PINIR_357 [T1PZ89]  : Pin-II type proteinase inhibitor 53

204Length

Capsicum annuumSpecie

-Gene Name

22310Mass

5Domain Count

Sequence :
    FASTA
MAVPKEVSFL AFLLVLEILL LHVDAKACSE ENAENRICTN CCAGRKGCNY YSADGTFICE GESDPNNPKA 70
CPRNCDTRIA YSKCPRSEGN AENRLCTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKPCP RNCDTRIAYS 140
ICPRSEGNAE NRICTNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDPNIAYSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFLLVLEILLLHVDA
1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
36 - 87 52
94 - 145 52
152 - 203 52
69 - 119 51
127 - 177 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.939
-1.2 -13.5 -22.3
CROSS REFERENCES
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 72 - 76
RL-1 CPRNC Trypsin 72 - 76
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-83 36 - 87 CPRNC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-92 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-93 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-536 69 - 119 CPRNC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
IRD-541 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)

RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D T R I A Y S K C P R S



RLCTNCCAGRKGCNYYSADGTFICEGESDPNNPKPCPRNCDTRIAYSICPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K P C P R N C D T R I A Y S I C P R S



RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N I A Y S L C L Y E



KACPRNCDTRIAYSKCPRSEGNAENRLCTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y S K C P R S E G N A E N R L C T N C C A G R K G C N Y Y S A D G T F I C E G



KPCPRNCDTRIAYSICPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D T R I A Y S I C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G



DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-83
36 - 87 52
IRD-92
94 - 145 52
IRD-93
152 - 203 52
IRD-536
69 - 119 51
IRD-541
127 - 177 51
Amino Acid Count % by Frequency
Alanine 18 8.82
Cysteine 25 12.25
Aspartic acid 10 4.9
Glutamic acid 16 7.84
Phenylalanine 5 2.45
Glycine 14 6.86
Histidine 1 0.49
Isoleucine 10 4.9
Lysine 10 4.9
Leucine 10 4.9
Methionine 1 0.49
Asparagine 22 10.78
Proline 14 6.86
Glutamine 0 0
Arginine 13 6.37
Serine 13 6.37
Threonine 8 3.92
Valine 4 1.96
Tryptophan 0 0
Tyrosine 10 4.9