Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_301 [A0A1J6J0L2]  : Wound-induced proteinase inhibitor 2

339Length

Nicotiana attenuataSpecie

PIN2_1 A4A49_16286Gene Name

37614Mass

9Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSML VSNVEHADAK ACPRNCDGRI AYEICPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFICEG ESDPRNPKAC PRNCDGRIAY GICPRSEEKK NDRICTNCCA GTKGCKYFSD DGTFICEGES 140
DPRNPKACPR NCDGRIAYEI CPRSEEKKND RICTNCCTGT KGCKYFSDDG TFVCEGESDP RNPKACPRNC 210
DGRIAYEICP RTEEKKNDRI CTNCCAGTKG CKYFSDDGTF VCEGESDPRN PKACPRNCDE RIAYGICPRT 280
EEKKNNQICT NCCAGTKGCN YFSANGTFIC EGESEYVSKV DEYVHEVEND LQKSRVAVS 339
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSMLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-165
55 - 106 52
IRD-166
113 - 164 52
IRD-169
229 - 280 52
IRD-214
171 - 222 52
IRD-615
30 - 80 51
IRD-616
88 - 138 51
IRD-617
146 - 196 51
IRD-618
204 - 254 51
IRD-619
262 - 312 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.8
2.1 -19 -33.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1J6J0L2 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 MJEQ01037185
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-165 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-166 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-169 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-214 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-615 30 - 80 CPRNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-616 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-617 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-618 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-619 262 - 312 CPRNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCKYFSDDGTFICEGESDPRNPKACPRNCDGRIAYEICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F I C E G E S D P R N P K A C P R N C D G R I A Y E I C P R S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDERIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D E R I A Y G I C P R T


RICTNCCTGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYEICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C T G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y E I C P R T


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F I C E G


KACPRNCDGRIAYEICPRSEEKKNDRICTNCCTGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R S E E K K N D R I C T N C C T G T K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYEICPRTEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y E I C P R T E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACPRNCDERIAYGICPRTEEKKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D E R I A Y G I C P R T E E K K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-165
 55 - 106 52
IRD-166
 113 - 164 52
IRD-169
 229 - 280 52
IRD-214
 171 - 222 52
IRD-615
 30 - 80 51
IRD-616
 88 - 138 51
IRD-617
 146 - 196 51
IRD-618
 204 - 254 51
IRD-619
 262 - 312 51
Amino Acid Count % by Frequency
Alanine 20 5.9
Cysteine 40 11.8
Aspartic acid 24 7.08
Glutamic acid 29 8.55
Phenylalanine 12 3.54
Glycine 27 7.96
Histidine 3 0.88
Isoleucine 18 5.31
Lysine 26 7.67
Leucine 7 2.06
Methionine 3 0.88
Asparagine 24 7.08
Proline 18 5.31
Glutamine 2 0.59
Arginine 25 7.37
Serine 19 5.6
Threonine 18 5.31
Valine 12 3.54
Tryptophan 0 0
Tyrosine 12 3.54