PINIR_370 [T1PZK4]  : Pin-II type proteinase inhibitor 57

204Length

Capsicum annuumSpecie

-Gene Name

22442Mass

5Domain Count

Sequence :
    FASTA
MAVPKEVSFL AFVLVLGILL LHVDAKACSE ENAENRICTN CCAGRKGCNY YSADGTFICE GESDPNNSKP 70
CTLNCDPRIF YSKCPRSEGN AENRICTNCC AGRKGCYYYS DDGTFICEGE SDPNNPKPCP RNCDTRIAYS 140
ICPRSEGNAE NRICTNCCAG RNFCNYYSAD GTFICEGESD PNNPKACPRN CDPNIAYSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFVLVLGILLLHVDA
1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
36 - 87 52
94 - 145 52
152 - 203 52
127 - 177 51
69 - 119 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.616
-3.3 -15.5 -24.2
CROSS REFERENCES
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
RL-3 CTLNC Chymotrypsin 72 - 76
RL-3 CTLNC Chymotrypsin 72 - 76
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-60 36 - 87 CTLNC 71 - 75 DPNNS 64 - 68 H-L Type (Type-I)
IRD-103 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-117 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-549 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)
IRD-552 69 - 119 CTLNC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)

RICTNCCAGRKGCNYYSADGTFICEGESDPNNSKPCTLNCDPRIFYSKCPRS
CTLNC
DPNNS
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N S K P C T L N C D P R I F Y S K C P R S



RICTNCCAGRKGCYYYSDDGTFICEGESDPNNPKPCPRNCDTRIAYSICPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C Y Y Y S D D G T F I C E G E S D P N N P K P C P R N C D T R I A Y S I C P R S



RICTNCCAGRNFCNYYSADGTFICEGESDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R N F C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N I A Y S L C L Y E



KPCPRNCDTRIAYSICPRSEGNAENRICTNCCAGRNFCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C P R N C D T R I A Y S I C P R S E G N A E N R I C T N C C A G R N F C N Y Y S A D G T F I C E G



KPCTLNCDPRIFYSKCPRSEGNAENRICTNCCAGRKGCYYYSDDGTFICEG
CTLNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K P C T L N C D P R I F Y S K C P R S E G N A E N R I C T N C C A G R K G C Y Y Y S D D G T F I C E G



DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-60
36 - 87 52
IRD-103
94 - 145 52
IRD-117
152 - 203 52
IRD-549
127 - 177 51
IRD-552
69 - 119 51
Amino Acid Count % by Frequency
Alanine 15 7.35
Cysteine 25 12.25
Aspartic acid 11 5.39
Glutamic acid 15 7.35
Phenylalanine 7 3.43
Glycine 14 6.86
Histidine 1 0.49
Isoleucine 11 5.39
Lysine 9 4.41
Leucine 9 4.41
Methionine 1 0.49
Asparagine 22 10.78
Proline 14 6.86
Glutamine 0 0
Arginine 12 5.88
Serine 14 6.86
Threonine 8 3.92
Valine 5 2.45
Tryptophan 0 0
Tyrosine 11 5.39