PINIR_358 [T1PZK2]  : Pin-II type proteinase inhibitor 52

204Length

Capsicum annuumSpecie

-Gene Name

22313Mass

5Domain Count

Sequence :
    FASTA
MAVPKEVSFL AFLLVLEILL LHVDAKACSE ENAENRICTN CCAGRKGCNY YSADGTFICE GESDPNNPKT 70
CPRYCDTRIA YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKACP RNCDTRIAYS 140
KCPRSEGNAE NRLCTNCCAG RKGCNYHSAD GTFICEGESD PNTPKACPRN CDPNIAHSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFLLVLEILLLHVDA
1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
94 - 145 52
152 - 203 52
36 - 87 52
127 - 177 51
69 - 119 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.661
1 -12.5 -21.4
CROSS REFERENCES
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
RL-6 CPRYC Trypsin 72 - 76
RL-6 CPRYC Trypsin 72 - 76
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-83 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-107 152 - 203 CPRNC 187 - 191 DPNTP 180 - 184 H-L Type (Type-I)
IRD-139 36 - 87 CPRYC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-548 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
IRD-562 69 - 119 CPRYC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)

RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D T R I A Y S K C P R S



RLCTNCCAGRKGCNYHSADGTFICEGESDPNTPKACPRNCDPNIAHSLCLYE
CPRNC
DPNTP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y H S A D G T F I C E G E S D P N T P K A C P R N C D P N I A H S L C L Y E



RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKTCPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K T C P R Y C D T R I A Y S K C P R S



KACPRNCDTRIAYSKCPRSEGNAENRLCTNCCAGRKGCNYHSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y S K C P R S E G N A E N R L C T N C C A G R K G C N Y H S A D G T F I C E G



KTCPRYCDTRIAYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRYC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K T C P R Y C D T R I A Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G



DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-83
94 - 145 52
IRD-107
152 - 203 52
IRD-139
36 - 87 52
IRD-548
127 - 177 51
IRD-562
69 - 119 51
Amino Acid Count % by Frequency
Alanine 18 8.82
Cysteine 25 12.25
Aspartic acid 10 4.9
Glutamic acid 16 7.84
Phenylalanine 5 2.45
Glycine 14 6.86
Histidine 3 1.47
Isoleucine 9 4.41
Lysine 11 5.39
Leucine 10 4.9
Methionine 1 0.49
Asparagine 20 9.8
Proline 13 6.37
Glutamine 0 0
Arginine 13 6.37
Serine 13 6.37
Threonine 10 4.9
Valine 4 1.96
Tryptophan 0 0
Tyrosine 9 4.41