Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_308 [Q1WL35]  : Trypsin proteinase inhibitor

506Length

Nicotiana obtusifoliaSpecie

PIGene Name

55385Mass

15Domain Count

Sequence :
     FASTA
MAAHRVSFLA LLPLFGMSLL VSIVEHADAK ACPRNCDGRI AYGICPRSEE KKNDRICTNC CAGMKGCNYF 70
SADGTFVCEG ESDPINPKAC PFNCDPRIAY GVCPRSEEKK NNQICTNCCA GRKGCYYFSA DGAFVCEGES 140
DPKNPKACPR NCDGRIAFKV CPRSEEKKND RICTNCCAGR KGCSYFSADG TFVCKGESDP RNPKACPRNC 210
DPRIDFGICP LSEEKKDNQI CTNCYAGMKG CYYFSADGTF VCEGESDPRN PKPCPRNCDG RIAYGICPRS 280
EEKKNDRICT NCCAGTKGCS YFSADGTFVC KGESDPRNPK ACPRNCDPRI DFGICPLSEE KKDNQICTNC 350
CAGMKGCNYF SADGTFVCEG ESDPRNPKPC PRNCDGRIAY GICPRSEEKK NDRICTNCCA GTKGCSYFSA 420
DGTFVCKGES DPRNPKACPR NCDPRIDFGI CPLLEEKKDN QICTNCCAGM KGCNYFSANG TFICEGESEY 490
VNKVENEHQM SKVAVI 506
SIGNAL PEPTIDE
SEQUENCE Position
MAAHRVSFLALLPLFGMSLLVSIVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-61
55 - 106 52
IRD-154
113 - 164 52
IRD-155
171 - 222 52
IRD-156
229 - 280 52
IRD-157
287 - 338 52
IRD-158
345 - 396 52
IRD-159
403 - 454 52
IRD-591
204 - 254 51
IRD-592
320 - 370 51
IRD-593
436 - 486 51
IRD-607
30 - 80 51
IRD-608
146 - 196 51
IRD-609
378 - 428 51
IRD-609
262 - 312 51
IRD-665
88 - 138 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.81
9.4 -21.7 -44.6
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL35 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158197
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 33 - 37
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 381 - 385
RL-1 CPRNC Trypsin 381 - 385
RL-1 CPRNC Trypsin 439 - 443
RL-1 CPRNC Trypsin 439 - 443
RL-5 CPFNC Chymotrypsin 91 - 95
RL-5 CPFNC Chymotrypsin 91 - 95
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-61 55 - 106 CPFNC 90 - 94 DPINP 83 - 87 H-L Type (Type-I)
IRD-154 113 - 164 CPRNC 148 - 152 DPKNP 141 - 145 H-L Type (Type-I)
IRD-155 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-156 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-157 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-158 345 - 396 CPRNC 380 - 384 DPRNP 373 - 377 H-L Type (Type-I)
IRD-159 403 - 454 CPRNC 438 - 442 DPRNP 431 - 435 H-L Type (Type-I)
IRD-591 204 - 254 CPRNC 206 - 210 EEKKD 223 - 227 L-H Type (Type-II)
IRD-592 320 - 370 CPRNC 322 - 326 EEKKD 339 - 343 L-H Type (Type-II)
IRD-593 436 - 486 CPRNC 438 - 442 EEKKD 455 - 459 L-H Type (Type-II)
IRD-607 30 - 80 CPRNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-608 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-609 378 - 428 CPRNC 380 - 384 EEKKN 397 - 401 L-H Type (Type-II)
IRD-609 262 - 312 CPRNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
IRD-665 88 - 138 CPFNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
RICTNCCAGMKGCNYFSADGTFVCEGESDPINPKACPFNCDPRIAYGVCPRS
CPFNC
DPINP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E S D P I N P K A C P F N C D P R I A Y G V C P R S


QICTNCCAGRKGCYYFSADGAFVCEGESDPKNPKACPRNCDGRIAFKVCPRS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G R K G C Y Y F S A D G A F V C E G E S D P K N P K A C P R N C D G R I A F K V C P R S


RICTNCCAGRKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L S


QICTNCYAGMKGCYYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
24,49 ; 36,40
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C Y A G M K G C Y Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L S


QICTNCCAGMKGCNYFSADGTFVCEGESDPRNPKPCPRNCDGRIAYGICPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G M K G C N Y F S A D G T F V C E G E S D P R N P K P C P R N C D G R I A Y G I C P R S


RICTNCCAGTKGCSYFSADGTFVCKGESDPRNPKACPRNCDPRIDFGICPLL
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C S Y F S A D G T F V C K G E S D P R N P K A C P R N C D P R I D F G I C P L L


KACPRNCDPRIDFGICPLSEEKKDNQICTNCYAGMKGCYYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,38 ; 16,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L S E E K K D N Q I C T N C Y A G M K G C Y Y F S A D G T F V C E G


KACPRNCDPRIDFGICPLSEEKKDNQICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L S E E K K D N Q I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDPRIDFGICPLLEEKKDNQICTNCCAGMKGCNYFSANGTFICEG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I D F G I C P L L E E K K D N Q I C T N C C A G M K G C N Y F S A N G T F I C E G


KACPRNCDGRIAYGICPRSEEKKNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAFKVCPRSEEKKNDRICTNCCAGRKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,32 ; 7,38 ; 16,28 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A F K V C P R S E E K K N D R I C T N C C A G R K G C S Y F S A D G T F V C K G


KPCPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C S Y F S A D G T F V C K G


KPCPRNCDGRIAYGICPRSEEKKNDRICTNCCAGTKGCSYFSADGTFVCKG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D G R I A Y G I C P R S E E K K N D R I C T N C C A G T K G C S Y F S A D G T F V C K G


KACPFNCDPRIAYGVCPRSEEKKNNQICTNCCAGRKGCYYFSADGAFVCEG
CPFNC
EEKKN
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R S E E K K N N Q I C T N C C A G R K G C Y Y F S A D G A F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-61
 55 - 106 52
IRD-154
 113 - 164 52
IRD-155
 171 - 222 52
IRD-156
 229 - 280 52
IRD-157
 287 - 338 52
IRD-158
 345 - 396 52
IRD-159
 403 - 454 52
IRD-591
 204 - 254 51
IRD-592
 320 - 370 51
IRD-593
 436 - 486 51
IRD-607
 30 - 80 51
IRD-608
 146 - 196 51
IRD-609
 378 - 428 51
IRD-609
 262 - 312 51
IRD-665
 88 - 138 51
Amino Acid Count % by Frequency
Alanine 34 6.72
Cysteine 63 12.45
Aspartic acid 33 6.52
Glutamic acid 33 6.52
Phenylalanine 23 4.55
Glycine 44 8.7
Histidine 3 0.59
Isoleucine 26 5.14
Lysine 39 7.71
Leucine 10 1.98
Methionine 7 1.38
Asparagine 38 7.51
Proline 37 7.31
Glutamine 5 0.99
Arginine 32 6.32
Serine 30 5.93
Threonine 17 3.36
Valine 16 3.16
Tryptophan 0 0
Tyrosine 16 3.16