PINIR_401 [Q4U5Z3]  : PinII-type proteinase inhibitor 12 (Fragment)

220Length

Capsicum annuumSpecie

-Gene Name

24228Mass

5Domain Count

Sequence :
    FASTA
MAVPKEVSFL AFLLVLEILL LHVDAKACSE ENAENRICTN CCAGRKGCNY YSADGTFICE GESDPNNPKA 70
CPRYCDTRIA YSKCPRSEGN AENRICTNCC AGRKGCNYYS ADGTFICEGE SDPNNPKACP RYCDTRIAYS 140
KCPRSEGNAE NRLCPNCCAG RKGCNYYSAD GTFICEGESD PNNPKACPRN CDPNYCYLLP IHCVSMKSKS 210
METNFFGNSH 220
SIGNAL PEPTIDE
SEQUENCE Position
MAVPKEVSFLAFLLVLEILLLHVDA
1 ↔ 25
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
152 - 202 51
36 - 87 52
94 - 145 52
69 - 119 51
127 - 177 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
5.976
1.9 -11.9 -21.2
CROSS REFERENCES
REFERENCES
PubMed ID:    
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 188 - 192
RL-6 CPRYC Trypsin 72 - 76
RL-6 CPRYC Trypsin 72 - 76
RL-6 CPRYC Trypsin 130 - 134
RL-6 CPRYC Trypsin 130 - 134
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
leaves Helicoverpa armigera Show 60-80% reduction in protease activity,Show activity for trypsin and chymotrypsin, 35% reduction in larval weight 15715970
leaves Helicoverpa armigera Transgenic expression in tomato plant;IRD-9 and -12 exhibited strong inhibition (70–90%) of trypsin, chymotrypsin and HGP, while IRD-7 (40–70%) showed comparatively less inhibition 23261428
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-116 152 - 202 CPRNC 187 - 191 DPNNP 180 - 184 H-L Type (Type-I)
IRD-133 36 - 87 CPRYC 71 - 75 DPNNP 64 - 68 H-L Type (Type-I)
IRD-133 94 - 145 CPRYC 129 - 133 DPNNP 122 - 126 H-L Type (Type-I)
IRD-559 69 - 119 CPRYC 71 - 75 EGNAE 88 - 92 L-H Type (Type-II)
IRD-564 127 - 177 CPRYC 129 - 133 EGNAE 146 - 150 L-H Type (Type-II)

RLCPNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRNCDPNYCYLLPIH
CPRNC
DPNNP
H-L Type (Type-I)
3,36 ; 6,40 ; 7,24 ; 13,45
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
R L C P N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R N C D P N Y C Y L L P I H



RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R Y C D T R I A Y S K C P R S



RICTNCCAGRKGCNYYSADGTFICEGESDPNNPKACPRYCDTRIAYSKCPRS
CPRYC
DPNNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C N Y Y S A D G T F I C E G E S D P N N P K A C P R Y C D T R I A Y S K C P R S



KACPRYCDTRIAYSKCPRSEGNAENRICTNCCAGRKGCNYYSADGTFICEG
CPRYC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R Y C D T R I A Y S K C P R S E G N A E N R I C T N C C A G R K G C N Y Y S A D G T F I C E G



KACPRYCDTRIAYSKCPRSEGNAENRLCPNCCAGRKGCNYYSADGTFICEG
CPRYC
EGNAE
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R Y C D T R I A Y S K C P R S E G N A E N R L C P N C C A G R K G C N Y Y S A D G T F I C E G



DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-116
152 - 202 51
IRD-133
36 - 87 52
IRD-133
94 - 145 52
IRD-559
69 - 119 51
IRD-564
127 - 177 51
Amino Acid Count % by Frequency
Alanine 18 8.18
Cysteine 26 11.82
Aspartic acid 10 4.55
Glutamic acid 16 7.27
Phenylalanine 7 3.18
Glycine 15 6.82
Histidine 3 1.36
Isoleucine 9 4.09
Lysine 12 5.45
Leucine 10 4.55
Methionine 3 1.36
Asparagine 22 10
Proline 15 6.82
Glutamine 0 0
Arginine 13 5.91
Serine 16 7.27
Threonine 8 3.64
Valine 5 2.27
Tryptophan 0 0
Tyrosine 12 5.45