PINIR_391 [T1PZB7]  : Pin-II type proteinase inhibitor 64

204Length

Capsicum annuumSpecie

-Gene Name

22164Mass

5Domain Count

Sequence :
    FASTA
MAVPKEVSFL AFVLVLEILL VHVDAKACSE ENAENGICTN CCAGRKGCNY YSADGTFICE GESDPNNPKA 70
CPRYCDTRIA YSKCPRSEGG AENRLCTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKACP RNCDTRIAYS 140
KCPRSEGNAE NRLCTNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDPNIAYSLC LYEK 204
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFVLVLEILLVHVDA
1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
152 - 203 52
94 - 145 52
36 - 87 52
69 - 119 51
127 - 177 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.937
-1.2 -13.5 -22.4
CROSS REFERENCES
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 130 - 134
RL-1 CPRNC Trypsin 188 - 192
RL-6 CPRYC Trypsin 72 - 76
RL-6 CPRYC Trypsin 72 - 76
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
Flowers,stems,fruit,leaves Wounding Insect Oral secretion 23153298
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-84 152 - 203 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-90 94 - 145 CPRNC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-136 36 - 87 CPRYC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-404 69 - 119 CPRYC 71 - 75 EGGAE 88 - 92 L-H Type (Type-II)
IRD-536 127 - 177 CPRNC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)

RLCTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDPNIAYSLCLYE
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N I A Y S L C L Y E



RLCTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDTRIAYSKCPRS
CPRNC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R L C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D T R I A Y S K C P R S



GICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
G I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R Y C D T R I A Y S K C P R S



KACPRYCDTRIAYSKCPRSEGGAENRLCTNCCAGRKGCNYYSADGTFICEG
CPRYC
EGGAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R Y C D T R I A Y S K C P R S E G G A E N R L C T N C C A G R K G C N Y Y S A D G T F I C E G



KACPRNCDTRIAYSKCPRSEGNAENRLCTNCCAGRKGCNYYSADGTFICEG
CPRNC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y S K C P R S E G N A E N R L C T N C C A G R K G C N Y Y S A D G T F I C E G



DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-84
152 - 203 52
IRD-90
94 - 145 52
IRD-136
36 - 87 52
IRD-404
69 - 119 51
IRD-536
127 - 177 51
Amino Acid Count % by Frequency
Alanine 19 9.31
Cysteine 25 12.25
Aspartic acid 10 4.9
Glutamic acid 16 7.84
Phenylalanine 5 2.45
Glycine 16 7.84
Histidine 1 0.49
Isoleucine 8 3.92
Lysine 11 5.39
Leucine 9 4.41
Methionine 1 0.49
Asparagine 20 9.8
Proline 13 6.37
Glutamine 0 0
Arginine 12 5.88
Serine 13 6.37
Threonine 8 3.92
Valine 6 2.94
Tryptophan 0 0
Tyrosine 11 5.39