Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_302 [E9JGT0]  : Serine proteinase inhibitor 2c-R2

155Length

Solanum nigrumSpecie

SPI2c-R2Gene Name

16978Mass

3Domain Count

Sequence :
     FASTA
MAIYKVALLL LFGMIVLGSN ADGKPCTREC DTRIAYGICP LSETKRINQI CTNCCAGKKG CMYFSNDGTF 70
ICEGESEYVS EVPVDNKPCP RNCDGRIAYG ICPLSENKRI NQICTNCCAG KKGCLYFSND GTFICEGESE 140
YVSENDLQKI KVAIS 155
SIGNAL PEPTIDE
SEQUENCE Position
MAIYKVALLLLFGMIVLGSNA
 1 ↔ 21
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-3
49 - 105 57
IRD-516
87 - 137 51
IRD-526
24 - 74 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.093
-0.1 -7.8 -13.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
E9JGT0 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 GU133372
REFERENCES
PubMed ID:     21177479    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 90 - 94
RL-1 CPRNC Trypsin 90 - 94
RL-4 CTREC Trypsin 27 - 31
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves,Flowers Wounding, Oral secretion Oral secretion,Methyl Jasmonate Manduca sexta 21177479
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-3 49 - 105 CPRNC 89 - 93 - H+L Type (Type-III)
IRD-516 87 - 137 CPRNC 89 - 93 - H+L Type (Type-III)
IRD-526 24 - 74 CTREC 26 - 30 ETKRI 43 - 47 L-H Type (Type-II)
QICTNCCAGKKGCMYFSNDGTFICEGESEYVSEVPVDNKPCPRNCDGRIAYGICPLS
CPRNC
H+L Type (Type-III)
3,45 ; 6,24 ; 7,41 ; 13,54
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57
Q I C T N C C A G K K G C M Y F S N D G T F I C E G E S E Y V S E V P V D N K P C P R N C D G R I A Y G I C P L S


KPCPRNCDGRIAYGICPLSENKRINQICTNCCAGKKGCLYFSNDGTFICEG
CPRNC
H+L Type (Type-III)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P L S E N K R I N Q I C T N C C A G K K G C L Y F S N D G T F I C E G


KPCTRECDTRIAYGICPLSETKRINQICTNCCAGKKGCMYFSNDGTFICEG
CTREC
ETKRI
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T R E C D T R I A Y G I C P L S E T K R I N Q I C T N C C A G K K G C M Y F S N D G T F I C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-3
 49 - 105 57
IRD-516
 87 - 137 51
IRD-526
 24 - 74 51
Amino Acid Count % by Frequency
Alanine 8 5.16
Cysteine 16 10.32
Aspartic acid 7 4.52
Glutamic acid 11 7.1
Phenylalanine 5 3.23
Glycine 14 9.03
Histidine 0 0
Isoleucine 14 9.03
Lysine 11 7.1
Leucine 9 5.81
Methionine 3 1.94
Asparagine 11 7.1
Proline 6 3.87
Glutamine 3 1.94
Arginine 6 3.87
Serine 10 6.45
Threonine 7 4.52
Valine 7 4.52
Tryptophan 0 0
Tyrosine 7 4.52