Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_297 [Q6H238]  : Putative 6 repeat proteinase inhibitor

410Length

Nicotiana attenuataSpecie

-Gene Name

46496Mass

10Domain Count

Sequence :
     FASTA
MWNMQMPRLV PLTVIQELPR EFARVQKKRK TIKYAPTVAQ ARRDVTTLVL MELLFVNENL ILEIPKLVPE 70
IVIQEIDYRV CPRSEEKNNQ ICTNCCAGTM GCNYFSADGS FVCKGESDLR NPKDCPRNCD PRIDYRVCRR 140
SEEKNNQICT NCCVGTMGCN YFSADGSFVC KGESDLRNPK DCPRNCDPRI DYRVCRRSEE KNNQICTNCC 210
AGTKGYNYFS ADGTFVCKGE SDPRNPKACP RNCDPRIDYR FCPRSEEKDN QICTNCCAGT KGCNYFSADG 280
TFVCKGESDP RNPKACPRNC DPRIDYRVCP RSEENNNQKC TNCCAGTKGC NYFSANGTFI CEGEYDPRNP 350
KACPRNCDPR IDYRVCPRSE ENNNQKCTNC CAGTKGCNYF SANGTFICEG ESEYVRKVDE 410
SIGNAL PEPTIDE
SEQUENCE Position
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-79
90 - 141 52
IRD-80
147 - 198 52
IRD-176
261 - 312 52
IRD-219
204 - 255 52
IRD-220
318 - 369 52
IRD-508
294 - 343 50
IRD-508
351 - 400 50
IRD-576
237 - 286 50
IRD-587
123 - 172 50
IRD-588
180 - 229 50
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.167
9.4 -12.1 -28.2
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q6H238 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 AY426751
REFERENCES
PubMed ID:     15133153    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 126 - 130
RL-1 CPRNC Trypsin 126 - 130
RL-1 CPRNC Trypsin 183 - 187
RL-1 CPRNC Trypsin 183 - 187
RL-1 CPRNC Trypsin 240 - 244
RL-1 CPRNC Trypsin 240 - 244
RL-1 CPRNC Trypsin 297 - 301
RL-1 CPRNC Trypsin 297 - 301
RL-1 CPRNC Trypsin 354 - 358
RL-1 CPRNC Trypsin 354 - 358
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
insect attack jasmonate; salicylate Manduca sexta 15133153
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-79 90 - 141 CPRNC 125 - 129 DLRNP 118 - 122 H-L Type (Type-I)
IRD-80 147 - 198 CPRNC 182 - 186 DLRNP 175 - 179 H-L Type (Type-I)
IRD-176 261 - 312 CPRNC 296 - 300 DPRNP 289 - 293 H-L Type (Type-I)
IRD-219 204 - 255 CPRNC 239 - 243 DPRNP 232 - 236 H-L Type (Type-I)
IRD-220 318 - 369 CPRNC 353 - 357 DPRNP 346 - 350 H-L Type (Type-I)
IRD-508 294 - 343 CPRNC 296 - 300 - H+L Type (Type-III)
IRD-508 351 - 400 CPRNC 353 - 357 - H+L Type (Type-III)
IRD-576 237 - 286 CPRNC 239 - 243 EEKDN 256 - 260 L-H Type (Type-II)
IRD-587 123 - 172 CPRNC 125 - 129 EEKNN 142 - 146 L-H Type (Type-II)
IRD-588 180 - 229 CPRNC 182 - 186 EEKNN 199 - 203 L-H Type (Type-II)
QICTNCCAGTMGCNYFSADGSFVCKGESDLRNPKDCPRNCDPRIDYRVCRRS
CPRNC
DLRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T M G C N Y F S A D G S F V C K G E S D L R N P K D C P R N C D P R I D Y R V C R R S


QICTNCCVGTMGCNYFSADGSFVCKGESDLRNPKDCPRNCDPRIDYRVCRRS
CPRNC
DLRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C V G T M G C N Y F S A D G S F V C K G E S D L R N P K D C P R N C D P R I D Y R V C R R S


QICTNCCAGTKGCNYFSADGTFVCKGESDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R V C P R S


QICTNCCAGTKGYNYFSADGTFVCKGESDPRNPKACPRNCDPRIDYRFCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,36 ; 7,40 ; 24,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G Y N Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D Y R F C P R S


QKCTNCCAGTKGCNYFSANGTFICEGEYDPRNPKACPRNCDPRIDYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q K C T N C C A G T K G C N Y F S A N G T F I C E G E Y D P R N P K A C P R N C D P R I D Y R V C P R S


KACPRNCDPRIDYRVCPRSEENNNQKCTNCCAGTKGCNYFSANGTFICEG
CPRNC
H+L Type (Type-III)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E N N N Q K C T N C C A G T K G C N Y F S A N G T F I C E G


KACPRNCDPRIDYRVCPRSEENNNQKCTNCCAGTKGCNYFSANGTFICEG
CPRNC
H+L Type (Type-III)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R V C P R S E E N N N Q K C T N C C A G T K G C N Y F S A N G T F I C E G


KACPRNCDPRIDYRFCPRSEEKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y R F C P R S E E K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KDCPRNCDPRIDYRVCRRSEEKNNQICTNCCVGTMGCNYFSADGSFVCKG
CPRNC
EEKNN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K D C P R N C D P R I D Y R V C R R S E E K N N Q I C T N C C V G T M G C N Y F S A D G S F V C K G


KDCPRNCDPRIDYRVCRRSEEKNNQICTNCCAGTKGYNYFSADGTFVCKG
CPRNC
EEKNN
L-H Type (Type-II)
3,31 ; 7,30 ; 16,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K D C P R N C D P R I D Y R V C R R S E E K N N Q I C T N C C A G T K G Y N Y F S A D G T F V C K G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-79
 90 - 141 52
IRD-80
 147 - 198 52
IRD-176
 261 - 312 52
IRD-219
 204 - 255 52
IRD-220
 318 - 369 52
IRD-508
 294 - 343 50
IRD-508
 351 - 400 50
IRD-576
 237 - 286 50
IRD-587
 123 - 172 50
IRD-588
 180 - 229 50
Amino Acid Count % by Frequency
Alanine 18 4.39
Cysteine 45 10.98
Aspartic acid 25 6.1
Glutamic acid 29 7.07
Phenylalanine 15 3.66
Glycine 24 5.85
Histidine 0 0
Isoleucine 18 4.39
Lysine 25 6.1
Leucine 12 2.93
Methionine 6 1.46
Asparagine 40 9.76
Proline 28 6.83
Glutamine 11 2.68
Arginine 36 8.78
Serine 19 4.63
Threonine 21 5.12
Valine 21 5.12
Tryptophan 1 0.24
Tyrosine 16 3.9