140 - 190 CTTEC 142 - 146 GLVKI 159 - 163 L-H Type (Type-II)
IRD-420 25 - 74 CTKEC 27 - 31 - H+L Type (Type-III)
IRD-428 82 - 134 CPQFC 84 - 88 EGETI 101 - 105 L-H Type (Type-II)
TGCTTCCTGYKGCYYFGQDGEFVCEGESDEPKSCTTECDPRVATISCPIS
CTTEC
ESDEP
H-L Type (Type-I)
3,38 ; 6,24 ; 7,34 ; 13,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G E S D E P K S C T T E C D P R V A T I S C P I S


PICTTCCAGYKGCNYYNANGTFICEGLSDPKNPNFCPQFCDPDIAYSKCPRS
CPQFC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T T C C A G Y K G C N Y Y N A N G T F I C E G L S D P K N P N F C P Q F C D P D I A Y S K C P R S


KSCTTECDPRVATISCPISGLVKINQECINCCNADKGCELYDNDGSLICTG
CTTEC
GLVKI
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K S C T T E C D P R V A T I S C P I S G L V K I N Q E C I N C C N A D K G C E L Y D N D G S L I C T G


KACTKECGNFAYGICPRSQGTPDHPICTTCCAGYKGCNYYNANGTFICEG
CTKEC
H+L Type (Type-III)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C T K E C G N F A Y G I C P R S Q G T P D H P I C T T C C A G Y K G C N Y Y N A N G T F I C E G


NFCPQFCDPDIAYSKCPRSEGETIINPTGCTTCCTGYKGCYYFGQDGEFVCEG
CPQFC
EGETI
L-H Type (Type-II)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
N F C P Q F C D P D I A Y S K C P R S E G E T I I N P T G C T T C C T G Y K G C Y Y F G Q D G E F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-42
 109 - 158 50
IRD-267
 49 - 100 52
IRD-293
 140 - 190 51
IRD-420
 25 - 74 50
IRD-428
 82 - 134 53
Amino Acid Count % by Frequency
Alanine 12 6.09
Cysteine 24 12.18
Aspartic acid 11 5.58
Glutamic acid 12 6.09
Phenylalanine 8 4.06
Glycine 20 10.15
Histidine 3 1.52
Isoleucine 11 5.58
Lysine 10 5.08
Leucine 11 5.58
Methionine 2 1.02
Asparagine 11 5.58
Proline 13 6.6
Glutamine 5 2.54
Arginine 3 1.52
Serine 11 5.58
Threonine 14 7.11
Valine 7 3.55
Tryptophan 0 0
Tyrosine 9 4.57