Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_316 [A0A1S4A0N1]  : wound-induced proteinase inhibitor 2-like

396Length

Nicotiana tabacumSpecie

LOC107792454Gene Name

43323Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LVLLFGMSLL VSNVEHADAK ACPFNCDPRI AYGVCPRLEE DKNNQICTNC CAGTKGCNYF 70
SADGTFICKG QSDPRNPKAC PRNCDPRIAY GVCPRLEEKK DNQICTNCCA GTKGCNYFSA DGTFVCKGQS 140
DPRNPKACPR NCDPRIAYGV CPRSEEKKDN QICTNCCAGT KGCNYFSADG TFVCKGQSDP RNPKACPRNC 210
DPRIDYGVCP RSEEKDNQIC TNCCAGTKGC NYFSADGTFV CKGQSDPRNP KACPRNCDPR IAYRVCPRSE 280
EKEDNQICTN CCAGTKGCNY FSANGTFVCK GQSDPRNPKA CPRNCDPRIA YGVCPRSEEE KNNQICTNCC 350
GGTKGCNYFS ANGTFICEGE SEYVSKVDEY VREVENDLQK SKVAVS 396
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALVLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-206
55 - 106 52
IRD-207
113 - 164 52
IRD-208
171 - 222 52
IRD-209
228 - 279 52
IRD-210
286 - 337 52
IRD-401
30 - 80 51
IRD-531
261 - 311 51
IRD-573
204 - 253 50
IRD-586
319 - 369 51
IRD-597
88 - 138 51
IRD-598
146 - 196 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.109
10.9 -12.3 -29.7
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1S4A0N1 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     24807620    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 264 - 268
RL-1 CPRNC Trypsin 322 - 326
RL-1 CPRNC Trypsin 322 - 326
RL-5 CPFNC Chymotrypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Genome annotation 24807620
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-206 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-207 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-208 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-209 228 - 279 CPRNC 263 - 267 DPRNP 256 - 260 H-L Type (Type-I)
IRD-210 286 - 337 CPRNC 321 - 325 DPRNP 314 - 318 H-L Type (Type-I)
IRD-401 30 - 80 CPFNC 32 - 36 EEDKN 49 - 53 L-H Type (Type-II)
IRD-531 261 - 311 CPRNC 263 - 267 EKEDN 281 - 285 L-H Type (Type-II)
IRD-573 204 - 253 CPRNC 206 - 210 EEKDN 223 - 227 L-H Type (Type-II)
IRD-586 319 - 369 CPRNC 321 - 325 EEKNN 339 - 343 L-H Type (Type-II)
IRD-597 88 - 138 CPRNC 90 - 94 EEKKD 107 - 111 L-H Type (Type-II)
IRD-598 146 - 196 CPRNC 148 - 152 EEKKD 165 - 169 L-H Type (Type-II)
QICTNCCAGTKGCNYFSADGTFICKGQSDPRNPKACPRNCDPRIAYGVCPRL
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F I C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R L


QICTNCCAGTKGCNYFSADGTFVCKGQSDPRNPKACPRNCDPRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGQSDPRNPKACPRNCDPRIDYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G Q S D P R N P K A C P R N C D P R I D Y G V C P R S


QICTNCCAGTKGCNYFSADGTFVCKGQSDPRNPKACPRNCDPRIAYRVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A D G T F V C K G Q S D P R N P K A C P R N C D P R I A Y R V C P R S


QICTNCCAGTKGCNYFSANGTFVCKGQSDPRNPKACPRNCDPRIAYGVCPRS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F V C K G Q S D P R N P K A C P R N C D P R I A Y G V C P R S


KACPFNCDPRIAYGVCPRLEEDKNNQICTNCCAGTKGCNYFSADGTFICKG
CPFNC
EEDKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P F N C D P R I A Y G V C P R L E E D K N N Q I C T N C C A G T K G C N Y F S A D G T F I C K G


KACPRNCDPRIAYRVCPRSEEKEDNQICTNCCAGTKGCNYFSANGTFVCKG
CPRNC
EKEDN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y R V C P R S E E K E D N Q I C T N C C A G T K G C N Y F S A N G T F V C K G


KACPRNCDPRIDYGVCPRSEEKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKDN
L-H Type (Type-II)
3,27 ; 7,31 ; 16,37 ; 30,48
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50
K A C P R N C D P R I D Y G V C P R S E E K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGVCPRSEEEKNNQICTNCCGGTKGCNYFSANGTFICEG
CPRNC
EEKNN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E E K N N Q I C T N C C G G T K G C N Y F S A N G T F I C E G


KACPRNCDPRIAYGVCPRLEEKKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R L E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


KACPRNCDPRIAYGVCPRSEEKKDNQICTNCCAGTKGCNYFSADGTFVCKG
CPRNC
EEKKD
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G V C P R S E E K K D N Q I C T N C C A G T K G C N Y F S A D G T F V C K G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-206
 55 - 106 52
IRD-207
 113 - 164 52
IRD-208
 171 - 222 52
IRD-209
 228 - 279 52
IRD-210
 286 - 337 52
IRD-401
 30 - 80 51
IRD-531
 261 - 311 51
IRD-573
 204 - 253 50
IRD-586
 319 - 369 51
IRD-597
 88 - 138 51
IRD-598
 146 - 196 51
Amino Acid Count % by Frequency
Alanine 27 6.82
Cysteine 48 12.12
Aspartic acid 24 6.06
Glutamic acid 21 5.3
Phenylalanine 15 3.79
Glycine 31 7.83
Histidine 2 0.51
Isoleucine 14 3.54
Lysine 28 7.07
Leucine 9 2.27
Methionine 2 0.51
Asparagine 35 8.84
Proline 28 7.07
Glutamine 12 3.03
Arginine 25 6.31
Serine 22 5.56
Threonine 18 4.55
Valine 21 5.3
Tryptophan 0 0
Tyrosine 14 3.54