Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_64 [Q1WL40]  : Trypsin proteinase inhibitor

397Length

Nicotiana miersiiSpecie

PIGene Name

43649Mass

11Domain Count

Sequence :
     FASTA
MAVHRVSFLA LLLLFGMSLL VSNVEHADAK ACTFNCDPRI AYEICPRTEE KKNDRICTNC CAGRKGCKYF 70
SDDGTFVCEG ESDPKNPKGC PRNCDGRIAY GICPLSEEKK NDRICTNCCA GMKGCNYFSA DGTFVCEGEY 140
DPRNPKACPR NCDTRIAYGI CPRTEAKKND RICTNCCAGT KGCKYFSDDG TFVCEGESDP RNPKACPRNC 210
DGRIAYGICP LSEEKKNDRI CTNCCAGTKG CKYFSDDGTF VCEGESDPRN PKACPRNCDG RIAYGICPRT 280
EEKKNNQICT NCCAGTKGCN YFSANGTFIC EGESDPRNPK ACPRNCDGRI AYGICPRTEE KKNNQICTNC 350
CAGTKGCNYF SANGTFICEG ESEYVSKLDE YVHEVENDLQ KSRVAVS 397
SIGNAL PEPTIDE
SEQUENCE Position
MAVHRVSFLALLLLFGMSLLVSNVEHADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-196
229 - 280 52
IRD-197
55 - 106 52
IRD-198
113 - 164 52
IRD-199
171 - 222 52
IRD-200
287 - 338 52
IRD-533
146 - 196 51
IRD-633
88 - 138 51
IRD-634
204 - 254 51
IRD-635
262 - 312 51
IRD-635
320 - 370 51
IRD-668
30 - 80 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
6.593
6.1 -18.4 -35.8
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
Q1WL40 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428		 DQ158192
REFERENCES
PubMed ID:     16534618    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 91 - 95
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 149 - 153
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 207 - 211
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 265 - 269
RL-1 CPRNC Trypsin 323 - 327
RL-1 CPRNC Trypsin 323 - 327
RL-7 CTFNC Chymotrypsin 33 - 37
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-196 229 - 280 CPRNC 264 - 268 DPRNP 257 - 261 H-L Type (Type-I)
IRD-197 55 - 106 CPRNC 90 - 94 DPKNP 83 - 87 H-L Type (Type-I)
IRD-198 113 - 164 CPRNC 148 - 152 DPRNP 141 - 145 H-L Type (Type-I)
IRD-199 171 - 222 CPRNC 206 - 210 DPRNP 199 - 203 H-L Type (Type-I)
IRD-200 287 - 338 CPRNC 322 - 326 DPRNP 315 - 319 H-L Type (Type-I)
IRD-533 146 - 196 CPRNC 148 - 152 EAKKN 165 - 169 L-H Type (Type-II)
IRD-633 88 - 138 CPRNC 90 - 94 EEKKN 107 - 111 L-H Type (Type-II)
IRD-634 204 - 254 CPRNC 206 - 210 EEKKN 223 - 227 L-H Type (Type-II)
IRD-635 262 - 312 CPRNC 264 - 268 EEKKN 281 - 285 L-H Type (Type-II)
IRD-635 320 - 370 CPRNC 322 - 326 EEKKN 339 - 343 L-H Type (Type-II)
IRD-668 30 - 80 CTFNC 32 - 36 EEKKN 49 - 53 L-H Type (Type-II)
RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P R T


RICTNCCAGRKGCKYFSDDGTFVCEGESDPKNPKGCPRNCDGRIAYGICPLS
CPRNC
DPKNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G R K G C K Y F S D D G T F V C E G E S D P K N P K G C P R N C D G R I A Y G I C P L S


RICTNCCAGMKGCNYFSADGTFVCEGEYDPRNPKACPRNCDTRIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G M K G C N Y F S A D G T F V C E G E Y D P R N P K A C P R N C D T R I A Y G I C P R T


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


QICTNCCAGTKGCNYFSANGTFICEGESDPRNPKACPRNCDGRIAYGICPRT
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
Q I C T N C C A G T K G C N Y F S A N G T F I C E G E S D P R N P K A C P R N C D G R I A Y G I C P R T


KACPRNCDTRIAYGICPRTEAKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EAKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D T R I A Y G I C P R T E A K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KGCPRNCDGRIAYGICPLSEEKKNDRICTNCCAGMKGCNYFSADGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K G C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G M K G C N Y F S A D G T F V C E G


KACPRNCDGRIAYGICPLSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


KACPRNCDGRIAYGICPRTEEKKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R T E E K K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


KACPRNCDGRIAYGICPRTEEKKNNQICTNCCAGTKGCNYFSANGTFICEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P R T E E K K N N Q I C T N C C A G T K G C N Y F S A N G T F I C E G


KACTFNCDPRIAYEICPRTEEKKNDRICTNCCAGRKGCKYFSDDGTFVCEG
CTFNC
EEKKN
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T F N C D P R I A Y E I C P R T E E K K N D R I C T N C C A G R K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-196
 229 - 280 52
IRD-197
 55 - 106 52
IRD-198
 113 - 164 52
IRD-199
 171 - 222 52
IRD-200
 287 - 338 52
IRD-533
 146 - 196 51
IRD-633
 88 - 138 51
IRD-634
 204 - 254 51
IRD-635
 262 - 312 51
IRD-635
 320 - 370 51
IRD-668
 30 - 80 51
Amino Acid Count % by Frequency
Alanine 26 6.55
Cysteine 48 12.09
Aspartic acid 25 6.3
Glutamic acid 29 7.3
Phenylalanine 15 3.78
Glycine 35 8.82
Histidine 3 0.76
Isoleucine 20 5.04
Lysine 30 7.56
Leucine 11 2.77
Methionine 3 0.76
Asparagine 32 8.06
Proline 22 5.54
Glutamine 3 0.76
Arginine 26 6.55
Serine 19 4.79
Threonine 22 5.54
Valine 13 3.27
Tryptophan 0 0
Tyrosine 15 3.78