Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_68 [A0A1U7XTK4]  : proteinase inhibitor type-2 TR8-like

229Length

Nicotiana sylvestrisSpecie

LOC104239917Gene Name

25380Mass

4Domain Count

Sequence :
     FASTA
RICTNCCAGK KGCKYFSDDG TFICEGESEY ASKVDEYVRE VENDLQKSKV AVSEVLTNNM YGAFPLPYFS 70
DDGTFVCEGE SDPRNPKACP RNCDPRIAYG ICPLSEEKKN DRICTNCCAG TKGCKYFSDD GTFVCEGESD 140
PRNPKACPRN CDGRIAYGIC PLSKEKKNDR ICTNCCAGKK GCKYFSDDGT FICEGESEYA SKVDEYVREV 210
ENDLQKSKVA VSEVLTNNM 229
SIGNAL PEPTIDE
SEQUENCE Position
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-150
54 - 100 47
IRD-199
112 - 163 52
IRD-480
145 - 195 51
IRD-604
87 - 137 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
4.629
-5.2 -16.5 -25
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1U7XTK4 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     23773524    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 90 - 94
RL-1 CPRNC Trypsin 90 - 94
RL-1 CPRNC Trypsin 148 - 152
RL-1 CPRNC Trypsin 148 - 152
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-150 54 - 100 CPRNC 89 - 93 DPRNP 82 - 86 H-L Type (Type-I)
IRD-199 112 - 163 CPRNC 147 - 151 DPRNP 140 - 144 H-L Type (Type-I)
IRD-480 145 - 195 CPRNC 147 - 151 KEKKN 164 - 168 L-H Type (Type-II)
IRD-604 87 - 137 CPRNC 89 - 93 EEKKN 106 - 110 L-H Type (Type-II)
EVLTNNMYGAFPLPYFSDDGTFVCEGESDPRNPKACPRNCDPRIAYG
CPRNC
DPRNP
H-L Type (Type-I)


RICTNCCAGTKGCKYFSDDGTFVCEGESDPRNPKACPRNCDGRIAYGICPLS
CPRNC
DPRNP
H-L Type (Type-I)
3,40 ; 6,24 ; 7,36 ; 13,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52
R I C T N C C A G T K G C K Y F S D D G T F V C E G E S D P R N P K A C P R N C D G R I A Y G I C P L S


KACPRNCDGRIAYGICPLSKEKKNDRICTNCCAGKKGCKYFSDDGTFICEG
CPRNC
KEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D G R I A Y G I C P L S K E K K N D R I C T N C C A G K K G C K Y F S D D G T F I C E G


KACPRNCDPRIAYGICPLSEEKKNDRICTNCCAGTKGCKYFSDDGTFVCEG
CPRNC
EEKKN
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C P R N C D P R I A Y G I C P L S E E K K N D R I C T N C C A G T K G C K Y F S D D G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-150
 54 - 100 47
IRD-199
 112 - 163 52
IRD-480
 145 - 195 51
IRD-604
 87 - 137 51
Amino Acid Count % by Frequency
Alanine 12 5.24
Cysteine 22 9.61
Aspartic acid 18 7.86
Glutamic acid 21 9.17
Phenylalanine 9 3.93
Glycine 18 7.86
Histidine 0 0
Isoleucine 9 3.93
Lysine 21 9.17
Leucine 7 3.06
Methionine 2 0.87
Asparagine 15 6.55
Proline 11 4.8
Glutamine 2 0.87
Arginine 11 4.8
Serine 16 6.99
Threonine 10 4.37
Valine 14 6.11
Tryptophan 0 0
Tyrosine 11 4.8