Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_49 [Q1WL44]  : Trypsin proteinase inhibitor

397Length

Nicotiana sylvestrisSpecie

PIGene Name

43559Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLLGMSLL VSNVEHADAK ACPLNCDPRI AYGVCPRSEE KKNDRICTNC CAGTKGCKYF 70
SDDGTFVCEG ESDPRNPKAC PRNCDPRIAY GICPLSEEKK NDRICTNCCA GTKGCKYFSD DGTFVCEGES 140
DPRNPKACPR NCDPRIAYGI CPLSEEKKND RICTNCCAGK KGCKYFSNDG TFVCEGESDP RNPKACTLNC 210
DPRIAYGICP LSEEKKNDRI CTNCCAGKKG CKYFSDDGTF VCEGESDPRN PKACTLNCDP RIAYGICPLS 280
EEKKNDRICT NCCAGKKGCK YFSDDGTFVC EGESDPRNPK ACPRNCDGRI AYGICPLSEE KKNDRICTNC 350
CAGKKGCKYF SDDGTFICEG ESEYASKVDE YVREVENDLQ KSKVAVS 397
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLLGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-152
113 - 164 52
IRD-152
55 - 106 52
IRD-153
287 - 338 52
IRD-254
171 - 222 52
IRD-255
229 - 280 52
IRD-604
88 - 138 51
IRD-606
320 - 370 51
IRD-620
146 - 196 51
IRD-655
30 - 80 51
IRD-658
204 - 254 51
IRD-658
262 - 312 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.182
3.6 -20.5 -38.4
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL44 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158188
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-2 CPLNC Chymotrypsin 33 - 37
RL-3 CTLNC Chymotrypsin 207 - 211
RL-3 CTLNC Chymotrypsin 207 - 211
RL-3 CTLNC Chymotrypsin 265 - 269
RL-3 CTLNC Chymotrypsin 265 - 269
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-152 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-152 55 - 106 CPRNC 90 - 94 DPRNP 83 - 87 H-L Type (Type-I)
IRD-153 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-254 171 - 222 CTLNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-255 229 - 280 CTLNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-604 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-606 320 - 370 CPRNC 322 - 326 EEKKN 339 - 343 L-H Type (Type-II)
IRD-620 146 - 196 CPRNC 148 - 152 EEKKN 165 - 169 L-H Type (Type-II)
IRD-655 30 - 80 CPLNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
IRD-658 204 - 254 CTLNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-658 262 - 312 CTLNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G I C P L S


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D P R I A Y G I C P L S


RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


RICTNCCAGKKGCKYFSNDGTFVCEGESDPRNPKACTLNCDPRIAYGICPLS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S N D G T F V C E G E S D P R N P K A C T L N C D P R I A Y G I C P L S


RICTNCCAGKKGCKYFSDDGTFVCEGESDPRNPKACTLNCDPRIAYGICPLS
CTLNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G K K G C K Y F S D D G T F V C E G E S D P R N P K A C T L N C D P R I A Y G I C P L S


KACPRNCDPRIAYGICPLSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F I C E G


KACPRNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSNDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S N D G T F V C E G


KACPLNCDPRIAYGVCPRSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P L N C D P R I A Y G V C P R S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


KACTLNCDPRIAYGICPLSEEKKNDRICTNCCAGKKGCKYFSDDGTFVCEG
CTLNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T L N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G K K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-152
 113 - 164 52
IRD-152
 55 - 106 52
IRD-153
 287 - 338 52
IRD-254
 171 - 222 52
IRD-255
 229 - 280 52
IRD-604
 88 - 138 51
IRD-606
 320 - 370 51
IRD-620
 146 - 196 51
IRD-655
 30 - 80 51
IRD-658
 204 - 254 51
IRD-658
 262 - 312 51
Amino Acid Count % by Frequency
Alanine 24 6.05
Cysteine 48 12.09
Aspartic acid 31 7.81
Glutamic acid 29 7.3
Phenylalanine 13 3.27
Glycine 32 8.06
Histidine 2 0.5
Isoleucine 18 4.53
Lysine 37 9.32
Leucine 17 4.28
Methionine 2 0.5
Asparagine 26 6.55
Proline 25 6.3
Glutamine 1 0.25
Arginine 23 5.79
Serine 24 6.05
Threonine 16 4.03
Valine 15 3.78
Tryptophan 0 0
Tyrosine 14 3.53