Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_305 [Q1WL31]  : Trypsin proteinase inhibitor

516Length

Nicotiana obtusifoliaSpecie

PIGene Name

56513Mass

15Domain Count

Sequence :
     FASTA
MAAHRVSFLA LLPLFGMSLL VSIVERMSLL VSIVEHADAK ACPRNCDGRI AYGICPRSEE KKNDRICTNC 70
CAGMKGCNYF SADGTFVCEG ESDPINPKAC PFNCDPRIAY GVCPRSEEKK NNQICTNCCA GRKGCYYFSA 140
DGAFVCEGES DPKNPKACPR NCDGRIAFKV CPRSEEKKND RICTNCCAGR KGCSYFSADG TFVCKGESDP 210
RNPKACPRNC DPRIDFGICP LSEEKKDNQI CTNCYAGMKG CYYFSADGTF VCEGESDPRN PKPCPRNCDG 280
RIAYGICPRS EEKKNDRICT NCCAGTKGCS YFSADGTFVC KGESDPRNPK ACPRNCDPRI DFGICPLSEE 350
KKDNQICTNC CAGMKGCNYF SADGTFVCEG ESDPRNPKPC PRNCDGRIAY GICPRSEEKK NDRICTNCCA 420
GTKGCSYFSA DGTFVCKGES DPRNPKACPR NCDPRIDFGI CPLLEEKKDN QICTNCCAGM KGCNYFSANG 490
TFICEGESEY VNKVENEHQM SKVAVI 516
SIGNAL PEPTIDE
SEQUENCE Position
MAAHRVSFLALLPLFGMS
 1 ↔ 18
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-61
65 - 116 52
IRD-154
123 - 174 52
IRD-155
181 - 232 52
IRD-156
239 - 290 52
IRD-157
297 - 348 52
IRD-158
355 - 406 52
IRD-159
413 - 464 52
IRD-591
214 - 264 51
IRD-592
330 - 380 51
IRD-593
446 - 496 51
IRD-607
40 - 90 51
IRD-608
156 - 206 51
IRD-609
388 - 438 51
IRD-609
272 - 322 51
IRD-665
98 - 148 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.815
9.5 -21.7 -44.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL31 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158201
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 43 - 47
RL-1 CPRNC Trypsin 159 - 163
RL-1 CPRNC Trypsin 159 - 163
RL-1 CPRNC Trypsin 217 - 221
RL-1 CPRNC Trypsin 217 - 221
RL-1 CPRNC Trypsin 275 - 279
RL-1 CPRNC Trypsin 275 - 279
RL-1 CPRNC Trypsin 333 - 337
RL-1 CPRNC Trypsin 333 - 337
RL-1 CPRNC Trypsin 391 - 395
RL-1 CPRNC Trypsin 391 - 395
RL-1 CPRNC Trypsin 449 - 453
RL-1 CPRNC Trypsin 449 - 453
RL-5 CPFNC Chymotrypsin 101 - 105
RL-5 CPFNC Chymotrypsin 101 - 105
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-61 65 - 116 CPFNC 100 - 104 DPINP 93 - 97 H-L Type (Type-I)
IRD-154 123 - 174 CPRNC 158 - 162 DPKNP 151 - 155 H-L Type (Type-I)
IRD-155 181 - 232 CPRNC 216 - 220 DPRNP 209 - 213 H-L Type (Type-I)
IRD-156 239 - 290 CPRNC 274 - 278 DPRNP 267 - 271 H-L Type (Type-I)
IRD-157 297 - 348 CPRNC 332 - 336 DPRNP 325 - 329 H-L Type (Type-I)
IRD-158 355 - 406 CPRNC 390 - 394 DPRNP 383 - 387 H-L Type (Type-I)
IRD-159 413 - 464 CPRNC 448 - 452 DPRNP 441 - 445 H-L Type (Type-I)
IRD-591 214 - 264 CPRNC 216 - 220 EEKKD 233 - 237 L-H Type (Type-II)
IRD-592 330 - 380 CPRNC 332 - 336 EEKKD 349 - 353 L-H Type (Type-II)
IRD-593 446 - 496 CPRNC 448 - 452 EEKKD 465 - 469 L-H Type (Type-II)
IRD-607 40 - 90 CPRNC 42 - 46 EEKKN 59 - 63 L-H Type (Type-II)
IRD-608 156 - 206 CPRNC 158 - 162 EEKKN 175 - 179 L-H Type (Type-II)
IRD-609 388 - 438 CPRNC 390 - 394 EEKKN 407 - 411 L-H Type (Type-II)
IRD-609 272 - 322 CPRNC 274 - 278 EEKKN 291 - 295 L-H Type (Type-II)
IRD-665 98 - 148 CPFNC 100 - 104 EEKKN 117 - 121 L-H Type (Type-II)
RICTNCCAGMKGCNYFSADGTFVCEGESDPINPKACPFNCDPRIAYGVCPRS
CPFNC
DPINP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E S D P I N P K A C P F N C D P R I A Y G V C P R S


QICTNCCAGRKGCYYFSADGAFVCEGESDPKNPKACPRNCDGRIAFKVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G R K G C Y Y F S A D G A F V C E G E S D P K N P K A C P R N C D G R I A F K V C P R S


RICTNCCAGRKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L S


QICTNCYAGMKGCYYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
24,49 ; 36,40
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C Y A G M K G C Y Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L S


QICTNCCAGMKGCNYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G M K G C N Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLL
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L L


KACPRNCDPRIDFGICPLSEEKKDNQICTNCYAGMKGCYYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,38 ; 16,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L S E E K K D N Q I C T N C Y A G M K G C Y Y F S A D G T F V C E G


KACPRNCDPRIDFGICPLSEEKKDNQICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L S E E K K D N Q I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDPRIDFGICPLLEEKKDNQICTNCCAGMKGCNYFSANGTFICEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L L E E K K D N Q I C T N C C A G M K G C N Y F S A N G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAFKVCPRSEEKKNDRICTNCCAGRKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A F K V C P R S E E K K N D R I C T N C C A G R K G C S Y F S A D G T F V C K G


KPCPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C S Y F S A D G T F V C K G


KPCPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPFNCDPRIAYGVCPRSEEKKNNQICTNCCAGRKGCYYFSADGAFVCEG
CPFNC
EEKKN
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G R K G C Y Y F S A D G A F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-61
 65 - 116 52
IRD-154
 123 - 174 52
IRD-155
 181 - 232 52
IRD-156
 239 - 290 52
IRD-157
 297 - 348 52
IRD-158
 355 - 406 52
IRD-159
 413 - 464 52
IRD-591
 214 - 264 51
IRD-592
 330 - 380 51
IRD-593
 446 - 496 51
IRD-607
 40 - 90 51
IRD-608
 156 - 206 51
IRD-609
 388 - 438 51
IRD-609
 272 - 322 51
IRD-665
 98 - 148 51
Amino Acid Count % by Frequency
Alanine 34 6.59
Cysteine 63 12.21
Aspartic acid 33 6.4
Glutamic acid 34 6.59
Phenylalanine 23 4.46
Glycine 44 8.53
Histidine 3 0.58
Isoleucine 27 5.23
Lysine 39 7.56
Leucine 12 2.33
Methionine 8 1.55
Asparagine 38 7.36
Proline 37 7.17
Glutamine 5 0.97
Arginine 33 6.4
Serine 32 6.2
Threonine 17 3.29
Valine 18 3.49
Tryptophan 0 0
Tyrosine 16 3.1