Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_3 [P01080]  : Proteinase inhibitor type-2 K (Proteinase inhibitor type II K) (IIK) [Cleaved into: Proteinase inhibitor PCI-1 (Proteinase inhibitor PCI-I)]

153Length

Solanum tuberosumSpecie

PIN2KGene Name

16505Mass

3Domain Count

Sequence :
     FASTA
MDVHKEVNFV AYLLIVLGLL VLVSAMDVDA KACIRECGNL GFGICPRSEG SPENPICTNC CAGYKGCNYY 70
SANGAFICEG QSDPKKPKAC PLNCDPHIAY SKCPRSEGKS LIYPTGCTTC CTGYKGCYYF GKNGKFVCEG 140
ESDEPKANMY PAM 153
SIGNAL PEPTIDE
SEQUENCE Position
MDVHKEVNFVAYLLIVLGLLVLVSA
 1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-56
55 - 106 52
IRD-478
31 - 80 50
IRD-496
88 - 140 53
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.271
1.8 -6.8 -12.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
P01080 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 M15186
X03778
X04118
REFERENCES
PubMed ID:     16593809    | 3016659    | 7074039    | 2494344    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-2 CPLNC Chymotrypsin 91 - 95
RL-2 CPLNC Chymotrypsin 91 - 95
RL-48 CIREC Trypsin 34 - 38
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves Wounding transgenic in fusion with chloramphenicol acetyltransferase gene 16593809
Gene structure 3016659
Protein sequence determination 7074039
Crystal structure 2494344
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
trypsin, chymotrypsin 7074039
Streptomyces griseus proteinase B 2494344
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
1 4 4 2494344
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-56 55 - 106 CPLNC 90 - 94 DPKKP 83 - 87 H-L Type (Type-I)
IRD-478 31 - 80 CIREC 33 - 37 EGSPE 49 - 53 L-H Type (Type-II)
IRD-496 88 - 140 CPLNC 90 - 94 - H+L Type (Type-III)
PICTNCCAGYKGCNYYSANGAFICEGQSDPKKPKACPLNCDPHIAYSKCPRS
CPLNC
DPKKP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
P I C T N C C A G Y K G C N Y Y S A N G A F I C E G Q S D P K K P K A C P L N C D P H I A Y S K C P R S


KACIRECGNLGFGICPRSEGSPENPICTNCCAGYKGCNYYSANGAFICEG
CIREC
EGSPE
L-H Type (Type-II)
3,27 ; 7,31 ; 15,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C I R E C G N L G F G I C P R S E G S P E N P I C T N C C A G Y K G C N Y Y S A N G A F I C E G


KACPLNCDPHIAYSKCPRSEGKSLIYPTGCTTCCTGYKGCYYFGKNGKFVCEG
CPLNC
H+L Type (Type-III)
3,33 ; 7,34 ; 16,30 ; 40,51
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53
K A C P L N C D P H I A Y S K C P R S E G K S L I Y P T G C T T C C T G Y K G C Y Y F G K N G K F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-56
 55 - 106 52
IRD-478
 31 - 80 50
IRD-496
 88 - 140 53
Amino Acid Count % by Frequency
Alanine 11 7.19
Cysteine 16 10.46
Aspartic acid 6 3.92
Glutamic acid 9 5.88
Phenylalanine 5 3.27
Glycine 16 10.46
Histidine 2 1.31
Isoleucine 7 4.58
Lysine 12 7.84
Leucine 9 5.88
Methionine 4 2.61
Asparagine 9 5.88
Proline 11 7.19
Glutamine 1 0.65
Arginine 3 1.96
Serine 9 5.88
Threonine 5 3.27
Valine 8 5.23
Tryptophan 0 0
Tyrosine 10 6.54