Proteins
  2. Proteins (PIs)
  3. Proteins details
PINIR_72 [A0A1U7VHX6]  : wound-induced proteinase inhibitor 2-like

225Length

Nicotiana sylvestrisSpecie

LOC104213625Gene Name

24333Mass

4Domain Count

Sequence :
     FASTA
MAVHKVGFLS LLLLFGTFLL LSEVDYADAK ACTRNCNPRI RYGRCPKSGN KKFNVGCTNC CAGSLGCNYY 70
SANGTFVCEG ESETKVETIE TDVETIETEV ETMVESNKVC PRNCDPKIRY GRCPKSGNKK FNVGCTNCCA 140
AKKGCNYFSA NGTFVCKGES VVESMIEAVK PCRRNCDGRI SYSVCGNKKS SGRICTNCCA GKKGCNYFSA 210
DGTFVCEGES GVESI 225
SIGNAL PEPTIDE
SEQUENCE Position
MAVHKVGFLSLLLLFGTFLLLSEVDYADA
 1 ↔ 29
DOMAINS ARCHITECTURE  
Domain Position Length Architecture
IRD-39
133 - 188 56
IRD-435
170 - 218 49
IRD-522
108 - 158 51
IRD-529
30 - 80 51
ISO - ELECTRIC POINT
Iso Eelectric Point charge at pH 7.4 charge at pH 5.5 charge at pH 8.0
7.415
8.8 -3.3 -12.3
CROSS REFERENCES
Uniprot Interpro Id Pfam Id EMBL
A0A1U7VHX6 View PI in InterPro
IPR003465 		View protein in Pfam
PF02428
REFERENCES
PubMed ID:     23773524    |
Application:
REACTIVE LOOPS
Entry RCL Sequence Target Protease Position
RL-1 CPRNC Trypsin 111 - 115
RL-30 CTRNC Trypsin 33 - 37
RL-32 CRRNC Trypsin 173 - 177
RL-32 CRRNC Trypsin 173 - 177
GO TERMS
Molecular Function Cellular Component Biological Process
serine-type endopeptidase inhibitor activity (GO:0004867) None None
SPATIO TEMPORAL DISTRIBUTION
Tissue Distribution Induction Elicitor Molecules Target Insect Remarks Reference
No Records Found
BIO-CHEMICAL PROPERTIES
Ki (uM) IC50 (uM) Target Protease Cross Reactivity Remarks Reference
No Records Found
BIO-PHYSICAL PROPERTIES
Affinity Binding Energy Stability Remarks Reference
No Records Found
DOMAIN STRUCTURE FOLD
Beta Sheet Numbers Alpha Helix Numbers Coil Numbers Disulphide Bonds Numbers Remarks Reference
No Records Found
Entry Domain Position RCL RCL Position Linker Linker Position Domain Type
IRD-39 133 - 188 CRRNC 172 - 176 - H+L Type (Type-III)
IRD-435 170 - 218 CRRNC 172 - 176 - H+L Type (Type-III)
IRD-522 108 - 158 CPRNC 110 - 114 GNKKF 127 - 131 L-H Type (Type-II)
IRD-529 30 - 80 CTRNC 32 - 36 GNKKF 49 - 53 L-H Type (Type-II)
VGCTNCCAAKKGCNYFSANGTFVCKGESVVESMIEAVKPCRRNCDGRISYSVCGNK
CRRNC
H+L Type (Type-III)
3,44 ; 6,24 ; 7,40 ; 13,53
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56
V G C T N C C A A K K G C N Y F S A N G T F V C K G E S V V E S M I E A V K P C R R N C D G R I S Y S V C G N K


KPCRRNCDGRISYSVCGNKKSSGRICTNCCAGKKGCNYFSADGTFVCEG
CRRNC
H+L Type (Type-III)
3,26 ; 7,30 ; 16,36 ; 29,47
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49
K P C R R N C D G R I S Y S V C G N K K S S G R I C T N C C A G K K G C N Y F S A D G T F V C E G


KVCPRNCDPKIRYGRCPKSGNKKFNVGCTNCCAAKKGCNYFSANGTFVCKG
CPRNC
GNKKF
L-H Type (Type-II)
3,31 ; 7,32 ; 16,28 ; 38,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K V C P R N C D P K I R Y G R C P K S G N K K F N V G C T N C C A A K K G C N Y F S A N G T F V C K G


KACTRNCNPRIRYGRCPKSGNKKFNVGCTNCCAGSLGCNYYSANGTFVCEG
CTRNC
GNKKF
L-H Type (Type-II)
3,28 ; 7,32 ; 16,38 ; 31,49
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51
K A C T R N C N P R I R Y G R C P K S G N K K F N V G C T N C C A G S L G C N Y Y S A N G T F V C E G


DOMAINS ARCHITECTURE
Domain Position Length Architecture
IRD-39
 133 - 188 56
IRD-435
 170 - 218 49
IRD-522
 108 - 158 51
IRD-529
 30 - 80 51
Amino Acid Count % by Frequency
Alanine 12 5.33
Cysteine 24 10.67
Aspartic acid 6 2.67
Glutamic acid 17 7.56
Phenylalanine 10 4.44
Glycine 23 10.22
Histidine 1 0.44
Isoleucine 8 3.56
Lysine 19 8.44
Leucine 9 4
Methionine 3 1.33
Asparagine 18 8
Proline 6 2.67
Glutamine 0 0
Arginine 11 4.89
Serine 18 8
Threonine 14 6.22
Valine 18 8
Tryptophan 0 0
Tyrosine 8 3.56